Dpp10 (NM_001012205) Rat Tagged ORF Clone

CAT#: RR212272

Dpp10 (Myc-DDK-tagged ORF) - Rat dipeptidylpeptidase 10 (Dpp10), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dpp10
• Synonyms: DPP X; DPPY
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR212272 representing NM_001012205
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCAAACTGCCAGCGTGTCCCATCACATCAAGTGTCAGCCCTCCAAAACCATCAAGGAACTGGGAA
  GTAACAGCCCTCCCCAAAGAAACTGGAAAGGAATTGCCATTGCTCTACTGGTGATCTTGGTAGTATGCTC
  CCTCATCACAATGTCTGTCATCCTGTTAACACCAGATGAGCTAACAAATTCTTCAGAAACAAGACTGTCA
  CTAGAGGAGCTTTTGGGAAAAGGGTTTGGACTTCATAATCCAGAAGCTCGATGGATCAATGATACAGATG
  TGGTATATAAAACCGACAATGGGCACGTCATGAAGCTGAATGCAGAAACAAACGCTACCACGTTGTTATT
  GGACAACTCAACTTTCGTAACTTTTAAAGCATCCAGACACTCACTTTCCCCAGATTTAAAATATGTTCTC
  CTGGCATACGACGTCAAGCAGATTTTTCATTACTCATTTACCGCTTCATATTTGATTTACAACATACACA
  CTGGGGAAGTGTGGGAGTTGAATCCCCCAGAAGTTGAGGACTCAGTGTTGCAGTATGCGGCCTGGGGTGT
  GCAAGGACAGCAGCTGATTTATATTTTTGAAAACAATATCTACTACCAACCTGATATCAAGAGCAGTTCT
  TTAAGACTGACATCTTCAGGAAAAGAAGGAATTGTTTTTAATGGGATTGCTGACTGGTTATATGAAGAGG
  AACTTCTGCATTCTCATATTGCTCACTGGTGGTCACCCGACGGAGAGAGACTTGCCTTCCTGATGATAAA
  TGACTCCTTGGTGCCTAACATGGTTATACCTCGGTTTACTGGAGCACTATACCCCAAAGCAAAGCAGTAC
  CCATACCCCAAGGCAGGTCAAGCGAATCCATCAGTGAAGTTATATGTTGTAAACCTGTATGGACCAACTC
  ATACTTTGGAGCTGATGCCACCCGACATTTTTAAATCAAGAGAATATTACATCACCATGGTTAAATGGGT
  GAGCAACACCAGGACAGTGGTCAGATGGTTGAATCGTCCTCAGAACATCTCCATCCTCACAGTCTGTGAG
  TCTACCACCGGGGCATGCAGTAGGAAATACGAAATGACATCTGACACCTGGATCTCTAAACAGAACGAGG
  AGCCAGTGTTTTCTAGAGATGGAAGCAAGTTCTTCATGACTGTTCCTGTCAAACAAGGTGGAAGAGGAGA
  ATTCCACCACATAGCTATGTTCCTGGTCCAGAGTAAAAGCGAGCAAATTACGGTGCGACATCTGACATCA
  GGAAACTGGGAAGTGATAAGGATCTTGGCCTATGATGAAACAACTCAAAAAATCTACTTTCTGAGCACAG
  AATTTTCTCCCCGAGGGAGACAGCTATACAGTGCTTCTACTGAAGGACTATTGAGTCGTGATTGCATCTC
  ATGCAACTTTAGGAAAGAAGATTGCACGTATTTTGATGCCAGCTTTAGTCCCATGAATCAACATTTCTTG
  TTATTCTGTGAAGGTCCAAAGGTCCCAATGGTAAGCCTTCACAGCACAGACAACCCATCAAATTATTACA
  TCTTGGAAAGAAATTCTATGATGAAAGAAACTATTCAGAAGAAGAAGCTCGCAAAGAGAGAGATTAGAAT
  ACTTCACATTGATGACTACGAACTTCCTTTACAGTTGTCCTTTCCTAAAGACTTTTTGGAGAAAAACCAG
  TATGCTCTTCTACTAATAATAGATGAAGAACCAGGAGGCCAGATGGTGACAGAAAAATTTCATGTTGACT
  GGGATTCAGTTCTTATTGACACCGATAATGTCATTGTAGCAAGATTTGATGGCAGAGGAAGTGGATTCCA
  GGGCCTCAAAGTTTTGCAGGAGATTCACAGAAGGACAGGCTCAGTAGAGGCAAAGGACCAAATAGCAGCT
  ATAAAATATTTACTGAAACAGCCATATATTGACTCCAAAAGATTAAGCATTTTTGGAAAGGGATATGGGG
  GATACATTGCATCAATGATCCTAAAATCAGATGAGAAGTTTTTCAAATGTGGAACCGTGGTTGCACCCAT
  CTCAGACATGAAGTTGTATGCCTCAGCTTTCTCTGAGAGGTACCTTGGTATGCCATCAAAGGAAGAAAGC
  ACTTACCAGGCATCCAGTGTACTGCATAACATTCATGGTTTAAAAGAAGAAAATTTATTAATAATTCACG
  GAACTGCTGATACAAAAGTTCATTTCCAGCATTCAGCAGAATTGATCAAGCATCTGATAAAAGCTGGGGT
  GAATTACACTCTGCAGGTCTATCCAGATGAAGGATATCACATTTCAGACAAGAGCAAGCATCATTTTTAC
  AGCACAATTCTCAGATTCTTCAGTGATTGCCTAAAGGAAGAAGTATCCGTGCTGCCACAGGAACCAGAAG
  AAGATGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR212272 representing NM_001012205
  Red=Cloning site Green=Tags(s)
  
