Taf2 (NM_133319) Rat Tagged ORF Clone

CAT#: RR209940

Taf2 (Myc-DDK-tagged ORF) - Rat TAF2 RNA polymerase II, TATA box binding protein (TBP)-associated factor (Taf2), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Taf2
• Synonyms: TAFIIB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209940 representing NM_133319
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGCCCACCGGCTTAGAGTCCTCCAGGATGAACAGGAAGAAAGGAGACAAGGGCTTCGAAAGCCCAC
  GGCCGTACAAATTAACCCATCAGGTCGTCTGCATCAACAACATAAACTTCCAGAGAAAGTCTGTTGTGGG
  GTTTGTGGAGCTGACTATATTTCCTACGGTTGCAAACTTGAATAGAATCAAGCTGAACAGTAAACAGTGT
  AGAATATACCGAGTCAGGATCAATGACTTGGAGGCTGCTTTTATTTATAATGATCCAACCTTAGAAGTTT
  GTCACAGCGAATCAAAACAGAGAAACCTCAATTATTTTTCCAATGCGTATGCAGCTGCAGTCAGTGCTGT
  GGACCCTGATGCGGGGAACGGAGAACTCTGCATTAAGGTCCCCTCAGAGCTCTGGAAGCACGTCGATGAG
  TTAAAGGTCCTGAAGATACATATTAATTTTTCTTTGGATCAGCCAAAAGGAGGTCTTCATTTTGTGGTAC
  CCAGTGTAGAGGGGAGTATGGCAGAGAGAGGTGCTCATGTCTTCTCTTGTGGGTATCAGAATTCTACAAG
  ATTTTGGTTCCCTTGTGTTGATTCATACTCTGAGTTGTGTACCTGGAAATTAGAATTTACAGTCGATGCT
  GCGATGGTGGCTGTGTCCAATGGAGATTTGGTGGAGACAGTGTATACTCACGACATGAGAAAGAAGACCT
  TCCATTATGTGCTTGCTATTCCTACAGCAGCGTCCAATATCTCCTTGGCCATCGGGCCATTTGAAATACT
  TGTAGATCCATATATGCACGAGGTTACCCATTTTTGTTTGCCTCAACTTCTTCCATTACTTAAACATACT
  ACATCATACCTTCACGAAGTTTTTGAATTTTATGAGGAAATTTTGACTTGTCGCTATCCGTACTCCTGTT
  TTAAGACGGTCTTCATTGATGAGGCTTACGTGGAAGTGGCCGCTTATGCGTCTATGAGCATTTTCAGCAC
  AAATCTCCTGCACAGCGCCATGATTATAGATGAGACACCGCTCACCAGGCGGTGCCTCGCACAGTCTCTA
  GCACAGCAGTTCTTCGGATGTTTCATCTCCAGAATGTCTTGGTCTGATGAGTGGGTGCTGAAGGGAATTT
  CAGGTTATATTTATGGACTGTGGATGAAGAAAACGTTTGGTGTCAATGAGTACCACCATTGGATTAAAGA
  GGAACTCGATAAAATAGTGGCATATGAACTGAAAACTGGGGGAGTTTTACTGCATCCCATATTTGGTGGT
  GGAAAAGAAAAGGATAACCCCGCATCTCACCTCCACTTTTCAATAAAGCATCCACATACACTGTCTTGGG
  AATACTACACTATGTTTCAGTGTAAAGCTCACCTCGTGATGAGACTGATTGAAAACAGAATCAGCATGGA
  ATTTATGCTGCAAGTTTTCAATAAACTATTAAGTCTGGCCAGTACCGCTTCATCCCAGAAGTTCCAGTCA
  CATATGTGGAGCCAGATGCTGGTCTCCACCTACGGGTTTTTGAAGTCCATTTCCAATGTCTCTGGCAAAG
