Hibch (NM_001013112) Rat Tagged ORF Clone

CAT#: RR211069

Hibch (Myc-DDK-tagged ORF) - Rat 3-hydroxyisobutyryl-Coenzyme A hydrolase (Hibch), nuclear gene encoding mitochondrial protein, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hibch
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR211069 representing NM_001013112
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCAGCAGTTTGTGTGGCGGCTCCAGTCGAGGTTCAGCTCAATCAGAAGAGCCAGTGTTATTCTGC
  AACATCTGAGAATGTCCAAGCACACAGAGACTGCAGAAGTGCTGCTGGAGAGGAGAGGCTGTGCAGGGGT
  CATAACGCTCAACAGACCCAAGTTACTCAACGCTCTGAGTCTGAATATGATCCGGCAGATCTATCCGCAG
  CTAAAGAAGTGGGAACGAGACCCAGATACATTCCTGATCATCATAAAGGGAGCTGGAGGAAAGGCCTTCT
  GTGCTGGAGGGGACATCAAAGCACTCTCAGAAGCTAAAAAAGCAGGGCAGACTTTGAGTCAAGATTTATT
  CAGAGAAGAATATATCCTGAACAATGCCATTGCGTCTTGTCAGAAACCGTATGTTGCCCTTATTGATGGA
  ATCACAATGGGTGGGGGAGTTGGTCTTTCAGTCCATGGTCAATTCCGAGTGGCTACAGAAAGATCTCTCT
  TTGCTATGCCAGAAACGGGAATAGGACTGTTTCCTGATGTGGGCGGAGGTTATTTCTTGCCAAGACTTCA
  AGGAAAACTGGGTTACTTCCTTGCACTGACAGGATTCAGACTAAAAGGAAGAGATGTGCACAGAGCGGGA
  ATCGCCACACACTTTGTAGATTCAGAAAAGTTGCATGTACTGGAGGAGGAGTTGTTAGCCTTAAAGTCCC
  CTTCAGCTGAGGATGTTGCTGGTGTCTTAGAAAGCTATCATGCCAAGTCCAAGATGGGTCAAGACAAGTC
  TATCATATTTGAGGAACACATGGACAAAATAAACAGTTGTTTTTCAGCCAATACTGTGGAACAGATTCTT
  GAAAACTTACGGCAAGATGGTTCACCTTTTGCTATGGAACAGATTAAGTTCTCATTGACAAAGACCAGAC
  TCCAAAATGGAAACCAGCTGATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR211069 representing NM_001013112
  Red=Cloning site Green=Tags(s)
  
  MGQQFVWRLQSRFSSIRRASVILQHLRMSKHTETAEVLLERRGCAGVITLNRPKLLNALSLNMIRQIYPQ
  LKKWERDPDTFLIIIKGAGGKAFCAGGDIKALSEAKKAGQTLSQDLFREEYILNNAIASCQKPYVALIDG
  ITMGGGVGLSVHGQFRVATERSLFAMPETGIGLFPDVGGGYFLPRLQGKLGYFLALTGFRLKGRDVHRAG
  IATHFVDSEKLHVLEEELLALKSPSAEDVAGVLESYHAKSKMGQDKSIIFEEHMDKINSCFSANTVEQIL
  ENLRQDGSPFAMEQIKFSLTKTRLQNGNQLI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001013112
• ORF Size: 933 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001013112.1, NP_001013130.1
• RefSeq Size: 1738 bp
• RefSeq ORF: 936 bp
• Locus ID: 301384
• Cytogenetics: 9q22
• MW: 34.5 kDa