Cpsf3 (NM_001030030) Rat Tagged ORF Clone

CAT#: RR210550

Cpsf3 (Myc-DDK-tagged ORF) - Rat cleavage and polyadenylation specificity factor 3 (Cpsf3), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cpsf3
• Synonyms: MGC124885
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210550 representing NM_001030030
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGCAATTCCTGCAGAGGAGAGCGACCAGCTGCTGATCCGACCCCTTGGTGCTGGCCAAGAAGTGG
  GAAGATCATGTATTATTCTGGAGTTCAAAGGAAGGAAAATCATGCTAGACTGTGGGATCCACCCTGGCCT
  GGAAGGAATGGATGCTCTTCCTTACATTGACCTCATCGACCCAGCTGAGATCGACCTTCTGTTGATCAGT
  CACTTCCATTTGGACCACTGTGGAGCCCTGCCCTGGTTCCTTCAGAAGACAAGTTTCAAAGGACGGACGT
  TTATGACCCACGCCACAAAAGCTATCTATAGATGGCTCCTCTCAGACTATGTCAAAGTCAGCAACATATC
  AGCAGATGACATGCTGTACACTGAGACAGACTTGGAAGAAAGCATGGACAAAATCGAAACCATCAATTTC
  CATGAAGTTAAGGAAGTTGCAGGAATCAAGTTCTGGTGTTACCATGCCGGCCACGTCCTGGGAGCAGCCA
  TGTTTATGATAGAGATTGCTGGTATGAAGCTTTTGTATACAGGTGATTTCTCAAGACAAGAGGACAGACA
  TCTAATGGCCGCTGAAATTCCTAATATTAAGCCAGACATCCTGATCATTGAATCTACGTATGGGACCCAT
  ATCCATGAGAAGCGTGAAGAGCGAGAAGCAAGATTCTGCAACACTGTGCACGACATTGTCAACAGAGGGG
  GCAGAGGGCTCATTCCTGTCTTTGCTCTCGGAAGAGCTCAGGAGCTGCTCCTGATTCTAGATGAGTACTG
  GCAGAACCACCCAGAGCTGCACGACATCCCCATTTACTACGCATCGTCTCTGGCCAAGAAGTGCATGGCC
  GTGTACCAGACCTACGTGAACGCCATGAACGACAAGATCCGGAAGCAGATCAACATCAACAACCCCTTCG
  TTTTCAAGCACATCAGCAACCTCAAGAGCATGGATCACTTTGACGACATTGGCCCCAGTGTTGTAATGGC
  CTCACCAGGCATGATTCAAAATGGCTTATCCAGAGAGCTGTTTGAAAGCTGGTGTACAGATAAGAGGAAT
  GGCGTCATCATCGCAGGGTACTGTGTAGAAGGGACACTTGCCAAGCACATCATGTCTGAACCTGAAGAAA
  TCACTACCATGTCTGGACAGAAGTTGCCACTGAAAATGTCGGTTGATTACATTTCTTTCTCTGCTCACAC
  AGATTACCAGCAAACCAGTGAATTTATTCGTGCTCTGAAGCCGCCTCACGTGATTTTAGTCCATGGGGAA
  CAGAATGAAATGGCCAGACTGAAAGCAGCACTGATCCGAGAATATGAAGATAACGATGAAGTTCACATCG
  AGGTTCACAATCCTCGGAACACAGAGGCCGTGACCCTGAACTTCAGAGGGGAGAAGCTCGCCAAGGTCAT
  GGGCTTTTTAGCAGACAAGAAGCCAGAACAGGGCCAGCGGGTCTCTGGAATTCTTGTTAAAAGAAACTTT
  AATTACCACATACTCTCTCCCTGTGACCTCTCGAATTACACTGACCTGGCCATGAGCACTGTGAAACAGA
  CCCAAGCCATCCCATATACTGGGCCCTTTTACCTGCTCTATTACCAGCTACAGAAATTGACGGGTGATGT
  AGAAGAATTAGAAATTCAAGAAAAGCCTGCTTTGAAGGTATTCAAAAGCATCACCGTGGTGCAGGAGCCG
  GGCATGGTGGTCCTGGAGTGGCTGGCGAACCCCTCTAACGACATGTATGCAGACACAGTGACCACCGTGA
  TATTGGAAGTTCAGTCGAATCCAAAAATAAGGAAAGGTGCAGTGCAGAAGGTCTCTAAGAAGCTAGAGAT
  GCACGTTTACAGCAAGAGGCTTGAGGTCATGCTCCAGGACATATTTGGAGAAGACTGTGTCAGTGTGAAA
  GATGACTCTGTCCTCAGTGTCACGGTGGATGGAAAAACAGCAAATATTAATTTGGAGACTCGGGCTGTAG
  AATGTGAAGAAGGAAGTGAGGATGATGAGTCCCTCCGAGAAATGGTGGAACTGGCCGCCCAGAGACTGTA
  CGAGGCTCTGACGCCGCCAGTGCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210550 representing NM_001030030
  Red=Cloning site Green=Tags(s)
  
  MSAIPAEESDQLLIRPLGAGQEVGRSCIILEFKGRKIMLDCGIHPGLEGMDALPYIDLIDPAEIDLLLIS
  HFHLDHCGALPWFLQKTSFKGRTFMTHATKAIYRWLLSDYVKVSNISADDMLYTETDLEESMDKIETINF
  HEVKEVAGIKFWCYHAGHVLGAAMFMIEIAGMKLLYTGDFSRQEDRHLMAAEIPNIKPDILIIESTYGTH
  IHEKREEREARFCNTVHDIVNRGGRGLIPVFALGRAQELLLILDEYWQNHPELHDIPIYYASSLAKKCMA
  VYQTYVNAMNDKIRKQININNPFVFKHISNLKSMDHFDDIGPSVVMASPGMIQNGLSRELFESWCTDKRN
  GVIIAGYCVEGTLAKHIMSEPEEITTMSGQKLPLKMSVDYISFSAHTDYQQTSEFIRALKPPHVILVHGE
  QNEMARLKAALIREYEDNDEVHIEVHNPRNTEAVTLNFRGEKLAKVMGFLADKKPEQGQRVSGILVKRNF
  NYHILSPCDLSNYTDLAMSTVKQTQAIPYTGPFYLLYYQLQKLTGDVEELEIQEKPALKVFKSITVVQEP
  GMVVLEWLANPSNDMYADTVTTVILEVQSNPKIRKGAVQKVSKKLEMHVYSKRLEVMLQDIFGEDCVSVK
  DDSVLSVTVDGKTANINLETRAVECEEGSEDDESLREMVELAAQRLYEALTPPVH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001030030
• ORF Size: 2055 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001030030.1, NP_001025201.1
• RefSeq Size: 2248 bp
• RefSeq ORF: 2058 bp
• Locus ID: 298916
• Cytogenetics: 6q16
• MW: 77.6 kDa