Nfasc (NM_053909) Rat Tagged ORF Clone

CAT#: RR214024

Nfasc (Myc-DDK-tagged ORF) - Rat neurofascin (Nfasc), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nfasc
• Synonyms: NF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214024 representing NM_053909
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGGCAGCAGGCGCCACCCTGGGTCCACGTAGCCCTCATCCTCTTCCTCCTCAGCCTCGGAGGGG
  CCATTGAGATTCCGATGGATCTGACCCAACCCCCAACGATCACCAAGCAGTCGGTGAAGGACCACATCGT
  GGACCCCCGAGATAACATCCTGATTGAATGTGAAGCTAAAGGGAACCCCGCCCCCAGTTTCCACTGGACT
  CGCAACAGCAGGTTCTTCAACATTGCCAAGGACCCACGGGTGTCCATGAGGAGGAGGTCTGGGACCTTGG
  TGATCGACTTCCGCAGTGGTGGGCGGCCTGAGGAGTACGAAGGGGAGTACCAGTGCTTTGCCCGGAACAA
  ATTCGGCACAGCTCTTAGCAACCGCATCCGCCTGCAGGTGTCCAAATCTCCCCTGTGGCCCAAGGAAAAC
  CTAGACCCCGTCGTGGTTCAAGAGGGTGCCCCCTTAACCCTGCAGTGCAACCCCCCACCTGGCCTCCCAT
  CCCCCGTCATCTTCTGGATGAGCAGCTCCATGGAGCCCATCACCCAGGACAAGCGTGTCTCCCAGGGTCA
  CAACGGGGACCTGTACTTCTCCAACGTCATGCTGCAGGACATGCAGACCGACTACAGCTGCAATGCACGC
  TTCCACTTCACCCACACCATTCAGCAGAAGAATCCCTTCACCCTCAAAGTCCTCACCAACAACCCCTATA
  ATGACTCGTCTTTAAGAAACCACCCTGACATATATAGTGCCCGAGGAGTTGCGGAAAGAACACCTAGCTT
  CATGTATCCCCAGGGCACGTCAAGCAGTCAGATGGTACTGCGCGGCATGGACCTGCTGCTGGAGTGCATT
  GCCTCTGGGGTCCCAACACCAGATATTGCATGGTACAAGAAAGGTGGGGACCTCCCATCTGACAAGGCCA
  AGTTCGAGAATTTTAACAAGGCTCTGCGCATCACCAATGTCTCTGAAGAGGACTCTGGGGAGTATTTCTG
  CCTGGCCTCCAACAAGATGGGCAGCATCCGGCACACGATCTCGGTGAGAGTAAAGGCTGCCCCATACTGG
  CTGGATGAGCCCAAGAACTTGATCCTGGCTCCTGGTGAAGATGGGAGGCTGGTGTGTCGAGCCAATGGGA
  ACCCGAAGCCGACCGTCCAGTGGCTGGTGAATGGAGACCCTTTGCAATCGGCACCACCCAACCCCAACCG
  TGAGGTGGCCGGAGACACTATCATCTTCCGGGACACTCAGATCAGCAGCAGGGCAGTGTACCAGTGTAAC
  ACATCCAACGAACATGGCTACCTGCTGGCCAATGCCTTCGTCAGCGTATTAGATGTACCCCCTCGGATGC
  TGTCTCCCCGGAACCAGCTCATCAGGGTGATCCTTTACAACCGGACGCGACTGGACTGTCCGTTCTTTGG
  GTCTCCCATTCCAACACTCCGATGGTTTAAGAATGGGCAAGGAAGCAACCTGGATGGTGGTAACTACCAC
  GTCTACGAAAATGGCAGCCTGGAAATCAAGATGATTCGCAAAGAGGACCAAGGCATCTACACCTGTGTGG
  CCACCAACATCCTGGGAAAAGCTGAAAATCAAGTCCGCCTGGAGGTCAAAGACCCCACCAGGATCTACAG
  GATGCCTGAAGACCAGGTGGCCAAGAGGGGCACCACAGTGCAGCTGGAGTGCCGTGTGAAGCATGACCCC
  TCCTTGAAACTCACAGTCTCCTGGCTGAAGGATGACGAGCCACTCTACATTGGAAACAGGATGAAAAAGG
  AAGATGACTCCCTGACCATCTTCGGAGTGGCAGAGCGGGACCAAGGCAGTTACACGTGCATGGCCAGCAC
  CGAGCTGGACCAGGACCTGGCAAAGGCCTACCTCACTGTTCTAGGGCGGCCAGACCGACCCAGGGACCTG
  GAGCTCACTGACCTGGCGGAAAGGAGTGTGAGGCTGACCTGGATCCCGGGGGATGATAACAACAGTCCCA
  TCACAGACTACGTTGTCCAGTTTGAAGAGGACCAGTTCCAGCCAGGAGTCTGGCATGACCACTCCAAGTT
  CCCAGGCAGTGTCAACTCAGCCGTCCTCCATCTGTCCCCGTATGTCAACTATCAGTTCCGAGTCATCGCT
  GTCAACGAGGTTGGGAGCAGCCACCCCAGCCTTCCATCCGAGCGGTACCGAACAAGCGGGGCACCCCCTG
  AATCCAACCCCAGTGATGTGAAGGGCGAAGGGACACGAAAAAACAATATGGAGATCACATGGACGCCTAT
  GAATGCTACCTCCGCCTTTGGCCCCAACCTGCGCTACATTGTCAAGTGGCGACGGAGAGAAACCCGAGAG
  ACTTGGAACAATGTCACCGTGTGGGGCTCTCGCTATGTGGTGGGGCAGACCCCTGTCTACGTACCCTATG
  AGATCCGAGTCCAGGCTGAAAATGACTTTGGGAAAGGCCCGGAGCCTGAAACCGTCATTGGGTACTCGGG
  GGAAGATTATCCCAGGGCTGCACCCACTGAAGTTAAAATCCGAGTCCTGAACAGCACAGCCATCAGCCTT
  CAGTGGAACCGCGTCTACCCTGACACGGTCCAGGGCCAGCTCAGAGAGTATCGAGCTTACTACTGGAGGG
  AAAGCAGTTTGCTGAAGAACCTGTGGGTGTCTCAGAAGAGACAGCAGGCCAGCTTCCCTGGCGACCGCCC
  CCGGGGCGTGGTGGGTCGCCTGTTCCCCTACAGTAACTACAAGCTGGAGATGGTTGTGGTCAATGGGAGA
  GGTGATGGGCCTCGAAGTGAAACCAAGGAATTCACCACCCCGGAAGGAGTACCCAGTGCCCCCAGGCGTT
  TCAGAGTCCGACAGCCCAACCTGGAGACCATCAACCTGGAATGGGATCACCCAGAGCACCCCAACGGGAT
  CCTGATTGGATACACGCTCAGATACGTGCCCTTTAATGGAACCAAACTGGGAAAGCAGATGGTGGAAAAC
  TTCTCTCCCAATCAGACCAAGTTCTCGGTGCAGAGAGCAGACCCCGTGTCCCGTTACCGCTTCTCCCTCA
  GTGCCAGGACGCAGGTGGGCTCTGGAGAAGCAGCCACGGAGGAGTCCCCAACACCTCCAAATGAAGCTAC
  TCCAACTGCAGCTTACACCAACAACCAGACTGACATCGCCACCCAGGGCTGGTTCATCGGGCTCATGTGT
  GCCATTGCCCTTCTGGTGCTGATCCTGCTGATCGTCTGCTTCATCAAGAGGAGTCGTGGCGGCAAGTACC
  CAGTGCGAGAAAAGAAGGATGTCCCCTTGGGCCCTGAAGACCCCAAAGAAGAAGATGGTTCATTTGACTA
  CAGTGACGAGGACAACAAGCCCCTGCAGGGCAGCCAGACATCTCTGGACGGCACCATCAAGCAGCAGGAG
  AGTGACGACAGCCTAGTGGACTACGGCGAGGGTGGCGAGGGCCAGTTCAACGAAGATGGCTCCTTTATTG
  GCCAGTACACTGTCAGAAAGGACAAGGAGGAGACCGAGGGCAATGAGAGCTCAGAGGCCACATCTCCAGT
  CAATGCCATCTATTCCCTGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214024 representing NM_053909
  Red=Cloning site Green=Tags(s)
  
