Yars (NM_001025696) Rat Tagged ORF Clone

CAT#: RR210925

Yars (Myc-DDK-tagged ORF) - Rat tyrosyl-tRNA synthetase (Yars), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Yars
• Synonyms: MGC112837
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210925 representing NM_001025696
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAACCTGACGGGACTAGCGTGACAGTTCCCGGCACGCGGAGACGGCGGCGAACGAGGAAAGGGCGCG
  GGTGCCGGCTGAGCGCGGGAAACCGAGAGAGCGGAGCCATGGGGGATGCTCCAAGCCCTGAGGAGAAGCT
  ACACCTTATCACCCGGAACCTGCAGGAGGTTCTGGGGGAAGAGAAGCTGAAGGAGATACTGAAGGAGCGG
  GAACTTAAAGTTTACTGGGGCACGGCTACCACCGGGAAGCCACACGTGGCCTACTTTGTACCCATGTCCA
  AGATTGCTGACTTCTTGAAGGCAGGGTGTGAGGTAACCATCCTGTTTGCAGACCTTCATGCATACCTGGA
  CAACATGAAAGCTCCCTGGGAACTTCTAGAACTTCGAACCAGTTACTACGAAAATGTGATCAAGGCAATG
  CTGGAGAGTATTGGTGTGCCCTTGGAGAAGCTCAAGTTTACCAAAGGCACCGACTACCAGCTCAGCAAAG
  AGTACACACTGGATGTGTACCGGCTGTCCTCCCTGGTCACACAACATGATGCCAAGAAAGCCGGGGCTGA
  GGTCGTAAAGCAGGTGGAGCACCCTTTGCTGAGTGGTCTGTTGTATCCCGGTCTGCAGGCCTTAGACGAA
  GAGTACTTAAAAGTAGATGCCCAGTTTGGCGGTATTGATCAGAGGAAGATTTTTACCTTTGCAGAGAAGT
  ATCTCCCCACACTTGGCTACTCAAAACGGGTCCATCTGATGAACCCCATGGTTCCTGGGTTAACTGGGAG
  CAAAATGAGCTCATCAGAAGAGGAGTCCAAAATCGACCTCCTTGATCGGAAGGAGGATGTGAAGAAAAAG
  CTGAAGAAGGCCTTCTGTGAGCCCGGCAACGTGGAGAACAATGGGGTGCTGTCCTTCGTCAAGCACGTCC
  TCTTTCCTCTCAAGTCTGAGTTTGTGATCCTGAGAGATGAGAAGTGGGGTGGAAACAAAACCTACACCAT
  TTACCAGGAGCTGGAAAAGGACTTTGCTGCTGAGGTTGTACACCCTGGAGACCTAAAGAATTCTGTTGAA
  GTCGCCCTGAACAAGTTGCTGGACCCCATTCGAGAAAAGTTTAATACCCCAGCCCTGAAGAAGCTGGCCA
  GTGCTGCCTACCCTGATCCTTCAAAGCAGAAGCCTACTGCCAAAGGCCCTGCCAAGAGTTCAGAACCAGA
  GGAGATCATCCCATCCCGGCTGGACATCCGTGTGGGCAAAATCCTCAGCGTGGAAAAGCACCCAGATGCA
  GATAGCCTGTATGTGGAGAAGATTGATGTGGGGGAAGCCGAACCCAGGACTGTGGTGAGCGGCCTCGTGC
  AGTTTGTGCCCAAAGAAGAACTACAGGACCGACTGGTGGTGGTGCTGTGCAACCTGAAACCCCAGAAGAT
  GAGAGGAGTGGACTCCCAGGGCATGCTACTGTGTGCTTCTGTAGAAGGGGTAAGCCGCCAGGTGGAACCT
  CTGGACCCTCCTGCAGGCTCTGCCCCTGGTGAACGGGTATTTGTACAGGGCTATGAGAAGGGCCAACCTG
  ACGAGGAGCTCAAGCCCAAGAAGAAAGTCTTTGAGAAGCTGCAGGCCGACTTTAAGATTTCTGATGACTG
  CGTCGCACAGTGGAAGCAAACCAACTTCATGACCAAGCTGGGCTTTGTCTCCTGTAAATCACTAAAAGGG
  GGCAACATCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210925 representing NM_001025696
  Red=Cloning site Green=Tags(s)
  
  MQPDGTSVTVPGTRRRRRTRKGRGCRLSAGNRESGAMGDAPSPEEKLHLITRNLQEVLGEEKLKEILKER
  ELKVYWGTATTGKPHVAYFVPMSKIADFLKAGCEVTILFADLHAYLDNMKAPWELLELRTSYYENVIKAM
  LESIGVPLEKLKFTKGTDYQLSKEYTLDVYRLSSLVTQHDAKKAGAEVVKQVEHPLLSGLLYPGLQALDE
  EYLKVDAQFGGIDQRKIFTFAEKYLPTLGYSKRVHLMNPMVPGLTGSKMSSSEEESKIDLLDRKEDVKKK
  LKKAFCEPGNVENNGVLSFVKHVLFPLKSEFVILRDEKWGGNKTYTIYQELEKDFAAEVVHPGDLKNSVE
  VALNKLLDPIREKFNTPALKKLASAAYPDPSKQKPTAKGPAKSSEPEEIIPSRLDIRVGKILSVEKHPDA
  DSLYVEKIDVGEAEPRTVVSGLVQFVPKEELQDRLVVVLCNLKPQKMRGVDSQGMLLCASVEGVSRQVEP
  LDPPAGSAPGERVFVQGYEKGQPDEELKPKKKVFEKLQADFKISDDCVAQWKQTNFMTKLGFVSCKSLKG
  GNIS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001025696
• ORF Size: 1692 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025696.1, NP_001020867.2
• RefSeq Size: 2466 bp
• RefSeq ORF: 1695 bp
• Locus ID: 313047
• Cytogenetics: 5q36
• MW: 63 kDa