Hcn2 (NM_053684) Rat Tagged ORF Clone

CAT#: RR213404

Hcn2 (Myc-DDK-tagged ORF) - Rat hyperpolarization activated cyclic nucleotide-gated potassium channel 2 (Hcn2), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hcn2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR213404 representing NM_053684
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGCGCGCGGGGGCGGCGGGCGGCCGGGCGATAGTCCGGGCGCGACCCCTGCGCCGGGGCCGCCGC
  CACCGCCGCCGCCGCCCGCGCCCCCTCAGCCTCAGCCACCACCCGCGCCACCCCCGAACCCCACGACCCC
  CTCGCACCCGGAGTCGGCGGACGAGCCCGGCCCGCGCTCCCGGCTCTGCAGCCGCGACAGCTCCTGCACT
  CCTGGCGCGGCCAAGGGCGGCGCGAATGGCGAGTGCGGGCGCGGGGAGCCGCAGTGCAGCCCCGAGGGCC
  CCGCGCGCGGCCCCAAGGTTTCGTTCTCATGTCGCGGGGCGGCCTCGGGGCCCGCGGCGGCCGAGGAGGC
  GGGCAGCGAGGAGGCGGGCCCGGCGGGTGAGCCGCGCGGCAGCCAGGCCAGCTTCCTGCAGCGCCAATTC
  GGGGCGCTCCTGCAGCCGGGCGTCAACAAGTTCTCCCTGCGGATGTTTGGCAGCCAGAAGGCCGTGGAGC
  GCGAGCAGGAACGCGTGAAGTCGGCGGGGGCCTGGATCATCCACCCCTACAGCGACTTCAGGTTCTACTG
  GGACTTCACCATGCTGTTGTTCATGGTGGGAAATCTCATCATCATCCCTGTGGGCATCACTTTCTTCAAG
  GACGAGACCACGGCGCCCTGGATCGTCTTCAACGTGGTCTCGGACACTTTCTTCCTCATGGACTTGGTGC
  TGAACTTTCGCACCGGCATTGTTATTGAGGACAACACGGAGATCATCCTGGACCCCGAAAAGATAAAGAA
  AAAGTACCTGCGTACGTGGTTCGTGGTAGACTTCGTGTCATCCATCCCGGTGGACTACATCTTCCTCATC
  GTGGAGAAGGGAATCGACTCCGAGGTCTACAAGACGGCCCGTGCACTACGCATCGTGCGTTTCACCAAGA
  TCCTCAGTCTGCTGCGGTTGCTGCGGCTATCCCGGCTCATCCGATATATCCACCAATGGGAGGAGATTTT
  CCACATGACCTACGACCTGGCAAGCGCGGTGATGCGCATCTGTAACCTGATCAGCATGATGCTGCTGCTC
  TGCCACTGGGACGGCTGCCTGCAGTTCCTGGTGCCCATGCTGCAAGACTTCCCCAGCGACTGCTGGGTGT
  CCATCAACAACATGGTGAACCACTCGTGGAGCGAACTCTATTCGTTCGCGCTCTTCAAGGCCATGAGCCA
  CATGCTCTGTATTGGCTACGGGCGGCAGGCTCCCGAGAGCATGACGGACATCTGGCTCACCATGCTCAGC
  ATGATCGTGGGCGCCACCTGCTACGCTATGTTCATTGGGCACGCCACGGCGCTTATCCAGTCCCTGGACT
  CGTCACGGCGCCAGTACCAGGAGAAGTACAAGCAAGTGGAGCAGTACATGTCCTTCCACAAACTGCCGGC
  TGACTTCCGCCAGAAGATCCACGATTACTATGAACACCGGTACCAGGGGAAGATGTTTGACGAGGACAGC
  ATCCTGGGGGAACTCAACGGCCCACTGCGTGAGGAGATTGTGAACTTCAACTGCCGGAAGCTGGTGGCTT
  CCATGCCGTTGTTTGCCAACGCAGACCCCAACTTCGTCACCGCCATGCTGACAAAGCTCAAATTTGAGGT
  CTTCCAGCCTGGAGACTACATCATCCGAGAGGGGACCATCGGGAAGAAGATGTACTTCATCCAGCACGGG
  GTGGTGAGCGTGCTCACCAAGGGCAACAAGGAGATGAAGCTGTCAGATGGCTCCTATTTTGGGGAGATCT
  GCCTGCTCACGAGGGGCCGGCGCACAGCCAGTGTGCGGGCTGACACCTACTGTCGCCTCTACTCACTGAG
  CGTGGACAACTTCAACGAGGTGCTGGAGGAGTACCCCATGATGCGGCGTGCCTTTGAGACCGTGGCCATT
  GACCGCCTGGACCGCATAGGCAAGAAGAACTCCATCTTGCTACACAAGGTTCAGCATGATCTCAGCTCGG
  GTGTGTTCAACAACCAGGAGAACGCCATCATCCAGGAGATTGTCAAATATGACCGTGAGATGGTGCAGCA
  GGCAGAGCTGGGCCAGCGTGTGGGGCTCTTCCCACCACCGCCACCACCGCAGGTCACGTCGGCCATCGCC
  ACACTGCAGCAGGCCGTGGCCATGAGCTTCTGCCCGCAGGTGGCCCGCCCGCTCGTGGGGCCCCTGGCGC
  TAGGGTCCCCACGCCTCGTGCGCCGCGCGCCCCCAGGGCCTCTGCCTCCTGCAGCCTCACCAGGGCCACC
  CGCAGCGAGCCCCCCGGCTGCACCCTCGAGCCCTCGGGCACCGCGGACCTCACCCTACGGTGTGCCTGGC
  TCTCCGGCAACGCGTGTGGGGCCCGCATTGCCCGCACGCCGCCTGAGCCGCGCCTCGCGCCCACTGTCCG
  CCTCGCAGCCCTCGCTGCCCCACGGCGCGCCCGCACCCAGCCCCGCGGCCTCTGCGCGCCCGGCCAGCAG
  CTCCACCCCGCGTCTGGGACCCGCACCCACCACCCGGACCGCGGCACCCAGTCCGGACCGCAGGGACTCA
  GCCTCGCCGGGCGCTGCCAGTGGCCTCGACCCACTGGACTCTGCGCGCTCGCGCCTCTCTTCCAACTTG
  
  ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA
  TTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR213404 representing NM_053684
  Red=Cloning site Green=Tags(s)
  
  MDARGGGGRPGDSPGATPAPGPPPPPPPPAPPQPQPPPAPPPNPTTPSHPESADEPGPRSRLCSRDSSCT
  PGAAKGGANGECGRGEPQCSPEGPARGPKVSFSCRGAASGPAAAEEAGSEEAGPAGEPRGSQASFLQRQF
  GALLQPGVNKFSLRMFGSQKAVEREQERVKSAGAWIIHPYSDFRFYWDFTMLLFMVGNLIIIPVGITFFK
  DETTAPWIVFNVVSDTFFLMDLVLNFRTGIVIEDNTEIILDPEKIKKKYLRTWFVVDFVSSIPVDYIFLI
  VEKGIDSEVYKTARALRIVRFTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLASAVMRICNLISMMLLL
  CHWDGCLQFLVPMLQDFPSDCWVSINNMVNHSWSELYSFALFKAMSHMLCIGYGRQAPESMTDIWLTMLS
  MIVGATCYAMFIGHATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPADFRQKIHDYYEHRYQGKMFDEDS
  ILGELNGPLREEIVNFNCRKLVASMPLFANADPNFVTAMLTKLKFEVFQPGDYIIREGTIGKKMYFIQHG
  VVSVLTKGNKEMKLSDGSYFGEICLLTRGRRTASVRADTYCRLYSLSVDNFNEVLEEYPMMRRAFETVAI
  DRLDRIGKKNSILLHKVQHDLSSGVFNNQENAIIQEIVKYDREMVQQAELGQRVGLFPPPPPPQVTSAIA
  TLQQAVAMSFCPQVARPLVGPLALGSPRLVRRAPPGPLPPAASPGPPAASPPAAPSSPRAPRTSPYGVPG
  SPATRVGPALPARRLSRASRPLSASQPSLPHGAPAPSPAASARPASSSTPRLGPAPTTRTAAPSPDRRDS
  ASPGAASGLDPLDSARSRLSSNL
  
  TRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-NotI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053684
• ORF Size: 2589 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_053684.1, NP_446136.1
• RefSeq Size: 2633
• RefSeq ORF: 2592
• Locus ID: 114244
• MW: 94.7 kDa
• Gene Summary: may play a role in generation of neuronal pacemaker activity [RGD, Feb 2006]