Pygb (NM_013188) Rat Tagged ORF Clone

CAT#: RR211838

Pygb (Myc-DDK-tagged ORF) - Rat phosphorylase, glycogen, brain (Pygb), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pygb
• Synonyms: GLYPHOA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR211838 representing NM_013188
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAAGCCATTGACGGACAGTGAGAGGCAGAAGCAGATCAGTGTGCGTGGCATCGCCGGGCTAGGCG
  ACGTGGCCGAGGTGCGGAAGAGCTTCAACAGGCACCTGCACTTCACGCTGGTCAAGGACCGCAATGTGGC
  CACTCCCCGCGACTACTTCTTCGCTCTGGCACACACGGTTCGCGACCACCTAGTGGGCCGCTGGATCCGC
  ACGCAACAGCATTACTACGAACGGGACCCCAAGCGTATCTACTACCTTTCCCTGGAATTCTACATGGGCC
  GCACACTGCAGAACACCATGGTGAACCTGGGCCTTCAGACTGCATGTGATGAAGCCACTTATCAGTTGGG
  GTTGGACTTGGAAGAACTAGAGGAAATAGAGGAGGATGCTGGCCTTGGGAATGGTGGCCTGGGGCGGCTG
  GCAGCTTGCTTCCTTGATTCGATGGCCACGCTGGGTCTAGCTGCATATGGCTATGGAATCCGCTATGAAT
  TTGGGATTTTCAACCAAAAGATTGTCAATGGCTGGCAGGTTGAAGAAGCTGACGACTGGCTGCGCTACGG
  AAACCCATGGGAGAAGGCGCGGCCGGAGTACATGCTGCCTGTGCACTTCTACGGGAGGGTGGAGCACACC
  CCTAACGGTGTCCTCTGGCTGGACACACAGGTGGTACTCGCCATGCCCTATGACACCCCAGTGCCTGGGT
  ATAAGAACAACACCGTCAACACCATGAGGCTCTGGTCTGCCAAGGCACCCAATGACTTCAAGCTGAAGGA
  CTTCAACGTTGGGGACTACATCGAAGCTGTCCTGGACAGGAACTTGGCCGAGAACATCTCCAGGGTCCTG
  TATCCCAATGACAATTTCTTCGAGGGAAAGGAGCTGCGGCTGAAGCAGGAGTACTTTGTGGTGGCCGCCA
  CCCTCCAGGACATCATTAGAAGGTTCAAGTCGTCCAAGTTTGGCTGCCGGGATCCAGTGAGAACCTGTTT
  CGAGACATTCCCAGACAAGGTAGCCATCCAGCTGAATGACACCCATCCCGCGCTGTCCATCCCCGAGCTC
  ATGCGGATCCTGGTGGACGTGGAGAAGGTAGACTGGGATAAGGCCTGGGAGATCACCAAGAAGACCTGTG
  CCTACACCAACCACACTGTGCTGCCCGAGGCCTTGGAGCGCTGGCCCGTGTCTATGTTTGAGAAGCTGCT
  GCCGCGACACCTGGAAATCATCTATGCCATCAACCAGAGGCACCTGGATCACGTGGCTGCCCTGTTTCCC
  GGGGATGTGGACCGCTTGAGGAGGATGTCTGTGATTGAGGAAGGGGACTGCAAGAGAATCAACATGGCAC
  ACCTGTGTGTGATTGGGTCCCACGCCGTGAACGGGGTGGCAAGGATTCACTCCGAGATCGTGAAGCAGTC
  TGTCTTCAAGGACTTTTATGAGCTGGAGCCAGAGAAGTTCCAGAACAAGACCAATGGTATCACGCCCCGA
  CGGTGGCTGCTCTTGTGCAATCCGGGGCTGGCAGAAATCATTGTGGAGAGAATCGGGGAAGGTTTCCTGA
  CTGATCTGAGCCAACTGAAGAAGCTGCTGTCACTGGTTGATGATGAGGCCTTCATCAGGGATGTAGCCAA
  GGTCAAGCAGGAAAACAAGCTGAAGTTCTCTGCCCAGCTAGAGAAGGAATACAAGGTGAAGATCAACCCT
  GCGTCCATGTTTGATGTGCACGTCAAGAGGATCCACGAGTACAAGCGGCAGCTGCTCAACTGCTTACACA
  TCATCACCCTGTACAACCGAATAAAGAAGGATCCAACCAAGACCTTTGTGCCCAGGACTGTTATGATCGG
  AGGCAAGGCAGCTCCTGGTTACCACATGGCTAAGATGATCATCAAGCTGGTCACATCCATTGGAGATGTT
  GTCAATCATGATCCAGTTGTGGGCGACAGGCTCAGAGTGATCTTCCTGGAGAACTACCGTGTGTCTTTAG
  CTGAGAAAGTGATCCCAGCCGCCGACCTGTCACAGCAGATCTCCACTGCGGGCACCGAGGCCTCGGGCAC
  CGGCAACATGAAGTTCATGCTCAACGGAGCTCTCACCATCGGCACCATGGATGGTGCCAACGTGGAGATG
  GCCGAGGAGGCCGGGGAGGAGAACCTCTTCATCTTTGGTATGCGGGTGGAAGATGTTGAGGCCTTGGACC
  AGAAAGGGTACAATGCCAGAGAATTCTACGAGCGCCTGCCTGAGCTGAGGCAGGCTGTGGATCAGATCAG
  CAGTGGCTTCTTTTCTCCCAAAGATCCAGACTGCTTCAAGGATGTGGTTAACATGCTGATGTACCATGAC
  AGATTCAAGGTATTTGCAGACTATGAAGCCTACATCCAATGCCAGGCCCAGGTGGACCATCTATACCGGA
  ATCCCAAGGATTGGACTAAAAAGGTGATCAGAAATATAGCCTGCTCTGGCAAATTCTCCAGTGACCGGAC
  CATCACTGAGTATGCCCGGGAGATCTGGGGTGTAGAGCCCTCTGACCTGCAGATCCCACCCCCTAATCTC
  CCCAAGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR211838 representing NM_013188
  Red=Cloning site Green=Tags(s)
  
