Smc3 (NM_031583) Rat Tagged ORF Clone

CAT#: RR213612

Smc3 (Myc-DDK-tagged ORF) - Rat structural maintenance of chromosomes 3 (Smc3), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Smc3
• Synonyms: bamacan; Cspg6; SMC-3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR213612 representing NM_031583
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACATCAAGCAGGTGATTATCCAGGGCTTCCGAAGTTACCGAGATCAAACAATTGTAGATCCCTTCA
  GTTCAAAACATAATGTTATTGTGGGCAGAAATGGATCTGGAAAAAGCAACTTTTTTTATGCAATCCAGTT
  TGTTCTCAGTGATGAATTTAGTCATCTTCGTCCAGAGCAGCGATTGGCTTTGTTGCACGAGGGTACAGGT
  CCTCGTGTTATTTCTGCTTTTGTGGAAATCATTTTTGACAATTCAGACAACCGGTTACCAATTGATAAAG
  AGGAAGTTTCGCTTCGAAGAGTTATTGGTGCCAAAAAAGATCAGTATTTCCTAGATAAGAAGATGGTCAC
  GAAAAATGATGTGATGAATCTCCTTGAAAGTGCTGGGTTTTCGAGAAGTAATCCTTATTACATTGTTAAA
  CAAGGAAAGATCAACCAAATGGCAACAGCACCGGATTCTCAGAGATTAAAGCTGTTGAGAGAAGTAGCTG
  GCACTCGAGTGTATGACGAGCGCAAAGAGGAAAGCATCTCCCTGATGAAAGAAACAGAGGGCAAACGGGA
  AAAGATCAATGAGTTGTTAAAATACATTGAAGAGCGATTACATACTCTAGAGGAGGAGAAGGAGGAACTG
  GCCCAGTATCAGAAGTGGGATAAAATGAGGCGTGCCCTGGAGTACACCATCTACAACCAGGAGCTCAACG
  AGACTCGCGCCAAGCTTGATGAGCTTTCTGCTAAGCGAGAAACAAGCGGAGAGAAATCCAGACAATTAAG
  AGATGCCCAGCAGGATGCAAGAGATAAAATGGAGGATATTGAGCGCCAAGTTAGAGAACTGAAAACAAAA
  ATTTCAGCCATGAAAGAAGAAAAAGAACAGCTCAGTGCGGAGAGACAAGAGCAGATTAAGCAAAGGACCA
  AGCTGGAGCTTAAAGCCAAGGACTTACAGGATGAGCTGGCAGGGAACAGTGAGCAGCGGAAACGTTTATT
  AAAAGAAAGACAGAAGCTGCTTGAAAAAATAGAAGAAAAGCAGAAAGAACTGGCAGAAACAGAACCGAAA
  TTCAACAGCGTAAAGGAAAAAGAAGAGCGAGGAATTGCTAGATTGGCTCAAGCTACACAGGAAAGAACTG
  ATCTTTATGCAAAGCAGGGTCGAGGAAGCCAGTTTACATCAAAAGAAGAAAGAGATAAGTGGATTAAGAA
  GGAACTGAAGTCTTTAGATCAGGCTATTAATGATAAGAAAAGACAGATTGCTGCTATACACAAGGATTTG
  GAGGATACCGAGGCCAATAAAGAGAAAAATCTGGAGCAATATAATAAACTGGATCAGGATCTCAATGAAG
  TCAAAGCTCGAGTTGAAGAACTGGACAGAAAATATTATGAAGTGAAAAATAAGAAAGATGAACTACAAAG
  TGAAAGAAATTACTTGTGGAGAGAGGAGAATGCAGAACAGCAAGCACTTGCCGCTAAGAGAGAAGACCTT
  GAGAAGAAGCAGCAGCTTCTTAGAGCGGCAACAGGAAAGGCCATTTTAAATGGAATCGACAGCATTAACA
