Hck (NM_013185) Rat Tagged ORF Clone

CAT#: RR210580

Hck (Myc-DDK-tagged ORF) - Rat hemopoietic cell kinase (Hck), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hck
• Synonyms: Hctk; p56Hck; p59Hck
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210580 representing NM_013185
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  CTGGGGGGTCGTTCGAGCTGCGAGGATCCGGGCTGCCCGCGAGGCGAAGGGCGGGTGCCCAGGATGGGAT
  GTGTGAAGTCCAGGTTCCTCCGAGAAGGAAGCAAGGCCTCAAAAATAGAGCCAAATGCCAACCAGAAAGG
  CCCTGTGTATGTGCCGGATCCCACGTCCCCTAAGAAGCTGGGACCGAACAGCATCAACAGCCTGCCCCCG
  GGGGTCGTGGAGGGCTCTGAGGACACCATTGTGGTCGCACTGTACGACTATGAGGCCATTCACCGTGAAG
  ACCTCAGCTTCCAGAAGGGAGACCAGATGGTGGTTCTGGAGGAGTCTGGGGAGTGGTGGAAGGCCCGTTC
  CCTGGCTACCAAGAAAGAAGGCTATATCCCAAGCAATTATGTAGCTCGAGTTAACTCTTTGGAGACTGAG
  GAGTGGTTCTTCAAGGGTATCAGCCGGAAGGATGCAGAGCGCCACCTGCTGGCTCCCGGGAACATGCTGG
  GCTCCTTCATGATCCGGGACAGTGAGACCACCAAAGGGAGCTACTCACTTTCTGTTCGAGACTTTGACCC
  CCAGCACGGAGACACGGTGAAGCATTATAAAATCCGGACACTGGACAGTGGAGGCTTCTACATCTCTCCG
  AGGAGCACCTTCAGCAGCCTGCAGGAACTTGTCGTCCACTACAAGAAGGGGAAGGATGGGCTCTGCCAGA
  AGCTGTCAGTGCCCTGTGTGTCTCCCAAACCCCAGAAGCCATGGGAGAAAGATGCCTGGGAGATTCCTCG
  AGAATCCCTCCAGATGGAGAAGAAACTGGGAGCCGGGCAGTTTGGAGAAGTGTGGATGGCCACCTACAAC
  AAGCACACCAAAGTGGCGGTGAAGACAATGAAGCCAGGGAGCATGTCTGTGGAGGCCTTCCTGGCAGAGG
  CCAACCTGATGAAGACGTTACAGCATGATAAACTGGTGAAGCTGCACGCCGTGGTCTCTCAGGAGCCCAT
  CTTTATTGTCACCGAGTTCATGGCCAAAGGAAGCCTGCTGGACTTTCTCAAGAGTGAAGAAGGCAGCAAG
  CAGCCACTGCCAAAACTCATTGACTTCTCAGCCCAGATTTCAGAGGGCATGGCTTTCATTGAGCAGAGGA
  ACTACATCCACCGAGACCTCCGGGCTGCCAACATCTTGGTTTCTGCATCACTGGTGTGTAAGATCGCTGA
  CTTTGGACTGGCACGGATCATCGAGGACAATGAGTACACAGCTCGGGAAGGAGCCAAGTTCCCCATCAAG
  TGGACAGCTCCTGAAGCCATCAACTTTGGCTCCTTCACCATCAAGTCAGATGTCTGGTCCTTTGGTATCC
  TGCTGATGGAAATCGTCACCTACGGCCGGATCCCTTACCCAGGTATGTCAAACCCAGAGGTGATTCGAGC
  ACTAGAGCATGGGTACCGTATGCCTCGACCAGATAACTGCCCAGAGGAGCTCTACAGTATCATGATCCGC
  TGCTGGAAGAACCGTCCAGAGGAACGGCCCACTTTCGAATACATCCAGAGCGTGCTGGATGACTTCTACA
  CGGCCACTGAGAGCCAGTATCAGCAGCAACCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210580 representing NM_013185
  Red=Cloning site Green=Tags(s)
  
  LGGRSSCEDPGCPRGEGRVPRMGCVKSRFLREGSKASKIEPNANQKGPVYVPDPTSPKKLGPNSINSLPP
  GVVEGSEDTIVVALYDYEAIHREDLSFQKGDQMVVLEESGEWWKARSLATKKEGYIPSNYVARVNSLETE
  EWFFKGISRKDAERHLLAPGNMLGSFMIRDSETTKGSYSLSVRDFDPQHGDTVKHYKIRTLDSGGFYISP
  RSTFSSLQELVVHYKKGKDGLCQKLSVPCVSPKPQKPWEKDAWEIPRESLQMEKKLGAGQFGEVWMATYN
  KHTKVAVKTMKPGSMSVEAFLAEANLMKTLQHDKLVKLHAVVSQEPIFIVTEFMAKGSLLDFLKSEEGSK
  QPLPKLIDFSAQISEGMAFIEQRNYIHRDLRAANILVSASLVCKIADFGLARIIEDNEYTAREGAKFPIK
  WTAPEAINFGSFTIKSDVWSFGILLMEIVTYGRIPYPGMSNPEVIRALEHGYRMPRPDNCPEELYSIMIR
  CWKNRPEERPTFEYIQSVLDDFYTATESQYQQQP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013185
• ORF Size: 1572 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_013185.2, NM_013185.3, NP_037317.2
• RefSeq Size: 2081
• RefSeq ORF: 1575
• Locus ID: 25734
• MW: 59.1 kDa
• Gene Summary: The protein encoded by this gene is a protein-tyrosine kinase that is predominantly expressed in hemopoietic cell types.The encoded protein may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Alternate translation initiation site usage, including a non-AUG (CUG) codon, results in the production of two different isoforms in human and mouse. Based on strong sequence conservation amongst these species, this may also be true for rat. [provided by RefSeq, Jul 2008]