Oplah (NM_053904) Rat Tagged ORF Clone

CAT#: RR213448

Oplah (Myc-DDK-tagged ORF) - Rat 5-oxoprolinase (ATP-hydrolysing) (Oplah), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Oplah
• Synonyms: MGC105359
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR213448 representing NM_053904
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAGCCCAGAAGGGCGCTTCCATTTCGCCATCGACCGCGGTGGCACCTTCACAGATGTCTTTGCCC
  AGTGCCCTGGAGGGCATGTGCGTGTCCTGAAGCTGCTCTCAGAGGACCCTGCCAACTATCCAGATGCACC
  CACAGAGGGCATCCGCCGAATTCTAGAGCAGGAGGAGGGTGTGCTGCTGCCTCGAGGCCGACCGCTAGAC
  ACCAGTCGCATTGCCAGCATCCGCATGGGTACCACGGTGGCCACCAATGCACTGTTGGAACGACAGGGAG
  AACGGGTGGCACTGCTGGTGACTCGGGGTTTCCGAGACCTGCTGCATATTGGCACTCAGGCCCGCCCGGA
  CCTCTTTGACTTGGCTGTGCCCATGCCAGAGGTTCTGTATGAGGAAGTGCTGGAGGTAGATGAGCGAGTG
  GTGCTGTATCGCGGAGAACCAGGTGCCGGCTCTCCTGTCAAAGGCCGCACAGGGGACCTGCTAGAGATAC
  AGCAGCCTGTGGACCTGGAAGCCCTGCGTGGGAAGCTGGAGGGGCTCTTGTCTCGGGGCATTCACAGTCT
  GGCAGTGGTGCTCATGCATTCGTACACGTGGGCCCAGCATGAGCAGCAGGTGGGCACGCTGGCCCGGGAG
  CTGGGCTTCACGCACGTCTCCTTGTCCTCGGAAGTCATGCCCATGGTACGAATTGTTCCTCGGGGCCATA
  CAGCCTGTGCTGACGCTTACCTTACTCCCACCATCCAGCGCTATGTGCAGGGCTTCCGCCGAGGCTTCCA
  GGGCCAGCTAAAGAATGTGCAAGTTCTCTTCATGCGCTCTGATGGTGGCCTCGCACCCATGGATGCTTTC
  AGTGGTTCCCGGGCTGTGCTCTCTGGCCCTGCTGGGGGTGTGGTTGGCTACTCAGCTACCACCTACCATC
  TGGAAGGCGGTCAGCCTGTCATTGGCTTTGACATGGGAGGCACATCCACAGACGTGAGCCGCTATGCTGG
  AGAATTTGAGCATGTCTTTGAGGCTAGCACAGCAGGCGTTACCCTTCAGGCACCCCAGTTGGACATCAAC
  ACAGTGGCAGCTGGCGGGGGTTCCCGCCTCTTCTTCAGATCTGGCCTCTTTGTGGTTGGTCCAGAGTCAG
  CAGGTGCCCACCCAGGTCCTGCCTGCTACCGTAAAGGGGGTCCTGTGACAGTGACAGATGCTAATCTGGT
  CCTGGGTCGCCTGCTGCCTGCCTCCTTCCCCTGCATTTTTGGGCCAGGAGAAGACCAGCCACTGTCTCCT
  GAGGCTTCCCGAAAGGCTCTAGAGGCTGTGGCCATGGAGGTCAACAGTTTCTTGACCAATGGACCGTGCC
  CAGCTTCCCAACTAAGTCTGGAAGAGGTGGCCATGGGGTTTGTGCGTGTTGCCAATGAAGCCATGTGCCG
  GCCTATCCGTGCCCTCACACAGGCACGAGGCCATGACCCCTCAGCCCATGTATTGGCTTGCTTTGGAGGA
  GCTGGTGGGCAACACGCTTGTGCCATTGCCCGGGCCCTGGGGATGGATACTGTGCACATTCACAGGCACA
  GCGGGCTGCTGTCAGCACTAGGACTGGCCTTGGCAGATGTGGTTCACGAAGCACAGGAGCCCTGTTCCCT
  GTCTTACACACCTGAAACCTTTGCACAACTGGACCAGAGACTGAGCCGCCTGGAGGAGCAGTGTGTGGAT