  MNQTASVSHHIKCQPSKTIKELGSNSPPQRNWKGIAIALLVILVVCSLITMSVILLTPDELTNSSETRLS
  LEELLGKGFGLHNPEARWINDTDVVYKTDNGHVMKLNAETNATTLLLDNSTFVTFKASRHSLSPDLKYVL
  LAYDVKQIFHYSFTASYLIYNIHTGEVWELNPPEVEDSVLQYAAWGVQGQQLIYIFENNIYYQPDIKSSS
  LRLTSSGKEGIVFNGIADWLYEEELLHSHIAHWWSPDGERLAFLMINDSLVPNMVIPRFTGALYPKAKQY
  PYPKAGQANPSVKLYVVNLYGPTHTLELMPPDIFKSREYYITMVKWVSNTRTVVRWLNRPQNISILTVCE
  STTGACSRKYEMTSDTWISKQNEEPVFSRDGSKFFMTVPVKQGGRGEFHHIAMFLVQSKSEQITVRHLTS
  GNWEVIRILAYDETTQKIYFLSTEFSPRGRQLYSASTEGLLSRDCISCNFRKEDCTYFDASFSPMNQHFL
  LFCEGPKVPMVSLHSTDNPSNYYILERNSMMKETIQKKKLAKREIRILHIDDYELPLQLSFPKDFLEKNQ
  YALLLIIDEEPGGQMVTEKFHVDWDSVLIDTDNVIVARFDGRGSGFQGLKVLQEIHRRTGSVEAKDQIAA
  IKYLLKQPYIDSKRLSIFGKGYGGYIASMILKSDEKFFKCGTVVAPISDMKLYASAFSERYLGMPSKEES
  TYQASSVLHNIHGLKEENLLIIHGTADTKVHFQHSAELIKHLIKAGVNYTLQVYPDEGYHISDKSKHHFY
  STILRFFSDCLKEEVSVLPQEPEEDE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001012205
• ORF Size: 2388 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012205.1, NP_001012205.1
• RefSeq Size: 2391 bp
• RefSeq ORF: 2391 bp
• Locus ID: 363972
• Cytogenetics: 13q11-q12
• MW: 90.9 kDa