Zap70 (NM_001012002) Rat Tagged ORF Clone

CAT#: RR212247

Zap70 (Myc-DDK-tagged ORF) - Rat zeta-chain (TCR) associated protein kinase (Zap70), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Zap70
• Synonyms: Srk
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR212247 representing NM_001012002
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCGACCCCGCGGCGCATCTGCCATTCTTCTACGGAAGCATTTCGCGGGCCGAGGCCGAGGAGCACC
  TGAAGCTGGCAGGCATGGCCGACGGACTGTTCCTCCTGCGCCAGTGCCTGCGCTCGCTGGGCGGCTACGT
  GCTGTCGCTAGTACACGACGTGCGCTTCCACCATTTCCCCATCGAGCGCCAACTCAATGGCACGTACGCC
  ATCGCGGGCGGCAAGGCGCACTGCGGCCCGGCCGAGCTCTGCCAGTTCTACTCGCAGGACCCCGACGGAC
  TGCCCTGCAATCTGCGTAAGCCATGCAACCGGCCGCCGGGACTTGAGCCTCAGCCCGGGGTCTTCGACTG
  CCTGCGCGATGCTATGGTGCGGGACTACGTGCGCCAGACCTGGAAACTGGAGGGTGATGCACTAGAGCAG
  GCCATCATCAGCCAGGCCCCACAGGTGGAGAAGCTCATTGCTACCACAGCCCACGAGCGAATGCCCTGGT
  ACCACAGCAGCCTGACTCGCGAGGAGGCTGAGCGCAAACTCTATTCTGGCCAGCAGACCGACGGCAAGTT
  CCTGCTGAGACCCCGGAAGGAGCAGGGCACATATGCGCTGTCCCTGGTCTACGGGAAAACTGTGTACCAC
  TATCTCATCAGCCAGGACAAGGCTGGCAAGTACTGTATCCCCGAAGGCACCAAGTTTGATACGCTCTGGC
  AGCTGGTGGAGTACCTGAAGCTGAAGGCAGATGGACTCATTTATCGCCTGAAGGAGGTCTGTCCCAACAG
  CAGTGCCAGCGCCGAAGCTGCTGCACCCACGCTCCCAGCCCACCCATCCACATTCACTCAGCCTCACAGA
  CGAATTGATACCCTGAACTCAGACGGATACACCCCTGAACCAGCACGCTTAGACAAGCCACGACCGATGC
  CCATGGACACGAGCGTGTACGAGAGCCCCTACAGCGACCCCGAGGAACTCAAGGACAAGAAGCTCTTCCT
  GAAGCGAGAGAACCTCCTCGTGGCCGACATCGAACTTGGTTGCGGCAACTTTGGCTCAGTGCGCCAGGGA
  GTCTACCGCATGCGCAAGAAGCAGATCGACGTGGCCATCAAGGTGCTAAAGCAGAGCACAGAGAAAGCTG
  ACAAGGATGAGATGATGCGGGAGGCCCAGATCATGCATCAGCTCGACAACCCCTACATTGTGCGGCTCAT
  CGGCGTGTGCCAGGCAGAGGCGCTCATGCTGGTCATGGAGATGGCAGGAGGCGGGCCACTGCACAAGTTC
  CTGATTGGAAAAAAGGAGGAGATCCCGGTGAGCAACGTGGCTGAACTGCTGCACCAGGTGGCCATGGGCA
  TGAAGTATTTGGAGGAGAAGAACTTTGTGCACCGTGACCTGGCAGCCCGGAATGTTCTACTGGTCAATCG
  GCACTATGCCAAGATCAGCGACTTTGGCCTGTCCAAGGCACTGGGTGCTGATGACAGCTATTACACAGCC
  CGTTCTGCAGGGAAGTGGCCTCTGAAGTGGTACGCACCAGAATGCATCAACTTCCGGAAGTTCTCCAGCC
  GCAGTGATGTCTGGAGCTATGGGGTCACCATGTGGGAGGCCTTCTCCTATGGCCAGAAGCCCTACAAGAA
  GATGAAGGGCCCTGAGGTCCTGGATTTCATCAAGCAGGGTAAGAGGATGGAATGCCCTCCAGAGTGTCCG
  CCCGAAATGTACGCGCTTATGAGTGACTGCTGGATATACAAGTGGGAGGATCGTCCCGATTTCGTGGCTG
  TGGAACAACGAATGAGGACCTATTACTACAGCATGGCTAGCCGGGCAGAGGGACCCCCACAGTGTGAACA
  GGTGGCCGAGGCTGCATGTGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR212247 representing NM_001012002
  Red=Cloning site Green=Tags(s)
  
  MPDPAAHLPFFYGSISRAEAEEHLKLAGMADGLFLLRQCLRSLGGYVLSLVHDVRFHHFPIERQLNGTYA
  IAGGKAHCGPAELCQFYSQDPDGLPCNLRKPCNRPPGLEPQPGVFDCLRDAMVRDYVRQTWKLEGDALEQ
  AIISQAPQVEKLIATTAHERMPWYHSSLTREEAERKLYSGQQTDGKFLLRPRKEQGTYALSLVYGKTVYH
  YLISQDKAGKYCIPEGTKFDTLWQLVEYLKLKADGLIYRLKEVCPNSSASAEAAAPTLPAHPSTFTQPHR
  RIDTLNSDGYTPEPARLDKPRPMPMDTSVYESPYSDPEELKDKKLFLKRENLLVADIELGCGNFGSVRQG
  VYRMRKKQIDVAIKVLKQSTEKADKDEMMREAQIMHQLDNPYIVRLIGVCQAEALMLVMEMAGGGPLHKF
  LIGKKEEIPVSNVAELLHQVAMGMKYLEEKNFVHRDLAARNVLLVNRHYAKISDFGLSKALGADDSYYTA
  RSAGKWPLKWYAPECINFRKFSSRSDVWSYGVTMWEAFSYGQKPYKKMKGPEVLDFIKQGKRMECPPECP
  PEMYALMSDCWIYKWEDRPDFVAVEQRMRTYYYSMASRAEGPPQCEQVAEAACG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001012002
• ORF Size: 1842 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012002.1, NP_001012002.1
• RefSeq Size: 2277 bp
• RefSeq ORF: 1845 bp
• Locus ID: 301348
• Cytogenetics: 9q21
• MW: 69.8 kDa