  GCCTTGCAGGTCCAGGGCTTCCCTAGGTCTCAGATCAGCACCGAGAGCTTCCTGCATCTTCGCTACCAAG
  GCACTGACTGCGCCCTAATGGTGTCTGCCCATCAGCATCCGGCCACAGCCTGCTCACCCCGAGCTGGTGA
  CTTTGGAGCCGCATTTGTGGAGAGGTACATGAGAGAGTTTGGCTTCATTATCCCCGAGCGGCCGGTGGTG
  GTAGATGATGTACGTGTGAGGGGAACTGGCCGTAGTGGACTTCAGCTGGAGGACACCCCCAAAATCCAGA
  CTGGACCTCCCCACGTGGAAAAGGTGACCCAGTGCTACTTTGAAGGGGGTTATCAGGAGACTCCCGTGTA
  CCTTTTAGGAGAACTAGGCTACGGGCACCAGCTCCAAGGGCCCTGCCTTATCATCGACAACAACAGCACC
  ATCCTTGTAGAACCGGGTTGCCAAGCAGAGGTGACTGATACAGGGGACATCCGCATTTCTGTGGGAGCTG
  AGGGTCCTAGTATGGCAGATACCAGGCTTGACCCCATCCAGCTGTCTATTTTCTCACACCGCTTCATGAG
  CATTGCTGAGCAGATGGGCCGCATCCTACAGCGCACAGCCATCTCTACCAACATCAAGGAACGCCTCGAC
  TTCTCCTGTGCCCTCTTTGGGCCAGATGGGGGCCTCGTCTCCAATGCTCCCCACATTCCTGTGCACCTGG
  GTGCCATGCAAGAGACTGTACAGTTCCAGATTCAGCACTTAGGAGCCGACCTCCATCCTGGTGATGTGTT
  GCTCAGCAACCATCCCAGCGCAGGGGGCAGCCATCTTCCTGACCTGACTGTCATTACACCGGTGTTTTGG
  CCAGGCCAGACGAGGCCTGTGTTCTACGTGGCTAGCCGAGGGCACCACGCAGACATTGGAGGAATCACAC
  CGGGCTCTATGCCGCCTCACTCCACCACGCTGCAACAGGAGGGTGCCGTTTTTCTGTCCTTCAAACTGGT
  CCAGGGAGGCGTCTTCCAGGAAGAGGCAGTGACAGAGGCCCTACGGGCACCAGGCAAGATCTCTGGCTGT
  AGTGGAACCAGGAACCTGCATGACAACCTGTCGGATCTTCGTGCCCAGGTGGCAGCTAACCAGAAAGGCA
  TCCAGCTGGTGGGAGAGCTGATCGGACAGTATGGCTTAGATGTGGTGCAGGCCTATATGGGCCATATTCA
  GGCGAATGCTGAGCTAGCAGTGAGAGACATGCTCCGGGCTTTTGGAACTTCCCGGCAGGCCAGGGGCCTG
  CCCCTGGAGGTGTCTGCAGAGGATCACATGGATGATGGCTCTCCCATCTGTCTGCGTGTTCAGATCAACC
  TGAGTCAGGGCAGTGCGGTATTTGACTTTACTGGTTCCGGGTCTGAGGTGTTTGGCAATCTCAATGCCCC
  GAGAGCCATAACACTGTCTGCTCTCATCTATTGCTTACGCTGTCTAGTGGGCCGTGACATCCCACTTAAC
  CAGGGTTGCCTGGCTCCTGTGCGTGTCATAATTCCCAAAGGCTCCATATTGGATCCATCCCCAGAGGCAG
  CAGTGGTCGGCGGCAACGTGCTCACATCTCAGCGAGTAGTGGATGTCATTCTGGGGGCTTTTGGGGCCTG
  TTCAGCCTCCCAGGGCTGCATGAACAATGTGACCCTGGGCAATGCCCGTATGGGCTACTATGAGACAGTG
  GCTGGTGGTGCCGGTGCGGGCCCTGGCTGGCATGGGCGCAGTGGTGTACACAGTCACATGACCAACACAC
  GCATTACGGATCCAGAGATTCTGGAGAGTCGGTATCCAGTTATCCTGCGCCGCTTTGAGCTGAGGCCAGG
  CTCCGGGGGCCGAGGTCGCTTCCGGGGAGGTGATGGCGTAGTCCGAGAGCTGGTCTTTCGGGAAGAGGCG
  CTGTTGTCTGTGCTCACCGAGCGCCGGGCCTTCCAGCCTTACGGCCTCCACGGGGGAGAGCCTGGTGCGC
