Hmgcs1 (NM_017268) Rat Tagged ORF Clone

CAT#: RR210877

Hmgcs1 (Myc-DDK-tagged ORF) - Rat 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1 (soluble) (Hmgcs1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hmgcs1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210877 representing NM_017268
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTGGGTCACTTCCTTTGAATGCAGAGGCTTGCTGGCCAAAAGATGTGGGAATCGTTGCCCTTGAAA
  TCTACTTTCCTTCTCAGTATGTTGATCAAGCTGAGTTGGAAAAATACGATGGTGTAGATGCTGGAAAGTA
  TACCATTGGCCTGGGCCAGGCCAGGATGGGCTTCTGCACGGATCGCGAAGACATCAACTCTCTCTGCCTG
  ACTGTGGTTCAGAAACTGATGGAGAGAAATAGCCTTTCCTATGACTGCATTGGGCGGCTGGAAGTCGGGA
  CAGAGACAATCATCGACAAATCAAAATCCGTGAAGTCTAATTTGATGCAGCTGTTTGAGGAGTCTGGGAA
  TACAGATATAGAAGGAATAGATACAACTAATGCATGCTATGGGGGCACAGCCGCAGTCTTCAATGCTGTG
  AACTGGATCGAATCCAGCTCTTGGGATGGACGATACGCTTTGGTAGTTGCAGGAGACATCGCTATATATG
  CCTCAGGAAACGCCAGGCCTACAGGTGGAGTTGGAGCTGTGGCTCTGCTAATTGGGCCAAATGCTCCTGT
  AATTTTTGACCGAGGGCTTCGTGGGACACACATGCAGCATGCCTACGACTTTTACAAGCCTGACATGCTC
  TCTGAATACCCTGTGGTAGATGGAAAACTCTCCATACAGTGCTACCTCAGCGCATTGGACCGCTGCTATT
  CTGTCTACCGCAAAAAGATCCGGGCCCAGTGGCAGAAAGAGGGAAAGGATAAAGATTTTACCCTGAATGA
  TTTTGGCTTCATGATCTTTCACTCGCCATACTGTAAACTGGTGCAGAAATCTCTAGCTAGGATGTTCCTG
  AATGACTTTCTTAACGATCAAAACAGAGACAAAAACAGTATTTACAGTGGGCTGGAAGCCTTTGGGGATG
  TGAAATTAGAAGATACTTACTTCGACAGAGATGTGGAAAAGGCATTTATGAAGGCTAGTGCTGAGCTATT
  CAACCAGAAAACAAAGGCATCTTTGCTTGTATCGAATCAAAATGGAAACATGTACACATCCTCTGTATAC
  GGTTCCCTTGCTTCTGTTCTGGCACAGTACTCACCTCAACAGTTGGCCGGGAAGAGGATTGGAGTGTTCT
  CTTACGGTTCTGGCTTGGCTGCCACACTCTACTCCCTTAAAGTCACACAAGATGCCACACCAGGATCTGC
  TCTTGACAAAATAACAGCAAGTTTATGTGACCTTAAGTCAAGGCTTGACTCAAGAACGTGTGTGGCACCA
  GACGTCTTTGCTGAAAACATGAAGCTCAGAGAGGACACACATCACTTAGCCAACTATATTCCCCAGTGTT
  CAATAGATTCACTCTTCGAAGGAACATGGTATCTAGTCAGAGTGGATGAAAAGCACAGAAGAACTTACGC
  CCGGCGTCCCTCCACAAATGACCACAGTTTGGATGAAGGAGTGGGACTTGTGCATTCAAACACAGCTACA
  GAGCATATTCCAAGCCCGGCTAAGAAAGTGCCAAGACTTCCTGCGACCTCGGGCGAACCTGAGTCGGCTG
  TCATCAGTAACGGGGAGCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210877 representing NM_017268
  Red=Cloning site Green=Tags(s)
  
  MPGSLPLNAEACWPKDVGIVALEIYFPSQYVDQAELEKYDGVDAGKYTIGLGQARMGFCTDREDINSLCL
  TVVQKLMERNSLSYDCIGRLEVGTETIIDKSKSVKSNLMQLFEESGNTDIEGIDTTNACYGGTAAVFNAV
  NWIESSSWDGRYALVVAGDIAIYASGNARPTGGVGAVALLIGPNAPVIFDRGLRGTHMQHAYDFYKPDML
  SEYPVVDGKLSIQCYLSALDRCYSVYRKKIRAQWQKEGKDKDFTLNDFGFMIFHSPYCKLVQKSLARMFL
  NDFLNDQNRDKNSIYSGLEAFGDVKLEDTYFDRDVEKAFMKASAELFNQKTKASLLVSNQNGNMYTSSVY
  GSLASVLAQYSPQQLAGKRIGVFSYGSGLAATLYSLKVTQDATPGSALDKITASLCDLKSRLDSRTCVAP
  DVFAENMKLREDTHHLANYIPQCSIDSLFEGTWYLVRVDEKHRRTYARRPSTNDHSLDEGVGLVHSNTAT
  EHIPSPAKKVPRLPATSGEPESAVISNGEH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017268
• ORF Size: 1560 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_017268.1, NP_058964.1
• RefSeq Size: 3275
• RefSeq ORF: 1563
• Locus ID: 29637
• MW: 57.4 kDa
• Gene Summary: catalyzes the conversion of (S)-3-hydroxy-3-methylglutaryl-CoA and CoA to acetyl-CoA, acetoacetyl-CoA, and H2O [RGD, Feb 2006]