  MAKPLTDSERQKQISVRGIAGLGDVAEVRKSFNRHLHFTLVKDRNVATPRDYFFALAHTVRDHLVGRWIR
  TQQHYYERDPKRIYYLSLEFYMGRTLQNTMVNLGLQTACDEATYQLGLDLEELEEIEEDAGLGNGGLGRL
  AACFLDSMATLGLAAYGYGIRYEFGIFNQKIVNGWQVEEADDWLRYGNPWEKARPEYMLPVHFYGRVEHT
  PNGVLWLDTQVVLAMPYDTPVPGYKNNTVNTMRLWSAKAPNDFKLKDFNVGDYIEAVLDRNLAENISRVL
  YPNDNFFEGKELRLKQEYFVVAATLQDIIRRFKSSKFGCRDPVRTCFETFPDKVAIQLNDTHPALSIPEL
  MRILVDVEKVDWDKAWEITKKTCAYTNHTVLPEALERWPVSMFEKLLPRHLEIIYAINQRHLDHVAALFP
  GDVDRLRRMSVIEEGDCKRINMAHLCVIGSHAVNGVARIHSEIVKQSVFKDFYELEPEKFQNKTNGITPR
  RWLLLCNPGLAEIIVERIGEGFLTDLSQLKKLLSLVDDEAFIRDVAKVKQENKLKFSAQLEKEYKVKINP
  ASMFDVHVKRIHEYKRQLLNCLHIITLYNRIKKDPTKTFVPRTVMIGGKAAPGYHMAKMIIKLVTSIGDV
  VNHDPVVGDRLRVIFLENYRVSLAEKVIPAADLSQQISTAGTEASGTGNMKFMLNGALTIGTMDGANVEM
  AEEAGEENLFIFGMRVEDVEALDQKGYNAREFYERLPELRQAVDQISSGFFSPKDPDCFKDVVNMLMYHD
  RFKVFADYEAYIQCQAQVDHLYRNPKDWTKKVIRNIACSGKFSSDRTITEYAREIWGVEPSDLQIPPPNL
  PKD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013188
• ORF Size: 2529 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_013188.1, NP_037320.1
• RefSeq Size: 3873
• RefSeq ORF: 2532
• Locus ID: 25739
• MW: 96.7 kDa
• Gene Summary: brain specific isozyme of glycogen phosphorylase; poorly activated by phosphorylation [RGD, Feb 2006]