Capn1 (NM_019152) Rat Tagged ORF Clone

CAT#: RR211513

Capn1 (Myc-DDK-tagged ORF) - Rat calpain 1 (Capn1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Capn1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR211513 representing NM_019152
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGAGGAGTTAATCACACCTGTGTACTGCACCGGGGTGTCAGCCCAAGTGCAGAAGCAACGGGACA
  AGGAGTTGGGCTTGGGCCGCCATGAAAATGCCATCAAGTACCTGGGCCAGGATTATGAAAATCTTCGTGC
  AAGATGCCTGCAGAACGGGGTCCTCTTCCAAGACGATGCCTTCCCTCCAGTTTCTCATAGCTTGGGCTTC
  AAGGAACTGGGTCCCAATTCCTCTAAAACCTATGGCATCAAGTGGAAGCGTCCTACGGAACTGCTGTCAA
  ACCCCCAGTTCATCGTGGATGGAGCCACCCGAACGGACATCTGCCAGGGAGCACTGGGGGACTGTTGGCT
  CCTGGCTGCCATTGCCTCGCTCACTCTCAACGAGACTATCCTCCACCGAGTGGTTCCCTACGGCCAGAGC
  TTCCAGGAGGGCTATGCTGGCATCTTTCATTTCCAGCTGTGGCAGTTTGGGGAGTGGGTAGATGTGGTTG
  TGGATGATTTGCTACCCACCAAGGATGGAAAGCTGGTGTTCGTGCACTCTGCCCAGGGCAACGAGTTCTG
  GAGCGCTCTCCTGGAGAAAGCCTATGCAAAAGTGAATGGCAGCTATGAGGCCCTTTCGGGAGGCTGCACC
  TCAGAGGCCTTCGAGGACTTTACCGGTGGGGTCACTGAGTGGTACGATCTGCAGAAGGCACCCAGCGACC
  TCTACCAGATCATTCTGAAGGCCCTGGAACGAGGCTCCTTGCTGGGCTGCTCCATTAATATCTCCGATAT
  CCGTGATTTGGAGGCTATTACTTTTAAGAACCTGGTGAGGGGCCATGCATACTCTGTGACGGATGCCAAG
  CAGGTCACTTACCAGGGCCAGCGGGTGAACCTAATCCGGATGCGGAACCCCTGGGGTGAAGTGGAGTGGA
  AAGGACCCTGGAGTGACAACTCCTATGAGTGGAACAAAGTGGACCCCTATGAACGTGAGCAGCTGAGGGT
  CAAGATGGAGGACGGGGAGTTCTGGATGTCCTTCCGGGACTTCATACGTGAGTTCACCAAACTGGAAATC
  TGCAACCTTACACCTGACGCCCTTAAGAGCAGAACCCTCCGGAACTGGAATACCACATTTTACGAGGGCA
  CCTGGCGCCGGGGAAGCACTGCTGGAGGCTGCAGGAACTACCCAGCTACCTTCTGGGTAAACCCCCAGTT
  CAAGATCCGGCTGGAGGAGGTGGATGACGCAGACGACTATGACAGTCGAGAGTCCGGCTGCAGCTTCTTG
  TTGGCCCTCATGCAGAAGCATCGCCGCAGGGAGCGTCGCTTTGGCCGGGACATGGAGACCATTGGTTTTG
  CAGTGTACCAGGTCCCTCGGGAGTTGGCGGGTCAGCCTGTGCACTTGAAGCGTGACTTCTTCCTGGCCAA
  TGCTTCTCGGGCACAATCAGAGCACTTTATCAACCTTCGAGAGGTCAGTAACCGCATCCGCCTGCCGCCG
  GGGGAGTATATAGTGGTGCCCTCCACCTTCGAGCCCAACAAAGAGGGTGACTTTCTGCTGCGCTTCTTTT
  CAGAGAAGAAGGCTGGGACCCAGGAACTAGATGACCAGATCCAGGCCAACCTCCCCGATGAGAAAGTGCT
  CTCTGAAGAGGAGATCGATGACAACTTCAAAACCCTGTTCAGCAAGTTGGCAGGGGATGACATGGAGATC
  AGTGTCAAGGAGTTACAGACCATCTTGAACAGAATCATTAGCAAACACAAAGACCTGCGCACTAACGGCT
  TCAGCCTGGAGTCGTGCCGCAGCATGGTGAACCTCATGGATCGAGACGGTAATGGGAAACTGGGCCTGGT
  GGAGTTCAACATCCTGTGGAACCGCATCCGAAATTACCTGACCATCTTCCGAAAGTTTGACCTGGACAAG
  TCTGGCAGCATGAGTGCTTATGAGATGAGGATGGCCATCGAGGCTGCAGGCTTCAAGCTTAACAAGAAGC
  TGCATGAGCTCATCATCACCCGCTACTCAGAGCCCGACCTGGCCGTGGACTTTGACAACTTTGTGTGCTG
  CCTTGTGCGCCTGGAGACCATGTTCCGGTTTTTCAAAATTCTGGACACAGACCTGGATGGTGTTGTGACC
  TTTGATCTATTTAAGTGGTTACAGCTGACTATGTTTGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR211513 representing NM_019152
  Red=Cloning site Green=Tags(s)
  
  MAEELITPVYCTGVSAQVQKQRDKELGLGRHENAIKYLGQDYENLRARCLQNGVLFQDDAFPPVSHSLGF
  KELGPNSSKTYGIKWKRPTELLSNPQFIVDGATRTDICQGALGDCWLLAAIASLTLNETILHRVVPYGQS
  FQEGYAGIFHFQLWQFGEWVDVVVDDLLPTKDGKLVFVHSAQGNEFWSALLEKAYAKVNGSYEALSGGCT
  SEAFEDFTGGVTEWYDLQKAPSDLYQIILKALERGSLLGCSINISDIRDLEAITFKNLVRGHAYSVTDAK
  QVTYQGQRVNLIRMRNPWGEVEWKGPWSDNSYEWNKVDPYEREQLRVKMEDGEFWMSFRDFIREFTKLEI
  CNLTPDALKSRTLRNWNTTFYEGTWRRGSTAGGCRNYPATFWVNPQFKIRLEEVDDADDYDSRESGCSFL
  LALMQKHRRRERRFGRDMETIGFAVYQVPRELAGQPVHLKRDFFLANASRAQSEHFINLREVSNRIRLPP
  GEYIVVPSTFEPNKEGDFLLRFFSEKKAGTQELDDQIQANLPDEKVLSEEEIDDNFKTLFSKLAGDDMEI
  SVKELQTILNRIISKHKDLRTNGFSLESCRSMVNLMDRDGNGKLGLVEFNILWNRIRNYLTIFRKFDLDK
  SGSMSAYEMRMAIEAAGFKLNKKLHELIITRYSEPDLAVDFDNFVCCLVRLETMFRFFKILDTDLDGVVT
  FDLFKWLQLTMFA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019152
• ORF Size: 2139 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_019152.1, NM_019152.2, NP_062025.1
• RefSeq Size: 3020
• RefSeq ORF: 2142
• Locus ID: 29153
• MW: 82.1 kDa
• Gene Summary: calcium-dependent protease that may be involved in experimental allergic encephalomyelitis (EAE) [RGD, Feb 2006]