Pspc1 (NM_001025672) Rat Tagged ORF Clone

CAT#: RR210895

Pspc1 (Myc-DDK-tagged ORF) - Rat paraspeckle component 1 (Pspc1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pspc1
• Synonyms: MGC116378
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210895 representing NM_001025672
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGTTAAGAGGAAACCTGAAGCAAGTACGTATTGAGAAAAATCCGGCTCGCCTTCGCGCCCTGGAGT
  CGGCGGCCGGAGAGAGCGAGCCGGTGGCCGCGGCGGCCATGGCGCTGACGCTGGCCGGGGAGCAGCCGCC
  GCCACCCGCTCCTTCGGAGGAGCACCCGGACGAGGAGATGGGCTTCACCATTGACATCAAGAGTTTCCTC
  AAGCCCGGCGAGAAGACGTACACGCAGCGCTGCCGCCTCTTCGTGGGTAACCTGCCCACCGACATCACGG
  AGGAGGACTTCAAGCGGCTCTTTGAGCGCTACGGCGAGCCCAGCGAGGTTTTCATCAACCGCGACCGAGG
  CTTCGGCTTCATCCGCCTGGAATCCAGAACACTGGCTGAAATTGCAAAAGCAGAGTTGGATGGTACCATA
  CTGAAGAGCAGACCTCTCCGAATTCGCTTTGCTACACATGGAGCAGCCCTAACAGTCAAGAACCTGTCTC
  CAGTTGTTTCCAATGAGCTTCTAGAGCAAGCATTTTCCCAGTTTGGTCCAGTAGAGAAAGCTGTCGTGGT
  TGTGGATGATCGTGGTAGAGCTACAGGAAAAGGTTTTGTAGAATTTGCAGCGAAACCTCCTGCACGAAAG
  GCTCTGGAGAGATGTGGTGATGGGGCGTTCTTGCTAACAACAACACCTCGTCCAGTCATTGTAGAACCCA
  TGGAGCAGTTTGATGATGAAGATGGTTTGCCAGAGAAGCTAATGCAGAAAACTCAACAGTACCATAAGGA
  AAGAGAACAGCCACCACGGTTTGCTCAACCTGGGACATTTGAATTTGAATATGCATCTCGATGGAAGGCT
  CTGGATGAAATGGAGAAGCAGCAGCGTGAACAAGTTGATAGAAACATCAGAGAAGCCAAGGAGAAACTGG
  AGGCAGAGATGGAAGCCGCTAGACATGAACACCAGTTAATGCTAATGAGGCAAGATCTAATGAGACGTCA
  AGAAGAACTCAGACGCTTGGAAGAACTCAGAAATCAAGAACTGCAGAAACGAAAGCAAATACAACTGAGA
  CATGAAGAGGAACATCGTCGTCGTGAAGAAGAAATGATCAGACACAGAGAACAGGAGGAACTGAGGCGAC
  AGCAGGAGGGCTTTAAACCAAACTATATGGAAAATAGAGAACAGGAAATGAGAATGGGTGATATGGGTCC
  CCGTGGAGCAATAAACATGGGAGATGCATTTAGCCCAGCACCTGCTGGTACCCAAGGGCCTCCTCCAATG
  ATGGGTATGAATATGAACAACAGAGGAACTATACCTGGCCCACCAATGGGTCCTGGTCCTGCCATGGGAC
  CAGAAGGAGCTGCAAATATGGGAACTCCAATGATACCAGATAATGGCGCAGTGCACAATGATAGGTTTCC
  CCAAGGTCCTCCATCCCAAATGGGTTCGCCTATGGGAAACAGAACAGGTTCTGAAACCCCCCAGGCACCA
  ATGAGTGCTGTAGGGCCTGTGAGTGGTGGTCCTGGTGGCTTTGGCAGAGGGAGTCAAGGGGGCAATTTTG
  AAGGCCCTAATAAGCGTCGGAGATAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210895 representing NM_001025672
  Red=Cloning site Green=Tags(s)
  
  MMLRGNLKQVRIEKNPARLRALESAAGESEPVAAAAMALTLAGEQPPPPAPSEEHPDEEMGFTIDIKSFL
  KPGEKTYTQRCRLFVGNLPTDITEEDFKRLFERYGEPSEVFINRDRGFGFIRLESRTLAEIAKAELDGTI
  LKSRPLRIRFATHGAALTVKNLSPVVSNELLEQAFSQFGPVEKAVVVVDDRGRATGKGFVEFAAKPPARK
  ALERCGDGAFLLTTTPRPVIVEPMEQFDDEDGLPEKLMQKTQQYHKEREQPPRFAQPGTFEFEYASRWKA
  LDEMEKQQREQVDRNIREAKEKLEAEMEAARHEHQLMLMRQDLMRRQEELRRLEELRNQELQKRKQIQLR
  HEEEHRRREEEMIRHREQEELRRQQEGFKPNYMENREQEMRMGDMGPRGAINMGDAFSPAPAGTQGPPPM
  MGMNMNNRGTIPGPPMGPGPAMGPEGAANMGTPMIPDNGAVHNDRFPQGPPSQMGSPMGNRTGSETPQAP
  MSAVGPVSGGPGGFGRGSQGGNFEGPNKRRRY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001025672
• ORF Size: 1566 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025672.1, NP_001020843.1
• RefSeq Size: 2034 bp
• RefSeq ORF: 1569 bp
• Locus ID: 305910
• Cytogenetics: 15p12
• MW: 58.8 kDa