  GTGGCTTAAACCTCCTGATCAGAAAAGATGGGCGCACAGTGAATTTGGGCGGCAAGACATCTGTGACCGT
  GTACCCCGGGGACGTGTTCTGCCTCCACACGCCTGGGGGTGGGGGCTACGGAGACCCGGAGGATCCAGCG
  CCACCACCAGGCTCGCCCCCGCTATTTCCAGCCTTCCCCGAGCGCGGCAGTGTATTCGAGTACCGCCGCG
  CCCAGGAAGCCGTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR213448 representing NM_053904
  Red=Cloning site Green=Tags(s)
  
  MGSPEGRFHFAIDRGGTFTDVFAQCPGGHVRVLKLLSEDPANYPDAPTEGIRRILEQEEGVLLPRGRPLD
  TSRIASIRMGTTVATNALLERQGERVALLVTRGFRDLLHIGTQARPDLFDLAVPMPEVLYEEVLEVDERV
  VLYRGEPGAGSPVKGRTGDLLEIQQPVDLEALRGKLEGLLSRGIHSLAVVLMHSYTWAQHEQQVGTLARE
  LGFTHVSLSSEVMPMVRIVPRGHTACADAYLTPTIQRYVQGFRRGFQGQLKNVQVLFMRSDGGLAPMDAF
  SGSRAVLSGPAGGVVGYSATTYHLEGGQPVIGFDMGGTSTDVSRYAGEFEHVFEASTAGVTLQAPQLDIN
  TVAAGGGSRLFFRSGLFVVGPESAGAHPGPACYRKGGPVTVTDANLVLGRLLPASFPCIFGPGEDQPLSP
  EASRKALEAVAMEVNSFLTNGPCPASQLSLEEVAMGFVRVANEAMCRPIRALTQARGHDPSAHVLACFGG
  AGGQHACAIARALGMDTVHIHRHSGLLSALGLALADVVHEAQEPCSLSYTPETFAQLDQRLSRLEEQCVD
  ALQVQGFPRSQISTESFLHLRYQGTDCALMVSAHQHPATACSPRAGDFGAAFVERYMREFGFIIPERPVV
  VDDVRVRGTGRSGLQLEDTPKIQTGPPHVEKVTQCYFEGGYQETPVYLLGELGYGHQLQGPCLIIDNNST
  ILVEPGCQAEVTDTGDIRISVGAEGPSMADTRLDPIQLSIFSHRFMSIAEQMGRILQRTAISTNIKERLD
  FSCALFGPDGGLVSNAPHIPVHLGAMQETVQFQIQHLGADLHPGDVLLSNHPSAGGSHLPDLTVITPVFW
  PGQTRPVFYVASRGHHADIGGITPGSMPPHSTTLQQEGAVFLSFKLVQGGVFQEEAVTEALRAPGKISGC
  SGTRNLHDNLSDLRAQVAANQKGIQLVGELIGQYGLDVVQAYMGHIQANAELAVRDMLRAFGTSRQARGL
  PLEVSAEDHMDDGSPICLRVQINLSQGSAVFDFTGSGSEVFGNLNAPRAITLSALIYCLRCLVGRDIPLN
  QGCLAPVRVIIPKGSILDPSPEAAVVGGNVLTSQRVVDVILGAFGACSASQGCMNNVTLGNARMGYYETV
  AGGAGAGPGWHGRSGVHSHMTNTRITDPEILESRYPVILRRFELRPGSGGRGRFRGGDGVVRELVFREEA
  LLSVLTERRAFQPYGLHGGEPGARGLNLLIRKDGRTVNLGGKTSVTVYPGDVFCLHTPGGGGYGDPEDPA
  PPPGSPPLFPAFPERGSVFEYRRAQEAV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053904
• ORF Size: 3864 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_053904.1, NP_446356.1
• RefSeq Size: 4003
• RefSeq ORF: 3867
• Locus ID: 116684
• MW: 137.7 kDa
• Gene Summary: catalyzes cleavage of 5-oxo-L-proline to form L-glutamate and is coupled to the hydrolysis of ATP to ADP and inorganic phosphate [RGD, Feb 2006]