Cyld (NM_001017380) Rat Tagged ORF Clone

CAT#: RR213203

Cyld (Myc-DDK-tagged ORF) - Rat cylindromatosis (turban tumor syndrome) (Cyld), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cyld
• Synonyms: LRRGT00003; Rp1; Rp1h
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR213203 representing NM_001017380
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTTCAGGCCTGTGGAACCAAGAGAAAGTTACTTCACCCTACTGGGAAGAACGGCTTTTTTATCTGC
  TTCTTCAAGAATGCAGTGTAACAGACAAACAGACACAGAAGCTCCTGAGAGTACCCAAAGGGAGCATAGG
  ACAGTACATCCAAGACCGTTCCGTGGGGCATTCAAGAGTTCCTTCTGCTAAAGGCAAGAAAAATCAGATT
  GGATTAAAAATCTTAGAGCAACCGCATGCAGTTCTGTTTGTTGATGAAAAGGATGTTGTAGAAATAAATG
  AAAAATTCACAGAGTTACTGTTGGCAATTACCAACTGTGAGGAGAGGCTCAGCCTATTTAGAAACAGAAT
  CCGACTAAGTAAAGGCCTCCAGGTAGACGTGGGCAGTCCTGTGAGAGTACAGCTGCGATCTGGGGAGGAG
  AAGTTTCCAGGAGTTGTACGCTTCAGAGGACCTTTATTAGCGGAGAGGACGGTGTCGGGGATTTTCTTTG
  GAGTAGAATTACTGGAAGAAGGTCGTGGCCAAGGTTTCACTGATGGGGTGTATCAAGGAAAACAGCTCTT
  CCAGTGTGATGAGGACTGTGGCGTTTTTGTTGCATTGGACAAGCTGGAGCTTATAGAAGATGATGACAAT
  GGGTTGGAAAGTGATTTTGCAGGCCCAGGAGATACAGTCCAGGTTGAACCTCCCCCTTTGGAAATAAACT
  CCAGAGTTTCTTTGAAGGTTGGAGAAAGTACAGAATCTGGAACAGTGATATTCTGTGATGTTTTACCAGG
  AAAAGAGAGTCTAGGATATTTTGTTGGTGTGGACATGGATAACCCTATTGGCAACTGGGACGGAAGGTTT
  GATGGGGTTCAGCTTTGCAGTTTTGCAAGTGTTGAGAGTACAGTTCTCCTACACATCAATGACATCATCC
  CAGATAGCGTGACACAGGAAAGGAGACCTCCCAAACTTGCCTTTATGTCAAGAGGTGTAGGTGACAAAGG
  TTCATCTAGTCATAATAAACCAAAGGTTACAGGATCTACCTCAGACCCTGGAAGTAGAAACAGATCTGAA
  TTATTTTATACCTTAAATGGGTCATCTGTTGACTCACAACAACAATCCAAGTCCAAAAACCCATGGTACA
  TTGATGAAGTTGCAGAAGACCCTGCAAAGTCACTTACAGAGATGTCTTCAGACTTCGGACATTCATCGCC
  TCCACCGCAACCTCCTTCCATGAACTCCTTGTCTAGCGAGAACAGATTCCACTCCTTACCCTTCAGCCTG
  ACAAAGATGCCCAACACTAATGGCAGCATGGCTCACAGTCCACTCTCTCTGTCAGTGCAGTCTGTGATGG
  GAGAGCTGAACAGCACTCCTGTCCAGGAGAGTCCACCCATGCCCAGCTCTTCTGGGAATGCACACGGGCT
  AGAGGTGGGCTCACTGGCTGAAGTAAAAGAGAACCCCCCGTTCTATGGGGTTATCCGTTGGATTGGCCAG
  CCACCAGGGCTCAGTGACGTGCTTGCTGGATTGGAACTGGAAGATGAATGCGCAGGTTGTACGGATGGAA
  CTTTCAGGGGCACGCGCTATTTCACCTGTGCCCTGAAGAAAGCACTGTTCGTGAAACTGAAGAGCTGCAG
  ACCAGACTCTAGGTTTGCATCCTTGCAGCCTGTTTCCAATCAGATCGAAAGGTGTAACTCTTTAGCATTT
  GGGGGCTACTTAAGTGAAGTAGTAGAAGAAAATACGCCACCTAAAATGGAAAAGGAAGGTTTAGAGATAA
  TGATTGGAAAGAAGAAAGGCATCCAGGGCCATTACAATTCTTGTTACTTAGACTCAACTTTATTCTGCTT
  ATTTGCTTTTAGTTCTGCCCTGGACACTGTATTACTTAGACCCAAAGAGAAGAATGATGTAGAGTATTAC
  AGTGAGACTCAAGAGCTACTGAGGACAGAGATAGTCAATCCTCTGAGAATATATGGATATGTGTGTGCCA
  CAAAGATTATGAAGCTGAGGAAAATACTTGAAAAAGTTGAGGCTGCATCAGGATTTACCTCTGAGGAAAA
  AGATCCTGAAGAATTTCTAAACATCCTGTTTCATGATATTTTAAGGGTTGAACCATTGTTAAAAATAAGG
  TCAGCAGGTCAAAAAGTTCAAGACTGTAACTTCTATCAAATTTTTATGGAAAAAAATGAGAAAGTCGGAG
  TACCCACAATCCAGCAGTTATTAGAATGGTCTTTTATCAACAGCAACCTGAAATTTGCAGAGGCACCATC
  ATGCTTGATTATCCAGATGCCTCGGTTTGGGAAAGACTTTAAACTATTTAAAAAAATTTTTCCTTCCCTG
  GAATTAAATATAACAGATTTACTTGAAGACACTCCCAGGCAGTGCCGCATCTGTGGAGGACTCGCCATGT
  ATGAGTGTAGAGAGTGCTATGATGACCCGGACATCTCGGCAGGGAAGATCAAGCAGTTCTGTAAGACCTG
  CAGCACTCAGGTTCACCTTCATCCCAGAAGACTGAATCACACTTACCATCCAGTATCACTTCCCAAAGAC
  TTGCCCGACTGGGACTGGAGACACGGCTGCATCCCGTGTCAGAAGATGGAGTTATTTGCTGTGCTCTGCA
  TAGAAACCAGCCACTATGTTGCTTTTGTGAAGTACGGGAAGGATGACTCTGCCTGGCTCTTCTTTGACAG
  CATGGCTGATCGAGATGGTGGTCAGAATGGCTTCAACATTCCACAAGTGACACCCTGCCCAGAAGTAGGA
  GAGTACTTGAAGATGTCTCTGGAGGACCTGCACTCTTTGGACTCCAGAAGGATTCAAGGCTGTGCGCGCA
  GACTTCTTTGCGATGCATACATGTGCATGTACCAGAGTCCAACCATGAGCTTGTACAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR213203 representing NM_001017380
  Red=Cloning site Green=Tags(s)
  