  AAGTGTTAGACCATTTCCGGCGGAAGGGTATAAACCAGCATGTGCAAAATGGCTACCATGGCATCGTGAT
  GAATAACTTTGAGTGCGAACCAGCTTTCTATACTTGTGTGGAAGTCACTGCCGGTAACAGGTTATTCTAT
  CACATTGTCGATTCAGATGAAGTCAGCACGAAGATCTTAATGGAGTTCAATAAAATGAATCTTCCTGGAG
  AGGTGACTTTCCTGCCTCTTAACAAGTTAGATGTGAGGGACACTGCCTATCCTGAAACTAACGATGCTAT
  TCCTATGATCAGTAAGCTGAGGTACAATCCCAGATTCGACAAAGCTTTCAAACATGTGTTTGGAAAGACA
  CTCATCTGTCGGAGCATGGAGGTTTCCACTCAGCTAGCTCGTGCCTTCACTATGGACTGCATTACTCTGG
  AAGGTGATCAAGTCAGCCATCGAGGTGCTCTGACTGGAGGCTATTACGACACAAGAAAGTCTCGCCTTGA
  GTTACAGAAGGATGTTAGGAAAGCAGAGGAGGAGCTGGGTGAGCTCGAAGCTAAGCTCAATGAAAACCTG
  CGCAGAAACATTGAAAGGATTAATAATGAAATTGATCAATTGATGAACCAAATGCAGCAGATAGAGACCC
  AACAGAGAAAATTTAAAGCATCCAGAGATAGCATCTTATCAGAGATGAAGATGCTAAAAGAGAAGAGACA
  GCAGTCAGAAAAGACCTTCATGCCAAAGCAACGCAGTTTGCAGAGTCTGGAGGCCAGTCTGCACGCTATG
  GAGTCGACCAGAGAGTCGCTGAAGGCAGAGCTAGGGACAGATTTGCTCTCTCAGCTCAGCCTGGAAGACC
  AGAAAAGAGTCGACGCACTGAATGACGAGATCCGGCAGCTTCAGCAGGAAAACAGACAGCTGCTGAATGA
  AAGAATTAAACTAGAAGGCATTATTACTCGAGTAGAGACTTACCTCAATGAGAATCTGAGGAAACGTCTG
  GACCAAGTAGAGCAGGAACTTAATGAACTGAGAGAGACAGAAGGTGGCACTGTTCTTACTGCCACCACGT
  CAGAACTTGAAGCTATTAATAAAAGAGTGAAAGATACTATGGCAAGATCAGAAGATTTGGATAATTCCAT
  TGACAAAACAGAAGCTGGAATTAAAGAGCTCCAGAAAAGCATGGAGCGCTGGAAGAACATGGAGAAGGAG
  CACATGGATGCCATAAATCACGACACTAAAGAGCTGGAGAAGATGACCAACCGACAGGGCATGCTGTTGA
  AGAAGAAAGAGGAGTGCATGAAGAAGATCCGAGAGCTGGGGTCCCTTCCCCAGGAAGCATTTGAAAAGTA
  CCAGACACTGAGCCTGAAGCAGTTGTTTCGGAAACTTGAGCAGTGCAACACAGAGTTGAAGAAGTACAGC
  CATGTCAACAAGAAGGCTCTGGACCAGTTTGTGAATTTCTCTGAGCAGAAGGAGAAACTGATAAAGCGAC
  AGGAAGAATTGGACAGGGGCTACAAGTCAATCATGGAGTTGATGAATGTACTCGAACTTAGAAAATATGA
  AGCTATCCAGTTAACCTTCAAACAGGTATCTAAGAACTTTAGTGAAGTTTTCCAGAAGTTGGTACCTGGG
  GGAAAAGCGACCTTGGTGATGAAGAAAGGAGACGTGGAGGGCAGCCAGTCTCAGGACGAAGGAGAGGGGA
  GCGGCGAGAGCGAGAGGGGCTCTGGGTCACAAAGCAGCGTTCCATCAGTTGACCAGTTCACAGGAGTCGG
  GATCAGGGTATCATTTACAGGAAAGCAAGGCGAGATGAGAGAAATGCAGCAGCTTTCAGGAGGGCAGAAG
  TCTCTGGTAGCCCTTGCTCTCATTTTTGCCATTCAGAAGTGTGACCCTGCTCCTTTCTACCTGTTTGATG
