Psmd12 (NM_001005875) Rat Tagged ORF Clone

CAT#: RR213611

Psmd12 (Myc-DDK-tagged ORF) - Rat proteasome (prosome, macropain) 26S subunit, non-ATPase, 12 (Psmd12), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Psmd12
• Synonyms: MGC94696
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR213611 representing NM_001005875
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGACGGCGGCTCGGAACGGGCCGATGGGCGCATTGTGAAGATGGAGGTGGACTACAGCGCCACAG
  TGGACCAGCGCCTGCCTGAGTGCGAGAAGCTGGCCAAGGAAGGACGGCTTCAGGAAGTCATCGAAACCCT
  TCTCTCTCTAGAAAAGCAGACCCGGACTGCCTCTGACATGGTGTCCACGTCCCGCATCTTGGTTGCAGTA
  GTGAAAATGTGCTATGAGGCCAAAGAGTGGGACTTACTTAATGAAAATATTATGCTTCTGTCAAAACGAA
  GGAGTCAGTTAAAACAAGCAGTTGCAAAAATGGTCCAGCAGTGCTGTACTTATGTGGAGGAGATCACAGA
  CCTGCCAGTCAAGCTGCGGTTGATTGATACCCTGCGTATGGTTACAGAAGGAAAGATTTATGTTGAAATT
  GAGCGTGCCAGGCTGACTAAAACCTTAGCAACTATAAAAGAGCAAAATGGCGACGTGAAGGAGGCCGCCT
  CCATCCTGCAGGAGTTACAGGTGGAAACCTATGGGTCTATGGAGAAGAAGGAGCGAGTGGAGTTTATTCT
  GGAGCAGATGAGGCTCTGCCTAGCCGTGAAGGATTACATTCGCACACAGATCATCAGCAAAAAAATTAAC
  ACCAAATTCTTCCAGGAAGAAAACACAGAGAAATTAAAGTTGAAGTACTATAACTTAATGATTCAGCTGG
  ATCAGCATGAGGGGTCCTATCTGTCCATCTGTAAGCACTACAGAGCCATCTACGACACTCCCTGCATTCA
  GGCAGAAAGTGACAAGTGGCAGCAGGCTCTGAAGAGTGTTGTTCTCTATGTTATCCTGGCTCCTTTTGAC
  AATGAGCAGTCAGATTTGGTTCACCGGATAAGTAGTGACAAGAAGCTGGAAGAGATTCCCAAATACAAGG
  ATCTGCTGAAGCTTTTTACCACAATGGAGCTGATGCGCTGGTCCACACTTGTTGAGGACTATGGGGTGGA
  GCTACGGAAGGGCTCCTCGGAGACCCCAGCAACTGACGTCTTCAGTTCTACAGAAGAAGGTGAAAAACGG
  TGGAAAGACTTGAAGAACAGAGTCGTGGAGCATAACATCAGAATTATGGCCAAGTACTACACTCGGATAA
  CCATGAAAAGGATGGCACAACTTCTGGATCTCTCTGTAGATGAGTCAGAGGCCTTCCTCTCGAATCTAGT
  CGTTAACAAGACCATCTTTGCTAAAGTGGACAGGTTGGCTGGGGTCATCAACTTCCAGAGACCCAAGGAT
  CCAAATAACTTATTAAATGACTGGTCTCAGAAACTGAATTCACTGATGTCTCTGGTGAACAAAACGACAC
  ACCTCATAGCCAAAGAGGAGATGATACATAATCTACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR213611 representing NM_001005875
  Red=Cloning site Green=Tags(s)
  
  MADGGSERADGRIVKMEVDYSATVDQRLPECEKLAKEGRLQEVIETLLSLEKQTRTASDMVSTSRILVAV
  VKMCYEAKEWDLLNENIMLLSKRRSQLKQAVAKMVQQCCTYVEEITDLPVKLRLIDTLRMVTEGKIYVEI
  ERARLTKTLATIKEQNGDVKEAASILQELQVETYGSMEKKERVEFILEQMRLCLAVKDYIRTQIISKKIN
  TKFFQEENTEKLKLKYYNLMIQLDQHEGSYLSICKHYRAIYDTPCIQAESDKWQQALKSVVLYVILAPFD
  NEQSDLVHRISSDKKLEEIPKYKDLLKLFTTMELMRWSTLVEDYGVELRKGSSETPATDVFSSTEEGEKR
  WKDLKNRVVEHNIRIMAKYYTRITMKRMAQLLDLSVDESEAFLSNLVVNKTIFAKVDRLAGVINFQRPKD
  PNNLLNDWSQKLNSLMSLVNKTTHLIAKEEMIHNLQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001005875
• ORF Size: 1368 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001005875.1, NP_001005875.1
• RefSeq Size: 1512 bp
• RefSeq ORF: 1371 bp
• Locus ID: 287772
• Cytogenetics: 10q32.1
• MW: 52.9 kDa