Tle2 (NM_001039013) Rat Tagged ORF Clone

CAT#: RR211577

Tle2 (Myc-DDK-tagged ORF) - Rat transducin-like enhancer of split 2 (E(sp1) homolog, Drosophila) (Tle2), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tle2
• Synonyms: MGC124808
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR211577 representing NM_001039013
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACCCCCAGGGAAGGCACCCGACCCCGCTGCAGTCTGGCCAGCCTTTCAAGTTCTCAGTCCTGGAAA
  TCTGTGACCGGATCAAAGAGGAATTTCAGTTTCTCCAAGCTCAATACCACAGCCTTAAGCTAGAGTGTGA
  GAAGCTGGCCAGCGAGAAGACAGAAATGCAAAGGCATTATGTGATGTACTATGAGATGTCCTACGGACTC
  AACATTGAGATGCACAAACAGGCTGAGATCGTGAAGCGCCTCAGTGCAATCTGTGCCCAGATGGTCCCAT
  TCCTCACTCAGGAGCATCAGCAGCAGGTGCTCCAGGCTGTGGACCGAGCCAAGCAGGTGACCGTGGGGGA
  ACTGAACAACCTCTTGGGGCAGCAGAATCAGCTCCAGCCGCTGTCCCATGCACCCCCTGTGCCTCTCACC
  CCACGCCCAGCTGGCCTGGTGGGTGCCGGGGCCACTGGGCTGCTGGCCCTCTCTGGGGCGCTGGCTGCGC
  AGGCCCAGCTGGTGGCTGCTGTTAAGGAAGACCGTGTGGGTGTGGACACCGAGGGGTCCAGAGTGGACAG
  AGCTGCCAGCAGGAGTTCATCCCCCTCACCCCCTGAGAGTCTGGCGGAAGAGGAGCATCCTAGCAGCCGA
  GGCAGTAACGGGAAGCAGCAGAGAGCCGAAGACAAGAATATGTCAGGGCCTTATGACAGTGAGGAAGACA
  AGAGTGACTATAACCTGGTAGTGGATGAGGACCAACCGTCAGAGCCCCCCAGCCCTGCGACCACCCCCTG
  TGGGAAGGCGCCCCTCTGCATCCCTGCCCGCAGGGACCTCACGGACAGTCCAGCCTCCCTGGCTTCCAGC
  TTGGGCTCACCACTCCCCAGAAGCAAAGACCTAGCCCTGAACGATCTCTCCACAGGTACTCCCGCCTCTG
  GGTCATGTGGTCCCTCTCCACCCCAGGACTCGTCCACCCCAGGGCCCAGCTCAGCCAGTCACCTCTGCCA
  GCTGGCTACTCAGCCGGCACCGCCCACAGACAGCATTGCCCTGAGGAGTCCCCTGACCCTGTCCAGCCCT
  TTCACCTCGTCCTTCAGCCTGGGCTCCCACAGCACCCTCAATGGGGACCTCTCCATGCCTGGCTCCTATG
  TCAGCCTCCACCTGTCCCCCCAGATGCAACCGGTGCCCTTCCCATCGGATGCACTGGTAGGCACTGGCAT
  CCCTCGCCACGCGAGGCAGCTGCACACACTGGCCCATGGTGAGGTGGTGTGTGCCGTCACCATCAGCAGC
  TCCACGCAGCACGTGTACACTGGCGGCAAAGGCTGCGTGAAGGTTTGGGACGTGGGCCAGCCGGGCAGCA
  AGACACCTGTGGCACAGCTGGATTGCCTGAACCGGGACAACTACATTCGCTCCTGCAAGCTGCTGCCTGA
  CGGGCAGAGCCTGATCGTGGGTGGCGAGGCCAGTACCCTGTCCATTTGGGACCTGGCAGCACCCACACCA
  CGCATCAAAGCAGAGCTGACCTCGTCCGCCCCCGCCTGTTACGCGCTGGCCATCAGCCCGGATGCCAAGG
  TCTGCTTCTCCTGCTGCAGCGACGGTAACATCGTGGTCTGGGACCTGCAGAACCAGGCCATGGTCAGACA
  GTTCCAGGGCCACACGGACGGGGCCAGCTGCATTGACATATCAGACTACGGGACCCGGCTGTGGACTGGG
  GGCCTGGACAACACCGTGCGCTGCTGGGATCTGCGCGAGGGCCGCCAGCTGCAGCAACATGACTTCAGTT
  CCCAGATCTTCTCGTTGGGCCACTGTCCCAGTCAGGACTGGCTGGCCGTGGGGATGGAAAGCAGCCACGT
  GGAGGTCCTGCATGTGCGCAAGCCTGAGAAGTACCAGCTCCGCCTGCATGAGAGCTGCGTGCTGTCGCTC
  AAGTTCGCTTCCTGCGGACGCTGGTTTGTGAGCACAGGCAAGGATAACCTGCTCAATGCCTGGAGGACAC
  CCTATGGGGCCAGCATTTTCCAGTCCAAAGAGTCTTCTTCTGTGTTGAGCTGCGACATCTCCAGGAACAA
  CAAGTACATTGTGACAGGCTCAGGGGACAAGAAGGCCACTGTGTATGAGGTGGTGTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR211577 representing NM_001039013
  Red=Cloning site Green=Tags(s)
  
  MYPQGRHPTPLQSGQPFKFSVLEICDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHYVMYYEMSYGL
  NIEMHKQAEIVKRLSAICAQMVPFLTQEHQQQVLQAVDRAKQVTVGELNNLLGQQNQLQPLSHAPPVPLT
  PRPAGLVGAGATGLLALSGALAAQAQLVAAVKEDRVGVDTEGSRVDRAASRSSSPSPPESLAEEEHPSSR
  GSNGKQQRAEDKNMSGPYDSEEDKSDYNLVVDEDQPSEPPSPATTPCGKAPLCIPARRDLTDSPASLASS
  LGSPLPRSKDLALNDLSTGTPASGSCGPSPPQDSSTPGPSSASHLCQLATQPAPPTDSIALRSPLTLSSP
  FTSSFSLGSHSTLNGDLSMPGSYVSLHLSPQMQPVPFPSDALVGTGIPRHARQLHTLAHGEVVCAVTISS
  STQHVYTGGKGCVKVWDVGQPGSKTPVAQLDCLNRDNYIRSCKLLPDGQSLIVGGEASTLSIWDLAAPTP
  RIKAELTSSAPACYALAISPDAKVCFSCCSDGNIVVWDLQNQAMVRQFQGHTDGASCIDISDYGTRLWTG
  GLDNTVRCWDLREGRQLQQHDFSSQIFSLGHCPSQDWLAVGMESSHVEVLHVRKPEKYQLRLHESCVLSL
  KFASCGRWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISRNNKYIVTGSGDKKATVYEVVY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001039013
• ORF Size: 2088 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001039013.1, NP_001034102.1
• RefSeq Size: 2431 bp
• RefSeq ORF: 2091 bp
• Locus ID: 299636
• Cytogenetics: 7q11
• MW: 75.1 kDa