Armc5 (NM_001009455) Rat Tagged ORF Clone

CAT#: RR213148

Armc5 (Myc-DDK-tagged ORF) - Rat armadillo repeat containing 5 (Armc5), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Armc5
• Synonyms: MGC94568
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR213148 representing NM_001009455
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCTGCGAGACCGGCCCTTACGGACTCGCTCTCGTTTTGCCTCGCGCAACTCACGGCAGCGGCCG
  GGGAGGGGCCAGGTGGGGGAAAGGACCCAGCTACCAACGAGACAGCCCTGGGCCGCGTGCTCTTAGCTCT
  TCGCACGCGCCACATCAAGGCAGCAGAGGGAATTGAGCGCTTCCGGGCACGCGGCGGGCTCCGCCCCCTT
  GTCGCCCTGCTACGCCGAACGGCTGCTGCGGGCCCCGCCCCGTCCCAAGCAGCCTCTGGCTCCGCCCCTC
  CGTCGGTTGCGTCAGCTGGTTCTTCCCCTGGCCCCGCCCCCGCTGCTGAGTCGCCTTTGACTCTTTCGGC
  GCCAATGCGCCTGCGCAAGACGCTGGATTTGGCGCTCAGCATTCTAGCCAACTGCTGTACAGAAGGGGCG
  TGCCGGTCTGAAGTGCGTAGAATTGGAGGCATTCTCCCTTTGGTGACCATTCTTCAGTGTGTGAGGATAG
  ACAGCATCCAGAACCGAACAGCCCGTGCTCTGGGGAACTTAGCCATGGAGCCTGAGAGCTGCGGGGACAT
  CCACTCTGCTGGGGCTGTTCCCTTTCTTGTTGAGAGCCTTACTGCCTGCCAGGACTCACAGTGCCTACAG
  AGCATAGTTCGTGCCCTCCGCAACTTGGCAGACTCACCCCAGCACCGCCTAGCCCTGGCACAGCAAGGAG
  CAGTGCGGCCACTGGCCGAGCTCCTGGCTACTGCCCCAGACCCTGCACTGACCTCAGCCCTAGTCCGTGC
  TCTCTTGGAACTCAGCCGAGGCTGTTCCCGAGCCTGTGCTGAGCAGCTAAGTCTGGGTGGGGGACTGGGC
  CCACTTGTCAGCCTGGCTTCTCACCCTAAGCGGGCAATACGTGAGGCAGCCATTCTGATCCTTGCCAATC
  TGTGTGCTCAGGGCCTGGTGCGACCTGCACTGGGCAATGCTGGTGGTGTGGAGGTGTTACTAGGTGAGCT
  TAGGCGGCGACGGGGTCCCAGTGGGAGCAGCTCAGCTTCCCAGCAGCCCCTAGTGCGGGCTGTGTGCCTC
  TTATGCCGGGAAGCCATCAACCGGGCTCGGCTTCGGGATGCTGGTGGTTTGGAGCTGCTGATGGGTCTAT
  TGCAGGACCCTGGTGCCAGTGCCTGGCATCCACGTGTTGTGGCTGCTCTTGTAGGCTTCCTTTATGATAC
  TGGGGCCTTGGGCAAGTTACAGGCTCTGGGACTTGTACCTCTTCTGGCCAGGCAGCTATGTGGTGAAGCT
  GGCGAAGAGGAAGAAGAAGGAGTAGAAGCTGCTTCCTGGGACTTCCCCGAGGAACGGACCTCTGGGCAGC
  CGGAGGGAGGAAGCTTCCGAAGCCTCAGGTTGTGGCTGATTTCTGAGGGTTATGCAGCAGGCCCTGGAGA
  TATCTCCCCAGATTGGTCTCCTGAGCGTTGTCCGATGCCAGAGCCATCTGAGTCAGTCAGTCCTACCCCT
  GGCCAGACCTCAATGTCGACACCCCGCACACTTCGCAAACTGGGCCGTGTCCCTGCTGCCACTACTGAGG
  AGCCTTGGGGCCAAGAGGGGCCAGCACTGTTGCTGTTGTCACGCTTCTCCCAGGCTCCTGACCCAAGTGG
  AGCTCTAGTGACTGGTCCAGCACTGTGTGGCCTGTTGGCCTATGTGACAGGTGCACCGGGACCACCAAAC
  CCACGTGCACTGCGTATCTTGGCACGCCTCACCTGTAACCCTGCGTGCCTTGAGGCCTTTGTGCGCAGTT
  ATGGTGCAGCACTGCTGCGTGCCTGGCTGGTCCTCGGTGTCTCACCAGATGACTGGCCTGTGCCACATGC
  CCGGCCTGTACACCGAAGCCAGCACCGAGAGCTGGGTGAGATGCTGCTGCAGAACCTGACTGTCCAAGCT
  GAGTCCCCCTTCGGAGTGGGCGCCCTCACCCACCTGCTGCTCTCTGGGAGCCCTGAGGACCGTGTAGCCT
  GTGCACTCACCGTACCCTTCATCTGCCGGAAGCCCACTCTGTGGCGCCGCTTACTTCTGGATCAGGGTGG
  CCTCCGCCTCCTCCTCACAGCACTAACGCAGCCAGCTCCACACCCACTCTTCCTCTTCTTTGCAGCAGAT
  TCCCTGTCCTGTCTCCAAGGCCTGGTGTCTCCCTCTGCAAGCCCAGTCCCTTTACCTGACCTGCCCTTGG
  AACTGGACTCACCCTCCCCTTGCCTCTACAAACCTCTGCTGGGTCCAGCCCCTGCCCCAGCTCCTGACCT
  GCACTTCGTTCTGGACTCAGGCCTACAGCTCCCTGCTCAGCGGGCTGCTTCAGCTGCTGCCTCCCCTTTC
  TTCCGGGCCCTGCTATCCGGCAGCTTTGCTGAAGCCCAGATGAATTTGGTGCCACTTCGAGGCCTGTCCC
  CTAGTGCAGCGTGGCCTGTCCTACACCATTTGCATGGCTGCCGGGGCTGTGGGGCTGCTCTGGGACCTAT
  ACCCCCACCTGGTCAGCCCCTGCTGGGCTCTAAGGCTGAGGAAGCTCTAGAAGCTGCTGGCCGATTCTTG
  CTGCCTGCACTAGAGGAAGAGCTAGAAGAAGCTGTTGGCCGCATCCACTTGAGTCCACAGGGTGGCCCAG
  AGTCAGTGGGTGAGGTATTCCGTCTAGGCCGGCCCCGGCTGGCTGCCCACTGTGCCAGATGGACACTAGA
  ACCAGGGCAGTGCCCAAGAAAACGGGCCCTGGCCCTGACGGGGCTGGTGGAAGCAGCAGGTGAGGAGGCA
  GGGCCCCTCACTGAGGCTTTGCTGGCTGTGGTAATGGGGGTTGAGTCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR213148 representing NM_001009455
  Red=Cloning site Green=Tags(s)
  
