Rufy1 (NM_001100727) Rat Tagged ORF Clone

CAT#: RR211693

Rufy1 (Myc-DDK-tagged ORF) - Rat RUN and FYVE domain containing 1 (Rufy1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rufy1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR211693 representing NM_001100727
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCCCGCGGAGACTGCCGCCGGGCGGGGCAGGATTCGGAGCCGGAGCCGAAGCCGGAGCCCGAGT
  CGCGGCCGGAGCCTGGGCTCCAAGCAGGCCTAGAGTCCGGGGAAGAGTTCGAGATCGTGGACCGGAGCCA
  GCTGCCAAGCCCCGGCGAGCTGCGGAGCGCGTCGCGGACTCGAGCGGCCGAAAGCTGGTCGGCGCCCATC
  CTGACGCTGGCGCGCAGAGCCACCGGGAACCTGTCGGCGAGCTGCGGCAGCGCGCTGCGCGCGGCCGCGG
  GGCTGGGAGACGGCGGCGGCGGCGGCGGGGAGCGCGCAGCTTCCAAAGGCCAGATGATGGAGGAGCGTGC
  CAACCTAATGCACATGATGAAACTCAGCATTAAGGTCTTACTCCAGTCTGCCCTGAGCCTGGGACGCAGC
  CTGGATGCAGACCATGCTCCCTTACAGCAGTTCTTTGTAGTGATGGAGCATTGCCTCAAGCATGGGCTAA
  AAGTCAAGAAGAGTTTCATTGGCCAAAATAAATCTTTCTTTGGTCCTTTGGAGCTGGTGGAAAAACTTTG
  CCCTGAAGCATCAGATATAGCTACCAGTGTCAGGAATCTTCCAGAACTAAAGACAGCCGTGGGAAGAGGC
  AGAGCGTGGCTTTATCTTGCTCTCATGCAAAAGAAACTGGCAGATTATCTGAAAGTCCTCATAGACAACA
  AGCATCTCCTAAGTGAGTTCTATGAGCCTGAGGCCTTAATGATGGAGGAGGAAGGAATGGTGATTGTCGG
  TCTGCTGGTGGGGCTCAACGTTCTGGACGCCAATCTCTGCTTAAAGGGAGAAGACCTGGATTCTCAGGTC
  GGAGTGATTGACTTTTCCCTCTATCTGAAGGACGTGCAGGATCTTGACAGCGGCAGAGAGCATGAAAGAA
  TTACCGATGTCCTTGATCAGAAAAATTACGTGGAGGAACTTAACCGGCACTTAAGCTGCACAGTTGGGGA
  TCTTCAAACCAAGATAGATGGCTTGGAAAAGACGAACTCAAAGCTTCAAGAAGAGCTTTCAGCTGCAACA
  GACCGAATTTGTTCCCTACAAGAAGAACAGCAGCAGCTAAGAGAACAGAATGAATTAATTCGAGAAAGAA
  GCGAGAAGAGTGTGGAGATAACAAAACAGGACACCAAAGTTGAACTGGAGACTTATAAGCAGACCCGGCA
  GGGCCTGGATGAAATGTACAGCGATGTGTGGAAACAGCTGAAGGAGGAGAAGAAAGTCCGACTGGAACTA
  GAAAAGGAACTGGAGTTACAGATTGGAATGAAGACCGAGATGGAAATCGCCATGAAGTTACTGGAGAAGG
  ACACCCATGAGAAGCAGGACACACTCGTTGCCCTCCGCCAGCAGCTGGAAGAAGTCAAAGCAATTAATTT
  ACAGATGTTTCACAAAGTTCAGAGTGCAGAGAGCAGTTTACAGCAGAAGGATGAAGCCATTGCATCTTTT
  GAAGGAAAAACCACCCAAGTGATGTCCAGCATGAAACAAATGGAGGAAAGGTTACAGCAGGCTGAGCGGG
  CCAGGCAAGGGGCAGAAGAGAGGAGCTACAAGCTGCAGCAGGAGCTCAGCGGGAGGGTCAGTGCCCTGCA
  GCTGCAGCTGTCCCAGCTGCGGGACCAATGCTCAGGTCTAGAGAAAGAACTGAAGTCAGAAAAAGAACAA
  AGACAAACTCTTCAGAGAGAACTACAGCATGAGAAAGACACCTCCTGTCTGCTGCAGACAGAGCTGCAGC
  AAGTGGAAGGCCTGAAGAAGGAGCTTCGGGAGCTCCAAGACGAGAAGGCAGAGTTGCGGAAGGTCTGTGA
  GGAACAGGAGCAAGCCCTCCAGGAAATGGGACTGCACCTTAGCCAGTCCAAACTGAAGATGGAGGACATC
  AAGGAAGTAAACAAGGCTCTGAAGGGCCACACATGGTTGAAAGATGATGAGGCAACACATTGTAAGCAGT
  GTGAGAAGGACTTCTCCATTTCCCGGAGGAAGCACCACTGCCGGAACTGTGGCCACATTTTCTGCAACAC
  CTGCTCTAGCAACGAGCTGGCTCTGCCCTCCTACCCTAAGCCAGTGCGTGTGTGTGACAGCTGCCATACC
  CTGCTACTGCAGCGCTGCTCTTCCACAGCCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR211693 representing NM_001100727
  Red=Cloning site Green=Tags(s)
  
  MAARGDCRRAGQDSEPEPKPEPESRPEPGLQAGLESGEEFEIVDRSQLPSPGELRSASRTRAAESWSAPI
  LTLARRATGNLSASCGSALRAAAGLGDGGGGGGERAASKGQMMEERANLMHMMKLSIKVLLQSALSLGRS
  LDADHAPLQQFFVVMEHCLKHGLKVKKSFIGQNKSFFGPLELVEKLCPEASDIATSVRNLPELKTAVGRG
  RAWLYLALMQKKLADYLKVLIDNKHLLSEFYEPEALMMEEEGMVIVGLLVGLNVLDANLCLKGEDLDSQV
  GVIDFSLYLKDVQDLDSGREHERITDVLDQKNYVEELNRHLSCTVGDLQTKIDGLEKTNSKLQEELSAAT
  DRICSLQEEQQQLREQNELIRERSEKSVEITKQDTKVELETYKQTRQGLDEMYSDVWKQLKEEKKVRLEL
  EKELELQIGMKTEMEIAMKLLEKDTHEKQDTLVALRQQLEEVKAINLQMFHKVQSAESSLQQKDEAIASF
  EGKTTQVMSSMKQMEERLQQAERARQGAEERSYKLQQELSGRVSALQLQLSQLRDQCSGLEKELKSEKEQ
  RQTLQRELQHEKDTSCLLQTELQQVEGLKKELRELQDEKAELRKVCEEQEQALQEMGLHLSQSKLKMEDI
  KEVNKALKGHTWLKDDEATHCKQCEKDFSISRRKHHCRNCGHIFCNTCSSNELALPSYPKPVRVCDSCHT
  LLLQRCSSTAS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001100727
• ORF Size: 2133 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001100727.1, NP_001094197.1
• RefSeq Size: 2666
• RefSeq ORF: 2136
• Locus ID: 360521
• MW: 80.4 kDa