Dcx (NM_053379) Rat Tagged ORF Clone

CAT#: RR211526

Dcx (Myc-DDK-tagged ORF) - Rat doublecortin (Dcx), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dcx
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR211526 representing NM_053379
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACTTGATTTTGGACATTTTGACGAACGAGACAAAGCATCTAGAAATATGAGAGGGTCACGGATGA
  ATGGACTTCCAAGTCCCACTCATAGTGCTCACTGTAGCTTCTACAGAACCAGAACCTTGCAGGCATTAAG
  TAATGAGAAGAAGGCCAAGAAGGTACGTTTCTACCGCAATGGGGACCGTTACTTCAAGGGGATTGTGTAC
  GCTGTTTCTTCTGACCGTTTTCGTAGTTTTGATGCATTGCTGGCTGACCTGACTCGATCCTTGTCCGACA
  ACATCAACCTGCCTCAGGGAGTGCGCTACATTTACACTATTGATGGATCCAGAAAGATTGGAAGCATGGA
  TGAACTGGAAGAAGGGGAAAGCTATGTCTGTTCCTCAGACAACTTCTTTAAAAAGGTTGAGTATACCAAG
  AATGTCAATCCCAACTGGTCGGTCAATGTAAAGACATCAGCCAACATGAAAGCGCCCCAGTCCTTGGCTA
  GCAGCAACAGTGCTCAAGCCAGAGAGAACAAGGACTTCGTGCGCCCCAAACTTGTGACCATCATTCGCAG
  TGGGGTGAAGCCTCGGAAGGCGGTGCGTGTGCTTCTCAACAAGAAAACAGCCCACTCTTTCGAGCAGGTC
  CTCACTGACATCACAGAAGCGATCAAACTGGAAACCGGAGTTGTCAAAAAACTCTATACCCTTGATGGAA
  AGCAGGTCACCTGTCTCCATGATTTCTTTGGTGATGATGATGTGTTCATTGCTTGTGGCCCTGAAAAGTT
  CCGCTATGCTCAAGATGACTTCTCCTTGGATGAGAATGAATGCAGAGTCATGAAAGGGAATCCATCTGCC
  ACAGCTGGCCCAAAAGCTTCCCCAACACCTCAAAAGACATCTGCTAAAAGCCCAGGCCCAATGCGCCGCA
  GCAAGTCTCCAGCTGACTCAGGTAACGACCAAGACGCAAATGGAACTTCTAGCAGTCAGCTCTCAACACC
  TAAGTCGAAGCAGTCTCCTATCTCTACACCCACAAGCCCTGGAAGTCTGCGGAAGCACAAGGACTTGTAC
  CTGCCACTGTCATTGGATGACTCTGATTCACTTGGCGATTCCATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR211526 representing NM_053379
  Red=Cloning site Green=Tags(s)
  
  MELDFGHFDERDKASRNMRGSRMNGLPSPTHSAHCSFYRTRTLQALSNEKKAKKVRFYRNGDRYFKGIVY
  AVSSDRFRSFDALLADLTRSLSDNINLPQGVRYIYTIDGSRKIGSMDELEEGESYVCSSDNFFKKVEYTK
  NVNPNWSVNVKTSANMKAPQSLASSNSAQARENKDFVRPKLVTIIRSGVKPRKAVRVLLNKKTAHSFEQV
  LTDITEAIKLETGVVKKLYTLDGKQVTCLHDFFGDDDVFIACGPEKFRYAQDDFSLDENECRVMKGNPSA
  TAGPKASPTPQKTSAKSPGPMRRSKSPADSGNDQDANGTSSSQLSTPKSKQSPISTPTSPGSLRKHKDLY
  LPLSLDDSDSLGDSM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053379
• ORF Size: 1095 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_053379.1, NM_053379.2, NM_053379.3, NP_445831.3
• RefSeq Size: 8827
• RefSeq ORF: 1098
• Locus ID: 84394
• MW: 40.5 kDa
• Gene Summary: This gene encodes a member of the doublecortin family. The protein encoded by this gene contains two doublecortin domains, which bind microtubules and regulate microtubule polymerization. In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The encoded protein appears to direct neuronal migration by regulating the organization and stability of microtubules. Studies in knockout mice lacking this gene suggest that this gene has a cortical role in nuclear translocation and positioning of the mitotic spindle in radial glial mitotic division. This gene is essential for neuronal migration, differentiation, and plasticity, is required for hippocampal development and also plays a role in dendrites development. [provided by RefSeq, Sep 2010]