Soat1 (NM_031118) Rat Tagged ORF Clone

CAT#: RR212950

Soat1 (Myc-DDK-tagged ORF) - Rat sterol O-acyltransferase 1 (Soat1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Soat1
• Synonyms: Acat-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR212950 representing NM_031118
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGGAGAAGAAACGTCTCTGAGAAACCGGCTGTCAAGATCTGCTGAGAATCCTGAGCAAGATGAAG
  CCCAGAAAAATCTCTTGGACACACACAGAAATGGTCACATTACCATGAAACAGTTGATAGCCAAGAAGAG
  GCAGTTGGCGGCAGAGGCAGAGGAGCTGAAGCCACTGTTTCTGAAGGAAGTTGGGTGTCACTTTGATGAC
  TTTGTGACCAATCTCATTGACAAGTCTGCATCTCTGGACAATGGCGGGTGTGCGCTCACGACCTTCTCCA
  TTCTTGAAGAAATGAAAAACAACCACAGAGCCAAAGATCTGAGAGCACCTCCAGAACAAGGGAAGATATT
  TATTTCAAGGCGGTCTCTTTTAGACGAGCTGTTTGAAGTGGACCACATCAGAACAATTTACCACATGTTC
  ATTGCCCTCCTCATCATCTTCATCCTTAGCACGCTCGTGGTGGATTACATCGATGAAGGAAGGCTGGTCC
  TTGAGTTCAGTCTCCTGGCTTATGCTTTTGGCCAATTTCCTATTGTTATTTGGACATGGTGGGCCATGTT
  CCTGTCTACACTGGCAATCCCCTATTTCCTGTTCCAGCGTTGGGCCCATGGTTACAGCAAGAGTTCCCAC
  CCACTCATCTATTCCCTTATCCATGGCGCATTCTTCTTGGTCTTTCAACTTGGAATCCTCGGTTTTATAC
  CAACATATGTTGTCTTAGCATACACGCTGCCCCCAGCCTCCCGGTTCATTCTGATACTTGAGCAGATTCG
  TTTGGTAATGAAGGCTCACTCATATGTCAGAGAGAATGTGCCACGAGTTCTAAGTGCAGCCAAGGAGAAA
  TCGAGCACGGTTCCAGTACCCACAGTCAACCAGTACCTGTACTTCCTGTTTGCTCCTACACTTATTTACC
  GAGACAGCTACCCCAGGACTCCTACTGTAAGATGGGGTTATGTTGCTATGCAGTTTTTACAGGTTTTTGG
  TTGCCTGTTTTATGTGTACTACATCTTTGAGAGACTCTGTGCCCCTCTGTTCCGGAATATCAAACAGGAG
  CCCTTTAGTGCTCGTGTCCTGGTCCTGTGTGTATTTAACTCCATCTTGCCAGGTGTGCTGATGCTGTTCC
  TTTCGTTCTTTGCCTTTTTGCACTGCTGGCTCAATGCCTTTGCTGAAATGTTACGCTTTGGTGACAGGAT
  GTTTTATAAGGACTGGTGGAACTCTACATCGTACTCCAACTACTACAGGACCTGGAATGTGGTGGTCCAC
  GACTGGCTCTACTACTATGTTTACAAGGACCTCCTCTGGTTTTTCTCGAAGAGGTTCAGGCCTGCCGCCA
  TGTTGGCCGTGTTCGCCCTGTCAGCAGTGGTGCACGAGTACGCCCTCGCTGTCTGCCTGAGCTACTTCTA
  CCCAGTGCTCTTCGTGCTCTTCATGTTCTTTGGAATGGCTTTTAACTTCATCGTTAACGACAGTCGGAAA
  AGGCCAGTCTGGAACATCATGGTTCGGGCTTCCCTCTTCCTCGGCCATGGAGTCATTCTGTGCTTTTACT
  CTCAAGAGTGGTATGCTCGACAGCGCTGTCCTCTGAAGAACCCTACATTTCTGGATTATGTCCGGCCACG
  GACCTGGACTTGTCGTTACGTGTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR212950 representing NM_031118
  Red=Cloning site Green=Tags(s)
  
  MVGEETSLRNRLSRSAENPEQDEAQKNLLDTHRNGHITMKQLIAKKRQLAAEAEELKPLFLKEVGCHFDD
  FVTNLIDKSASLDNGGCALTTFSILEEMKNNHRAKDLRAPPEQGKIFISRRSLLDELFEVDHIRTIYHMF
  IALLIIFILSTLVVDYIDEGRLVLEFSLLAYAFGQFPIVIWTWWAMFLSTLAIPYFLFQRWAHGYSKSSH
  PLIYSLIHGAFFLVFQLGILGFIPTYVVLAYTLPPASRFILILEQIRLVMKAHSYVRENVPRVLSAAKEK
  SSTVPVPTVNQYLYFLFAPTLIYRDSYPRTPTVRWGYVAMQFLQVFGCLFYVYYIFERLCAPLFRNIKQE
  PFSARVLVLCVFNSILPGVLMLFLSFFAFLHCWLNAFAEMLRFGDRMFYKDWWNSTSYSNYYRTWNVVVH
  DWLYYYVYKDLLWFFSKRFRPAAMLAVFALSAVVHEYALAVCLSYFYPVLFVLFMFFGMAFNFIVNDSRK
  RPVWNIMVRASLFLGHGVILCFYSQEWYARQRCPLKNPTFLDYVRPRTWTCRYVF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031118
• ORF Size: 1635 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_031118.1, NP_112380.1
• RefSeq Size: 1750
• RefSeq ORF: 1638
• Locus ID: 81782
• MW: 64.1 kDa
• Gene Summary: catalyzes the conversion of free cholesterol and fatty acyl-CoA to cholesteryl esters; plays a role in cholesterol metabolism [RGD, Feb 2006]