  MSSGLWNQEKVTSPYWEERLFYLLLQECSVTDKQTQKLLRVPKGSIGQYIQDRSVGHSRVPSAKGKKNQI
  GLKILEQPHAVLFVDEKDVVEINEKFTELLLAITNCEERLSLFRNRIRLSKGLQVDVGSPVRVQLRSGEE
  KFPGVVRFRGPLLAERTVSGIFFGVELLEEGRGQGFTDGVYQGKQLFQCDEDCGVFVALDKLELIEDDDN
  GLESDFAGPGDTVQVEPPPLEINSRVSLKVGESTESGTVIFCDVLPGKESLGYFVGVDMDNPIGNWDGRF
  DGVQLCSFASVESTVLLHINDIIPDSVTQERRPPKLAFMSRGVGDKGSSSHNKPKVTGSTSDPGSRNRSE
  LFYTLNGSSVDSQQQSKSKNPWYIDEVAEDPAKSLTEMSSDFGHSSPPPQPPSMNSLSSENRFHSLPFSL
  TKMPNTNGSMAHSPLSLSVQSVMGELNSTPVQESPPMPSSSGNAHGLEVGSLAEVKENPPFYGVIRWIGQ
  PPGLSDVLAGLELEDECAGCTDGTFRGTRYFTCALKKALFVKLKSCRPDSRFASLQPVSNQIERCNSLAF
  GGYLSEVVEENTPPKMEKEGLEIMIGKKKGIQGHYNSCYLDSTLFCLFAFSSALDTVLLRPKEKNDVEYY
  SETQELLRTEIVNPLRIYGYVCATKIMKLRKILEKVEAASGFTSEEKDPEEFLNILFHDILRVEPLLKIR
  SAGQKVQDCNFYQIFMEKNEKVGVPTIQQLLEWSFINSNLKFAEAPSCLIIQMPRFGKDFKLFKKIFPSL
  ELNITDLLEDTPRQCRICGGLAMYECRECYDDPDISAGKIKQFCKTCSTQVHLHPRRLNHTYHPVSLPKD
  LPDWDWRHGCIPCQKMELFAVLCIETSHYVAFVKYGKDDSAWLFFDSMADRDGGQNGFNIPQVTPCPEVG
  EYLKMSLEDLHSLDSRRIQGCARRLLCDAYMCMYQSPTMSLYK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001017380
• ORF Size: 2859 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001017380.1, NP_001017380.1
• RefSeq Size: 3267 bp
• RefSeq ORF: 2862 bp
• Locus ID: 312937
• Cytogenetics: 19p11
• MW: 106.7 kDa