  AGATCGACCAAGCTTTGGATGCTCAGCACAGAAAAGCTGTGTCAGATATGATTATGGAACTAGCTGTACA
  CGCCCAGTTTATCACTACCACTTTTAGGCCTGAACTGCTTGAGTCTGCTGATAAATTCTACGGTGTAAAG
  TTCAGAAATAAGGTTAGTCACATCGACGTGATCACAGCAGAGATGGCCAAAGACTTTGTAGAAGACGATA
  CCACGCATGGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR213612 representing NM_031583
  Red=Cloning site Green=Tags(s)
  
  MYIKQVIIQGFRSYRDQTIVDPFSSKHNVIVGRNGSGKSNFFYAIQFVLSDEFSHLRPEQRLALLHEGTG
  PRVISAFVEIIFDNSDNRLPIDKEEVSLRRVIGAKKDQYFLDKKMVTKNDVMNLLESAGFSRSNPYYIVK
  QGKINQMATAPDSQRLKLLREVAGTRVYDERKEESISLMKETEGKREKINELLKYIEERLHTLEEEKEEL
  AQYQKWDKMRRALEYTIYNQELNETRAKLDELSAKRETSGEKSRQLRDAQQDARDKMEDIERQVRELKTK
  ISAMKEEKEQLSAERQEQIKQRTKLELKAKDLQDELAGNSEQRKRLLKERQKLLEKIEEKQKELAETEPK
  FNSVKEKEERGIARLAQATQERTDLYAKQGRGSQFTSKEERDKWIKKELKSLDQAINDKKRQIAAIHKDL
  EDTEANKEKNLEQYNKLDQDLNEVKARVEELDRKYYEVKNKKDELQSERNYLWREENAEQQALAAKREDL
  EKKQQLLRAATGKAILNGIDSINKVLDHFRRKGINQHVQNGYHGIVMNNFECEPAFYTCVEVTAGNRLFY
  HIVDSDEVSTKILMEFNKMNLPGEVTFLPLNKLDVRDTAYPETNDAIPMISKLRYNPRFDKAFKHVFGKT
  LICRSMEVSTQLARAFTMDCITLEGDQVSHRGALTGGYYDTRKSRLELQKDVRKAEEELGELEAKLNENL
  RRNIERINNEIDQLMNQMQQIETQQRKFKASRDSILSEMKMLKEKRQQSEKTFMPKQRSLQSLEASLHAM
  ESTRESLKAELGTDLLSQLSLEDQKRVDALNDEIRQLQQENRQLLNERIKLEGIITRVETYLNENLRKRL
  DQVEQELNELRETEGGTVLTATTSELEAINKRVKDTMARSEDLDNSIDKTEAGIKELQKSMERWKNMEKE
  HMDAINHDTKELEKMTNRQGMLLKKKEECMKKIRELGSLPQEAFEKYQTLSLKQLFRKLEQCNTELKKYS
  HVNKKALDQFVNFSEQKEKLIKRQEELDRGYKSIMELMNVLELRKYEAIQLTFKQVSKNFSEVFQKLVPG
  GKATLVMKKGDVEGSQSQDEGEGSGESERGSGSQSSVPSVDQFTGVGIRVSFTGKQGEMREMQQLSGGQK
  SLVALALIFAIQKCDPAPFYLFDEIDQALDAQHRKAVSDMIMELAVHAQFITTTFRPELLESADKFYGVK
  FRNKVSHIDVITAEMAKDFVEDDTTHG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031583
• ORF Size: 3651 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_031583.2, NP_113771.2
• RefSeq Size: 4158
• RefSeq ORF: 3654
• Locus ID: 29486
• MW: 141.5 kDa
• Gene Summary: core protein of a chondroitin sulfate proteoglycan that is a component of basement membranes [RGD, Feb 2006]