  MARQQAPPWVHVALILFLLSLGGAIEIPMDLTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWT
  RNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKEN
  LDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNAR
  FHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECI
  ASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYW
  LDEPKNLILAPGEDGRLVCRANGNPKPTVQWLVNGDPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCN
  TSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYH
  VYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDP
  SLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLGRPDRPRDL
  ELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLHLSPYVNYQFRVIA
  VNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRE
  TWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPETVIGYSGEDYPRAAPTEVKIRVLNSTAISL
  QWNRVYPDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVGRLFPYSNYKLEMVVVNGR
  GDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLRYVPFNGTKLGKQMVEN
  FSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPTPPNEATPTAAYTNNQTDIATQGWFIGLMC
  AIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQE
  SDDSLVDYGEGGEGQFNEDGSFIGQYTVRKDKEETEGNESSEATSPVNAIYSLA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053909
• ORF Size: 3522 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_053909.1, NM_053909.2, NP_446361.1
• RefSeq Size: 9627
• RefSeq ORF: 3525
• Locus ID: 116690
• MW: 132.2 kDa
• Gene Summary: This gene encodes an L1 family immunoglobulin cell adhesion molecule with multiple IGcam and fibronectin domains. The protein functions in neurite outgrowth, neurite fasciculation, and organization of the axon initial segment (AIS) and nodes of Ranvier on axons during early development. Both the AIS and nodes of Ranvier contain high densities of voltage-gated Na+ (Nav) channels which are clustered by interactions with cytoskeletal and scaffolding proteins including this protein, gliomedin, ankyrin 3 (ankyrin-G), and betaIV spectrin. This protein links the AIS extracellular matrix to the intracellular cytoskeleton. This gene undergoes extensive alternative splicing, and the full-length nature of some variants has not been determined. [provided by RefSeq, May 2009]