Hars2 (NM_001014012) Rat Tagged ORF Clone

CAT#: RR213422

Hars2 (Myc-DDK-tagged ORF) - Rat histidyl-tRNA synthetase 2-like (Hars2l), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hars2
• Synonyms: Hars2l; RGD1308426; Zmat2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR213422 representing NM_001014012
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCCACCTAGGGCCCCTGCGCTGTAGGACCTGGGCTGCACTACTCGGCCAACTCCTGCGACCTCCCC
  GCACTGTGTGCAACCGGGCGGTCGGCTGTCATAGCCAGGTTGCGAAGGCAGTGTTAACATCTGAGCAACT
  GAAACCACATCAACAAAAACCAAATTTTATCATCAAGGTTCCAAAGGGCACCAGGGATTTAAGTCCTCAA
  CAGATGGTTGTGAGGGAGAAAATTCTTGATAAGATTATTAGCTGTTTTAAACGTCATGGGGCAAAGGGGT
  TGGACACGCCAGCATTTGAGCTAAAAGAAATGCTCACCGAGAAGTATGAAGACAACTTCGGCCTCATGTA
  TGATTTGGAGGATCAAGGTGGGGAGTTGCTGTCTCTGCGCTATGACCTTACTGTCCCTTTTGCTCGCTAT
  CTGGCCATGAATAAATTGAAGAGGATGAAACGGTATCAAGTGGGAAAGGTGTGGAGGCGAGAGAGTCCAG
  CTATAGTCCAGGGCCGCTACCGGGAGTTCTGCCAGTGTGATTTTGACATTGCTGGTGAGTTTGACCCAAT
  GATCCCTGATGCAGAGTGTTTGAGGATTATGTGTGAAATCCTAAGTGGATTGCAGCTGGGGGACTTTCTC
  ATTAAGGTAAATGACCGTCGGGTTGTAGACGGGATGTTTGCTGTCTGTGGTGTTCCTGAGAGCAAGTTCC
  GTACCATCTGCTCCTCAATGGACAAACTAGACAAGATGTCTTGGGAAGATGTGAGACTTGAGATGGTGGC
  AGGGAAAGGCCTAGCTCCTGAGGTGGCTGATCGAATTGGGGACTACGTCCAATGCCATGGGGGGATATCC
  CTGGTAGAGGAGCTGTTCAAGGATCCCAGACTGTCCCAAAGCCAGCTGGCCTTGCAGGGCCTAGGGGACC
  TGAAGCTGCTCTTTGAATACCTGAGGTTATTTGGAATTGCCGATAAGATCTCCCTTGATCTGAGTCTGGC
  CCGGGGCCTGGACTATTATACAGGAGTCATTTATGAAGCAGTACTGCTAGAGTCTCCAGCTCAGGCCGGA
  AAGGAGACTCTGAATGTGGGTAGTGTGGCTGCTGGTGGGCGCTATGACAGGCTGGTAGCCCAGTTTGATC
  CCAAGGGACATAGCGTGCCTTGTGTGGGGTTGAGCATTGGAGTAGAGAGAATCTTCTATCTTGTGGAGCA
  GAAGATGAAGATTTCTTGTGAGAAGGTTCGAACCACAGAGACCCAAGTGTTTGTGGCCACGCCCCAGAAG
  AACTTCCTCCAAGAGCGTTTGAAGATAATTGCACAGCTTTGGGATGCAGGGATCAAGGCAGAGATGCTTT
  ATAAAAACAACCCTAAACTGTTAACCCAGCTGCACTATTGTGAGAAAGAAGACATTCCTCTAATGGTCAT
  TATTGGTGAGCAAGAACAGAATGAAGGTGTCGTCAAGCTCCGTTCAGTGGCCAGCAGAGAAGAAGTGACC
  GTTAATCGAGAAAGTCTTGTGGCTGAAATTCAGAAGCGACTGTCTGAGTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR213422 representing NM_001014012
  Red=Cloning site Green=Tags(s)
  
  MPHLGPLRCRTWAALLGQLLRPPRTVCNRAVGCHSQVAKAVLTSEQLKPHQQKPNFIIKVPKGTRDLSPQ
  QMVVREKILDKIISCFKRHGAKGLDTPAFELKEMLTEKYEDNFGLMYDLEDQGGELLSLRYDLTVPFARY
  LAMNKLKRMKRYQVGKVWRRESPAIVQGRYREFCQCDFDIAGEFDPMIPDAECLRIMCEILSGLQLGDFL
  IKVNDRRVVDGMFAVCGVPESKFRTICSSMDKLDKMSWEDVRLEMVAGKGLAPEVADRIGDYVQCHGGIS
  LVEELFKDPRLSQSQLALQGLGDLKLLFEYLRLFGIADKISLDLSLARGLDYYTGVIYEAVLLESPAQAG
  KETLNVGSVAAGGRYDRLVAQFDPKGHSVPCVGLSIGVERIFYLVEQKMKISCEKVRTTETQVFVATPQK
  NFLQERLKIIAQLWDAGIKAEMLYKNNPKLLTQLHYCEKEDIPLMVIIGEQEQNEGVVKLRSVASREEVT
  VNRESLVAEIQKRLSES
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001014012
• ORF Size: 1521 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001014012.2, NP_001014034.2
• RefSeq Size: 3089 bp
• RefSeq ORF: 1524 bp
• Locus ID: 307491
• Cytogenetics: 18p11
• MW: 57.3 kDa