Nfasc (NM_053909) Rat Untagged Clone

CAT#: RN214024

Nfasc (untagged ORF) - Rat neurofascin (Nfasc), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Nfasc
• Synonyms: NF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN214024 representing NM_053909
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGGCAGCAGGCGCCACCCTGGGTCCACGTAGCCCTCATCCTCTTCCTCCTCAGCCTCGGAGGGG
  CCATTGAGATTCCGATGGATCTGACCCAACCCCCAACGATCACCAAGCAGTCGGTGAAGGACCACATCGT
  GGACCCCCGAGATAACATCCTGATTGAATGTGAAGCTAAAGGGAACCCCGCCCCCAGTTTCCACTGGACT
  CGCAACAGCAGGTTCTTCAACATTGCCAAGGACCCACGGGTGTCCATGAGGAGGAGGTCTGGGACCTTGG
  TGATCGACTTCCGCAGTGGTGGGCGGCCTGAGGAGTACGAAGGGGAGTACCAGTGCTTTGCCCGGAACAA
  ATTCGGCACAGCTCTTAGCAACCGCATCCGCCTGCAGGTGTCCAAATCTCCCCTGTGGCCCAAGGAAAAC
  CTAGACCCCGTCGTGGTTCAAGAGGGTGCCCCCTTAACCCTGCAGTGCAACCCCCCACCTGGCCTCCCAT
  CCCCCGTCATCTTCTGGATGAGCAGCTCCATGGAGCCCATCACCCAGGACAAGCGTGTCTCCCAGGGTCA
  CAACGGGGACCTGTACTTCTCCAACGTCATGCTGCAGGACATGCAGACCGACTACAGCTGCAATGCACGC
  TTCCACTTCACCCACACCATTCAGCAGAAGAATCCCTTCACCCTCAAAGTCCTCACCAACAACCCCTATA
  ATGACTCGTCTTTAAGAAACCACCCTGACATATATAGTGCCCGAGGAGTTGCGGAAAGAACACCTAGCTT
  CATGTATCCCCAGGGCACGTCAAGCAGTCAGATGGTACTGCGCGGCATGGACCTGCTGCTGGAGTGCATT
  GCCTCTGGGGTCCCAACACCAGATATTGCATGGTACAAGAAAGGTGGGGACCTCCCATCTGACAAGGCCA
  AGTTCGAGAATTTTAACAAGGCTCTGCGCATCACCAATGTCTCTGAAGAGGACTCTGGGGAGTATTTCTG
  CCTGGCCTCCAACAAGATGGGCAGCATCCGGCACACGATCTCGGTGAGAGTAAAGGCTGCCCCATACTGG
  CTGGATGAGCCCAAGAACTTGATCCTGGCTCCTGGTGAAGATGGGAGGCTGGTGTGTCGAGCCAATGGGA
  ACCCGAAGCCGACCGTCCAGTGGCTGGTGAATGGAGACCCTTTGCAATCGGCACCACCCAACCCCAACCG
  TGAGGTGGCCGGAGACACTATCATCTTCCGGGACACTCAGATCAGCAGCAGGGCAGTGTACCAGTGTAAC
  ACATCCAACGAACATGGCTACCTGCTGGCCAATGCCTTCGTCAGCGTATTAGATGTACCCCCTCGGATGC
  TGTCTCCCCGGAACCAGCTCATCAGGGTGATCCTTTACAACCGGACGCGACTGGACTGTCCGTTCTTTGG
  GTCTCCCATTCCAACACTCCGATGGTTTAAGAATGGGCAAGGAAGCAACCTGGATGGTGGTAACTACCAC
  GTCTACGAAAATGGCAGCCTGGAAATCAAGATGATTCGCAAAGAGGACCAAGGCATCTACACCTGTGTGG
  CCACCAACATCCTGGGAAAAGCTGAAAATCAAGTCCGCCTGGAGGTCAAAGACCCCACCAGGATCTACAG
  GATGCCTGAAGACCAGGTGGCCAAGAGGGGCACCACAGTGCAGCTGGAGTGCCGTGTGAAGCATGACCCC
  TCCTTGAAACTCACAGTCTCCTGGCTGAAGGATGACGAGCCACTCTACATTGGAAACAGGATGAAAAAGG
  AAGATGACTCCCTGACCATCTTCGGAGTGGCAGAGCGGGACCAAGGCAGTTACACGTGCATGGCCAGCAC
  CGAGCTGGACCAGGACCTGGCAAAGGCCTACCTCACTGTTCTAGGGCGGCCAGACCGACCCAGGGACCTG
  GAGCTCACTGACCTGGCGGAAAGGAGTGTGAGGCTGACCTGGATCCCGGGGGATGATAACAACAGTCCCA
  TCACAGACTACGTTGTCCAGTTTGAAGAGGACCAGTTCCAGCCAGGAGTCTGGCATGACCACTCCAAGTT
  CCCAGGCAGTGTCAACTCAGCCGTCCTCCATCTGTCCCCGTATGTCAACTATCAGTTCCGAGTCATCGCT
  GTCAACGAGGTTGGGAGCAGCCACCCCAGCCTTCCATCCGAGCGGTACCGAACAAGCGGGGCACCCCCTG
  AATCCAACCCCAGTGATGTGAAGGGCGAAGGGACACGAAAAAACAATATGGAGATCACATGGACGCCTAT
