Prim1 (NM_001008768) Rat Untagged Clone

CAT#: RN211178

Prim1 (untagged ORF) - Rat DNA primase, p49 subunit (Prim1), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Prim1
• Synonyms: MGC109113
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN211178 representing NM_001008768
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTTCCGCAGACATATGCTCCAAGTGCTGGACTCTCATGACAATGGCCATGCGCATCATAGATCGGG
  CATTGAAGGAGGACTTTGGATTTAAGCACCGCCTCTGGGTGTATTCAGGAAGGAGAGGTGTACATTGTTG
  GGTCTGTGATGAGTCTGTTCGAAAGCTGTCCTCTGCTGTTCGCTCCGGGATCGTGGAGTACTTGAGTCTT
  ATAAAGGGTGGTCACGATGTTAAAAAGAAGGTTCACCTAAATGAGAAAGTTCACCCTTTTGTCAGAAAAT
  CTATAAACATAATTAAAAAGTACTTTGAAGAATATGCCTTAGTTGACCAAGATATTCTTGAAAATAAAGA
  AAACTGGGATAAGATTTTAGCCCTTGTTCCTGAGACAGTTCATGATGAGCTGCAAAAAGGGTTTCAGAGA
  TTTCACAGTTCACCTCAGCGCTGGGAATACCTGAGGAAAGTGGCCAGTGCACCACAGAATACCAAGAATG
  ATAAGTGTGGCCCCTGGCTAGAATGGGAGATTATGCTCCAGTACTGTTTCCCACGCCTGGATATCAACGT
  CAGCAAAGGAGTCAACCATTTACTGAAGAGCCCTTTTAGTGTTCACCCTAAGACAGGTCGCATTTCTGTG
  CCTATCGATTTTCAGAAAGTGGATCAGTTTGATCCATTTGTTGTTCCAACTATAAGTGCTATCTGCCGGG
  AATTGGATGTGGTTTCCACCACTGAGAAGGAGAAGGAGGAAAATGAAACTGACAGCAGACACAGAGTCAG
  AGATTATAAAAGGACCAGCCTAGCACCATACGTGAAAGTATTTGAACAGTTTCTCGAAAACCTGGATAAA
  TCTCGGAAAGGGGCACTTCTCAAGAAGAGCGATTTACAAAAAGATTTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001008768
• Insert Size: 891 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001008768.2, NP_001008768.2
• RefSeq Size: 1626 bp
• RefSeq ORF: 891 bp
• Locus ID: 246327
• Cytogenetics: 7q11
• Gene Summary: human homolog is the catalytic subunit of a primase complex that synthesizes small RNA primers to prime DNA replication [RGD, Feb 2006]
  Transcript Variant: This variant (2) uses an alternate splice site in the 5' coding region, which results in a frameshift, compared to variant 1. It encodes isoform 2, which has a shorter and distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from genomic sequence data because no single transcript from the reference strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.