  ACATCCAGCCCTTAATAAAGCAGTGGGTAGACCAGAGTGGAGTGGTAAAGTTTTATGGAAGTTTTGCATT
  TAATAGAAAACGAAATGTTTTAGAACTAGAAATAAAACAAGATTACACATCTCCTGGCACCCAGAAGTAC
  GTGGGACCACTTAAAGTGACAGTGCAAGAGTTAGACGGCTCCTTCAACCATACCTTGCAAATTGAAGAAA
  ACAGCCTTAAACATGACATACCCTGCCACTCCAAAAGCAGAAGGAATAAGAAGAAGAAAATCCCACTGAT
  GAATGGGGAAGAAGTCGATATGGATCTTTCTGCAATGGATGCGGACTCCCCTTTGCTGTGGATAAGGATA
  GACCCAGATATGTCTGTCCTGAGGAAGGTGGAGTTTGAGCAGGCTGACTTTATGTGGCAGTATGAGCTCC
  GCTATGAGAGAGATGTTGTTGCTCAGCAGGAATCCATCTTGGCCTTGGAAAAGTTCCCCACGCCCGCATC
  GCGCCTCGCGCTCACTGATATACTAGAGCAAGAGCAGTGTTTCTACCGAGTGAGGATGTCAGCATGCTTC
  TGCCTTGCAAAGATTGCAAACTCAATGGTGAGCACATGGACGGGACCACCAGCCATGAAGTCCCTCTTTA
  CTAGAATGTTTTGTTGTAAAACTTGTCCCAACATCGTGAAAACAAACAACTTCATGAGCTTTCAGAGTTA
  TTTTCTACAGAAGACTATGCCAGTTGCAATGGCTTTGTTAAGAGACGTACATAATCTTTGTCCCAAAGAA
  GTTTTAACATTTATTTTAGACTTAATCAAGTACAATGACAACAGAAAAAATAAGTTTTCAGATAACTATT
  ACCGTGCAGAAATGATTGATGCCTTGGCTAACTCTGTCACACCTGCTGTCAGCGTGAATAATGAAGTCAG
  AACTTTGGATAACTTAAATCCTGATGTGCGACTAATACTTGAAGAAATTACCCGGTTTTTGAATATGGAA
  AAACTTCTCCCAAGTTACAGACATACTATCACTGTCAGTTGTTTGAGAGCCATAAGAGTGCTTCAGAAGA
  ATGGACATGTGCCAAGTGATGCATCTCTTTTCAAATCCTATGCTGAGTATGGCCACTTCGTGGACATCAG
  GATAGCGGCTCTGGAAGCCGTCGTCGATTACACCAAAGTGGACAGGAGTTATGAAGAACTTCAATGGCTA
  CTTAATATGATTCAGACTGACCCTGTCCCCTATGTGAGGCATAAGATTCTAAACATGCTGACTAAAAATC
  CGCCATTTACAAAGAACATGGAGTCTCCTTTATGCAACGAGGCCTTGGTAGATCAGCTCTGGAAGCTGAT
  GAATTCTGGTACTGCACATGACTGGAGGTTACGGTGTGGAGCTGTGGACTTGTACTTCACGCTCTTTGGC
  CTCAGTAGACCTTCTTGTTTACCCTTGCCAGAGCTCGGGCTTGTTCTTAACCTAAAAGAGAAGAAAGCTG
  TCTTGAATCCAACCATAATTCCAGAAGCGGGAGTAGGCAATCAGGAAACTGCAAGTAACCCAGGCTGTCA
  CACTCAGCTAGCAGGATTTCAGAACCCTTTCTCAAGTTCTCAGGATGAGGAGGAGGTCGATATGGACACC
  GTTCATGATAGTCAGGCCTTCATTTCCCATCATCTGAACATGCTTGAAAGGCCGTCCACTCCAGGGCTCT
  CTAAGTACCGACCATCCAGCTCCAGGTCTTCCTTAATGCCCCAGCATGCATTAGGCTGTGACATCACACC
  AACCACAAAACCCCAGTGGAGTATGGAACTGTCCCGAAAGGGAGCAGGTAAAGAGCAGCCTTTGGAGATG
  GGTGTGCATCCCGTGGCGGCCCCGCTCTCCGTGTTTGCTAAGGAGGCTCTGTCCTCTGCCCGGCACAGCG
