Ucp3 (NM_013167) Rat Tagged ORF Clone

CAT#: RR210529

Ucp3 (Myc-DDK-tagged ORF) - Rat uncoupling protein 3 (mitochondrial, proton carrier) (Ucp3), nuclear gene encoding mitochondrial protein, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ucp3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210529 representing NM_013167
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTGGACTTCAGCCATCAGAAGTGCCTCCCACAACAGTTGTAAAGTTCCTGGGGGCCGGCACTGCAG
  CCTGTTTTGCTGATCTCCTCACCTTCCCCCTGGACACCGCCAAGGTCCGCCTGCAGATCCAAGGGGAGAA
  CCCAGGAGTGCAGAGCGTGCAGTACCGCGGCGTGCTCGGTACCATCCTGACTATGGTGCGCACAGAGGGT
  CCCCGCAGCCCCTACAGCGGGCTGGTCGCTGGCCTGCATCGCCAGATGAGTTTTGCCTCCATTCGAATTG
  GCCTCTACGACTCTGTCAAGCAGTTCTACACCCCCAAAGGAACGGACCACTCCAGCGTCGCCATCAGGAT
  TCTGGCAGGCTGCACCACGGGAGCCATGGCAGTGACCTGTGCTCAACCCACGGATGTGGTGAAGGTCCGA
  TTTCAAGCCATGATACGCCTGGGAACTGGAGGCGAGAGGAAATACAGAGGGACTATGGATGCCTACAGAA
  CCATCGCCAGGGAAGAAGGAGTCAGGGGACTGTGGAAAGGGACTTGGCCCAACATCACAAGAAACGCCAT
  TGTCAATTGTGCTGAGATGGTGACCTATGACATCATCAAGGAGAAGCTGCTGGACTCTCACCTGTTCACT
  GACAACTTCCCCTGTCACTTTGTCTCTGCCTTTGGAGCTGGTTTCTGTGCCACGGTGGTGGCCTCCCCAG
  TGGATGTGGTAAAGACCCGATACATGAACGCTCCCCCAGGCAGGTACCGAAGCCCCCTACACTGTATGCT
  GAGGATGGTGGCCCAGGAGGGCCCCACAGCCTTCTACAAAGGATTCATGCCCTCCTTTCTGCGTCTGGGA
  TCCTGGAACGTGATGATGTTTGTAACCTACGAGCAGCTGAAACGGGCCTTGATGAAAGTCCAGGTACTGC
  GGGAATCTCCATTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210529 representing NM_013167
  Red=Cloning site Green=Tags(s)
  
  MVGLQPSEVPPTTVVKFLGAGTAACFADLLTFPLDTAKVRLQIQGENPGVQSVQYRGVLGTILTMVRTEG
  PRSPYSGLVAGLHRQMSFASIRIGLYDSVKQFYTPKGTDHSSVAIRILAGCTTGAMAVTCAQPTDVVKVR
  FQAMIRLGTGGERKYRGTMDAYRTIAREEGVRGLWKGTWPNITRNAIVNCAEMVTYDIIKEKLLDSHLFT
  DNFPCHFVSAFGAGFCATVVASPVDVVKTRYMNAPPGRYRSPLHCMLRMVAQEGPTAFYKGFMPSFLRLG
  SWNVMMFVTYEQLKRALMKVQVLRESPF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013167
• ORF Size: 924 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_013167.1, NM_013167.2, NP_037299.1
• RefSeq Size: 2395
• RefSeq ORF: 927
• Locus ID: 25708
• MW: 34 kDa
• Gene Summary: catalyzes a proton leak across the inner mitochondrial membrane to uncouple oxidative phosphorylation; may reduce the concentration of reactive ozygen species in mitochondria [RGD, Feb 2006]