Prim1 (NM_001008768) Rat Tagged ORF Clone

CAT#: RR211178

Prim1 (Myc-DDK-tagged ORF) - Rat DNA primase, p49 subunit (Prim1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Prim1
• Synonyms: MGC109113
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR211178 representing NM_001008768
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTTCCGCAGACATATGCTCCAAGTGCTGGACTCTCATGACAATGGCCATGCGCATCATAGATCGGG
  CATTGAAGGAGGACTTTGGATTTAAGCACCGCCTCTGGGTGTATTCAGGAAGGAGAGGTGTACATTGTTG
  GGTCTGTGATGAGTCTGTTCGAAAGCTGTCCTCTGCTGTTCGCTCCGGGATCGTGGAGTACTTGAGTCTT
  ATAAAGGGTGGTCACGATGTTAAAAAGAAGGTTCACCTAAATGAGAAAGTTCACCCTTTTGTCAGAAAAT
  CTATAAACATAATTAAAAAGTACTTTGAAGAATATGCCTTAGTTGACCAAGATATTCTTGAAAATAAAGA
  AAACTGGGATAAGATTTTAGCCCTTGTTCCTGAGACAGTTCATGATGAGCTGCAAAAAGGGTTTCAGAGA
  TTTCACAGTTCACCTCAGCGCTGGGAATACCTGAGGAAAGTGGCCAGTGCACCACAGAATACCAAGAATG
  ATAAGTGTGGCCCCTGGCTAGAATGGGAGATTATGCTCCAGTACTGTTTCCCACGCCTGGATATCAACGT
  CAGCAAAGGAGTCAACCATTTACTGAAGAGCCCTTTTAGTGTTCACCCTAAGACAGGTCGCATTTCTGTG
  CCTATCGATTTTCAGAAAGTGGATCAGTTTGATCCATTTGTTGTTCCAACTATAAGTGCTATCTGCCGGG
  AATTGGATGTGGTTTCCACCACTGAGAAGGAGAAGGAGGAAAATGAAACTGACAGCAGACACAGAGTCAG
  AGATTATAAAAGGACCAGCCTAGCACCATACGTGAAAGTATTTGAACAGTTTCTCGAAAACCTGGATAAA
  TCTCGGAAAGGGGCACTTCTCAAGAAGAGCGATTTACAAAAAGATTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR211178 representing NM_001008768
  Red=Cloning site Green=Tags(s)
  
  MLSADICSKCWTLMTMAMRIIDRALKEDFGFKHRLWVYSGRRGVHCWVCDESVRKLSSAVRSGIVEYLSL
  IKGGHDVKKKVHLNEKVHPFVRKSINIIKKYFEEYALVDQDILENKENWDKILALVPETVHDELQKGFQR
  FHSSPQRWEYLRKVASAPQNTKNDKCGPWLEWEIMLQYCFPRLDINVSKGVNHLLKSPFSVHPKTGRISV
  PIDFQKVDQFDPFVVPTISAICRELDVVSTTEKEKEENETDSRHRVRDYKRTSLAPYVKVFEQFLENLDK
  SRKGALLKKSDLQKDF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001008768
• ORF Size: 888 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001008768.1, NM_001008768.2, NP_001008768.2
• RefSeq Size: 1626 bp
• RefSeq ORF: 891 bp
• Locus ID: 246327
• Cytogenetics: 7q11
• MW: 34.6 kDa
• Gene Summary: human homolog is the catalytic subunit of a primase complex that synthesizes small RNA primers to prime DNA replication [RGD, Feb 2006]