Gpd2 (NM_012736) Rat Tagged ORF Clone

CAT#: RR210431

Gpd2 (Myc-DDK-tagged ORF) - Rat glycerol-3-phosphate dehydrogenase 2, mitochondrial (Gpd2), nuclear gene encoding mitochondrial protein, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gpd2
• Synonyms: mtGPDH
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210431 representing NM_012736
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTTTCAAAAGGTAGTGAAAGGGACTATTCTTATGGGTGGAGGAGCTCTGGCCACTGTTTTGGGAC
  TCTCTCAGTTTGCTCACTACAGAAGGAAGCAAGTAAGCCTGGCGTATGTGGAAGCAGCAACATGCTTCTC
  AGAGCCCGTGAACAGGGAGCCTCCTTCCAGAGAAGCTCAGCTCATGACTCTGCAGAACACATCGGAATTT
  GACATCCTCGTCATTGGAGGAGGAGCCACAGGATGTGGCTGTGCGCTAGACGCCGTCACCAGAGGACTGA
  AAACAGCCCTTGTAGAGAGAAATGACTTCGCATCAGGGACTAGCAGCAGAAGCACTAAATTGATCCATGG
  TGGCGTACGATACCTCCAGAAGGCTATCACGAACTTGGATGTTGAACAGTATAGGATGGTGAAAGAGGCC
  CTTCATGAACGTGCCAACTTACTAGAAATCGCTCCCCATCTATCAGCTCCGCTGCCTATAATGCTGCCAC
  TTTATAAGTGGTGGCAGTTACCTTATTACTGGGTGGGAATCAAGATGTATGATCTGGTTGCAGGAAGTCA
  TTGCCTGAAGAGCAGTTATGTCCTCAGTAAATCCAGAGCCCTTGAGCATTTCCCCATGCTCCAGAAGGAC
  AAGCTGGTCGGAGCCATTGTCTACTATGATGGACAGCACAATGACGCCCGGATGAACCTCGCCATCGCCC
  TCACCGCTGCCAGGTACGGGGCTGCCACGGCCAATTACATGGAGGTGGTGAGCTTGCTCAAGAAGACAGA
  CCCCGAAACTGGGAAAGAGCGCGTGAGTGGTGCCCGGTGCAAGGACGTGCTCACAGGGCATGAGTTTAAC
  GTGAGAGCTAAATGCGTTATCAATGCCACCGGCCCTTTCACAGACTCCGTGCGCAAAATGGATGATAACG
  ATGTTGTTCCCATCTGCCAGCCCAGTGCAGGGGTTCACATTGTGATGCCCGGGTACTACAGCCCCGAGAA
  CATGGGACTTCTTGATCCTGCAACCAGTGATGGCAGAGTTATTTTCTTCTTGCCTTGGGAGAAGATGACA
  ATTGCGGGTACCACTGATTCGCCAACGGACGTCACGCACCATCCTATTCCTTCGGAAGACGACATTAACT
  TCATCTTAAATGAAGTGCGGAACTACCTGAGTTGCGACGTTGAAGTGAGAAGAGGGGATGTCCTGGCAGC
  GTGGAGTGGCATTCGCCCGCTTGTTACCGATCCCAAGTCTGCAAACACTCAGTCCATCTCTCGAAATCAT
  GTTGTGGAAGTCAGCGACAGTGGCCTCATCACAATAGCAGGTGGGAAGTGGACCACATATCGCTCCATGG
  CAGAAGATACTGTGAACAAAGCTGTCAAGCTTCACAACTTGAATGCAGGACCCAGTAGGACCGTTGGGCT
  CTTCCTTCAAGGAGGCAAAGATTGGAGCCCCACACTCTACATCAGGCTTGTCCAGGACTATGGGCTTGAG
  AGTGAGGTTGCACAACACCTGGCCAAAACCTATGGTGACAAGGCTTTCGACGTGGCCAAAATGGCAAGTG
  TGACTGGAAAACGGTGGCCTGTTGTTGGAGTGCGTCTTGTGTCAGAATTTCCGTACATCGAAGCAGAGGT
  GAAATATGGGATTAAGGAGTACGCATGCACCGCTGTTGACATGATTTCACGTCGCACCCGCCTGGCCTTT
  CTTAATGTTCAGGCTGCAGAGGAAGCCCTGCCCAAGATTGTTGAGTTAATGGGAAGAGAATTGAACTGGA
  GTGAATTGAGGAAACAGGAAGAACTTGAAACAGCCACGAGGTTTCTGTACTATGAAATGGGCTATAAGTC
  TCGAACAGAACAACTCACAGATAGTACTGAAATCAGCCTGCTGCCTCCAGACATCGATCGGTACAAGAAG
  AGATTTCACATGTTTGATGAAGACGAAAAAGGCTTCATTACCATTGTTGATGTTCAACGTGTGCTAGAGA
  GTATCAATGTACAAATGGACGAAGACACACTACATGAAATTCTCTGTGAAGTAGACTTGAACAAGAATGG
  TCAGGTTGAGCTCCATGAGTTTCTGCAGCTGATGAGCGCAGTTCATACAGGAAGGGTGTCTGGAAGCCGG
  CTTGCCATCCTCATGAAGACTGCCGAGGAGAACTTGGACCGTAGAGTTCCAATCCCCGTGGATCGTAGTT
  GTGGAGGATTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210431 representing NM_012736
  Red=Cloning site Green=Tags(s)
  
  MAFQKVVKGTILMGGGALATVLGLSQFAHYRRKQVSLAYVEAATCFSEPVNREPPSREAQLMTLQNTSEF
  DILVIGGGATGCGCALDAVTRGLKTALVERNDFASGTSSRSTKLIHGGVRYLQKAITNLDVEQYRMVKEA
  LHERANLLEIAPHLSAPLPIMLPLYKWWQLPYYWVGIKMYDLVAGSHCLKSSYVLSKSRALEHFPMLQKD
  KLVGAIVYYDGQHNDARMNLAIALTAARYGAATANYMEVVSLLKKTDPETGKERVSGARCKDVLTGHEFN
  VRAKCVINATGPFTDSVRKMDDNDVVPICQPSAGVHIVMPGYYSPENMGLLDPATSDGRVIFFLPWEKMT
  IAGTTDSPTDVTHHPIPSEDDINFILNEVRNYLSCDVEVRRGDVLAAWSGIRPLVTDPKSANTQSISRNH
  VVEVSDSGLITIAGGKWTTYRSMAEDTVNKAVKLHNLNAGPSRTVGLFLQGGKDWSPTLYIRLVQDYGLE
  SEVAQHLAKTYGDKAFDVAKMASVTGKRWPVVGVRLVSEFPYIEAEVKYGIKEYACTAVDMISRRTRLAF
  LNVQAAEEALPKIVELMGRELNWSELRKQEELETATRFLYYEMGYKSRTEQLTDSTEISLLPPDIDRYKK
  RFHMFDEDEKGFITIVDVQRVLESINVQMDEDTLHEILCEVDLNKNGQVELHEFLQLMSAVHTGRVSGSR
  LAILMKTAEENLDRRVPIPVDRSCGGL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012736
• ORF Size: 2181 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_012736.1, NP_036868.1
• RefSeq Size: 2400
• RefSeq ORF: 2184
• Locus ID: 25062
• MW: 81 kDa
• Gene Summary: FAD-linked glycerol-3-phosphate dehydrogenase; expression in liver is induced by thyroid hormone [RGD, Feb 2006]