  GAATGCTACCTCCGCCTTTGGCCCCAACCTGCGCTACATTGTCAAGTGGCGACGGAGAGAAACCCGAGAG
  ACTTGGAACAATGTCACCGTGTGGGGCTCTCGCTATGTGGTGGGGCAGACCCCTGTCTACGTACCCTATG
  AGATCCGAGTCCAGGCTGAAAATGACTTTGGGAAAGGCCCGGAGCCTGAAACCGTCATTGGGTACTCGGG
  GGAAGATTATCCCAGGGCTGCACCCACTGAAGTTAAAATCCGAGTCCTGAACAGCACAGCCATCAGCCTT
  CAGTGGAACCGCGTCTACCCTGACACGGTCCAGGGCCAGCTCAGAGAGTATCGAGCTTACTACTGGAGGG
  AAAGCAGTTTGCTGAAGAACCTGTGGGTGTCTCAGAAGAGACAGCAGGCCAGCTTCCCTGGCGACCGCCC
  CCGGGGCGTGGTGGGTCGCCTGTTCCCCTACAGTAACTACAAGCTGGAGATGGTTGTGGTCAATGGGAGA
  GGTGATGGGCCTCGAAGTGAAACCAAGGAATTCACCACCCCGGAAGGAGTACCCAGTGCCCCCAGGCGTT
  TCAGAGTCCGACAGCCCAACCTGGAGACCATCAACCTGGAATGGGATCACCCAGAGCACCCCAACGGGAT
  CCTGATTGGATACACGCTCAGATACGTGCCCTTTAATGGAACCAAACTGGGAAAGCAGATGGTGGAAAAC
  TTCTCTCCCAATCAGACCAAGTTCTCGGTGCAGAGAGCAGACCCCGTGTCCCGTTACCGCTTCTCCCTCA
  GTGCCAGGACGCAGGTGGGCTCTGGAGAAGCAGCCACGGAGGAGTCCCCAACACCTCCAAATGAAGCTAC
  TCCAACTGCAGCTTACACCAACAACCAGACTGACATCGCCACCCAGGGCTGGTTCATCGGGCTCATGTGT
  GCCATTGCCCTTCTGGTGCTGATCCTGCTGATCGTCTGCTTCATCAAGAGGAGTCGTGGCGGCAAGTACC
  CAGTGCGAGAAAAGAAGGATGTCCCCTTGGGCCCTGAAGACCCCAAAGAAGAAGATGGTTCATTTGACTA
  CAGTGACGAGGACAACAAGCCCCTGCAGGGCAGCCAGACATCTCTGGACGGCACCATCAAGCAGCAGGAG
  AGTGACGACAGCCTAGTGGACTACGGCGAGGGTGGCGAGGGCCAGTTCAACGAAGATGGCTCCTTTATTG
  GCCAGTACACTGTCAGAAAGGACAAGGAGGAGACCGAGGGCAATGAGAGCTCAGAGGCCACATCTCCAGT
  CAATGCCATCTATTCCCTGGCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_053909
• ORF Size: 3525 bp
• Insert Size: 3525
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_053909.2, NP_446361.1
• RefSeq Size: 9627
• RefSeq ORF: 3525
• Locus ID: 116690
• Gene Summary: This gene encodes an L1 family immunoglobulin cell adhesion molecule with multiple IGcam and fibronectin domains. The protein functions in neurite outgrowth, neurite fasciculation, and organization of the axon initial segment (AIS) and nodes of Ranvier on axons during early development. Both the AIS and nodes of Ranvier contain high densities of voltage-gated Na+ (Nav) channels which are clustered by interactions with cytoskeletal and scaffolding proteins including this protein, gliomedin, ankyrin 3 (ankyrin-G), and betaIV spectrin. This protein links the AIS extracellular matrix to the intracellular cytoskeleton. This gene undergoes extensive alternative splicing, and the full-length nature of some variants has not been determined. [provided by RefSeq, May 2009]
  Transcript Variant: This variant (3) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. The resulting isoform (3) includes the third fibronectin type 3 (FNIII) repeat, lacks the mucin-like domain, and has several differences in the N-terminal and central regions, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.