  MAAARPALTDSLSFCLAQLTAAAGEGPGGGKDPATNETALGRVLLALRTRHIKAAEGIERFRARGGLRPL
  VALLRRTAAAGPAPSQAASGSAPPSVASAGSSPGPAPAAESPLTLSAPMRLRKTLDLALSILANCCTEGA
  CRSEVRRIGGILPLVTILQCVRIDSIQNRTARALGNLAMEPESCGDIHSAGAVPFLVESLTACQDSQCLQ
  SIVRALRNLADSPQHRLALAQQGAVRPLAELLATAPDPALTSALVRALLELSRGCSRACAEQLSLGGGLG
  PLVSLASHPKRAIREAAILILANLCAQGLVRPALGNAGGVEVLLGELRRRRGPSGSSSASQQPLVRAVCL
  LCREAINRARLRDAGGLELLMGLLQDPGASAWHPRVVAALVGFLYDTGALGKLQALGLVPLLARQLCGEA
  GEEEEEGVEAASWDFPEERTSGQPEGGSFRSLRLWLISEGYAAGPGDISPDWSPERCPMPEPSESVSPTP
  GQTSMSTPRTLRKLGRVPAATTEEPWGQEGPALLLLSRFSQAPDPSGALVTGPALCGLLAYVTGAPGPPN
  PRALRILARLTCNPACLEAFVRSYGAALLRAWLVLGVSPDDWPVPHARPVHRSQHRELGEMLLQNLTVQA
  ESPFGVGALTHLLLSGSPEDRVACALTVPFICRKPTLWRRLLLDQGGLRLLLTALTQPAPHPLFLFFAAD
  SLSCLQGLVSPSASPVPLPDLPLELDSPSPCLYKPLLGPAPAPAPDLHFVLDSGLQLPAQRAASAAASPF
  FRALLSGSFAEAQMNLVPLRGLSPSAAWPVLHHLHGCRGCGAALGPIPPPGQPLLGSKAEEALEAAGRFL
  LPALEEELEEAVGRIHLSPQGGPESVGEVFRLGRPRLAAHCARWTLEPGQCPRKRALALTGLVEAAGEEA
  GPLTEALLAVVMGVES
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001009455
• ORF Size: 2778 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001009455.1, NP_001009455.1
• RefSeq Size: 3289 bp
• RefSeq ORF: 2781 bp
• Locus ID: 361653
• Cytogenetics: 1q37
• MW: 96.6 kDa