  AGCACCACCACCACCATCACCATGAGCACAAGAAGAAGAAGAAGAAGCACAAACACAAGCACAAGCACAA
  GCACAAGCATGACGGCAAGGACAAGGACAAGGACAAGGAGCCCTTCACCTTCTCCAGCCCTGCCAGTGGC
  AGGTCTGTGCGCTCCCCTTCACTCTCAGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209940 representing NM_133319
  Red=Cloning site Green=Tags(s)
  
  MPPTGLESSRMNRKKGDKGFESPRPYKLTHQVVCINNINFQRKSVVGFVELTIFPTVANLNRIKLNSKQC
  RIYRVRINDLEAAFIYNDPTLEVCHSESKQRNLNYFSNAYAAAVSAVDPDAGNGELCIKVPSELWKHVDE
  LKVLKIHINFSLDQPKGGLHFVVPSVEGSMAERGAHVFSCGYQNSTRFWFPCVDSYSELCTWKLEFTVDA
  AMVAVSNGDLVETVYTHDMRKKTFHYVLAIPTAASNISLAIGPFEILVDPYMHEVTHFCLPQLLPLLKHT
  TSYLHEVFEFYEEILTCRYPYSCFKTVFIDEAYVEVAAYASMSIFSTNLLHSAMIIDETPLTRRCLAQSL
  AQQFFGCFISRMSWSDEWVLKGISGYIYGLWMKKTFGVNEYHHWIKEELDKIVAYELKTGGVLLHPIFGG
  GKEKDNPASHLHFSIKHPHTLSWEYYTMFQCKAHLVMRLIENRISMEFMLQVFNKLLSLASTASSQKFQS
  HMWSQMLVSTYGFLKSISNVSGKDIQPLIKQWVDQSGVVKFYGSFAFNRKRNVLELEIKQDYTSPGTQKY
  VGPLKVTVQELDGSFNHTLQIEENSLKHDIPCHSKSRRNKKKKIPLMNGEEVDMDLSAMDADSPLLWIRI
  DPDMSVLRKVEFEQADFMWQYELRYERDVVAQQESILALEKFPTPASRLALTDILEQEQCFYRVRMSACF
  CLAKIANSMVSTWTGPPAMKSLFTRMFCCKTCPNIVKTNNFMSFQSYFLQKTMPVAMALLRDVHNLCPKE
  VLTFILDLIKYNDNRKNKFSDNYYRAEMIDALANSVTPAVSVNNEVRTLDNLNPDVRLILEEITRFLNME
  KLLPSYRHTITVSCLRAIRVLQKNGHVPSDASLFKSYAEYGHFVDIRIAALEAVVDYTKVDRSYEELQWL
  LNMIQTDPVPYVRHKILNMLTKNPPFTKNMESPLCNEALVDQLWKLMNSGTAHDWRLRCGAVDLYFTLFG
  LSRPSCLPLPELGLVLNLKEKKAVLNPTIIPEAGVGNQETASNPGCHTQLAGFQNPFSSSQDEEEVDMDT
  VHDSQAFISHHLNMLERPSTPGLSKYRPSSSRSSLMPQHALGCDITPTTKPQWSMELSRKGAGKEQPLEM
  GVHPVAAPLSVFAKEALSSARHSEHHHHHHHEHKKKKKKHKHKHKHKHKHDGKDKDKDKEPFTFSSPASG
  RSVRSPSLSD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133319
• ORF Size: 3600 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_133319.1, NP_579853.1
• RefSeq Size: 5042
• RefSeq ORF: 3603
• Locus ID: 170844
• MW: 137.1 kDa
• Gene Summary: mRNA expression increases in response to paraquat intake; may play a role in response to paraquat induced reactive oxygen species production and lung injury [RGD, Feb 2006]