Polr3c (NM_001012081) Rat Tagged ORF Clone

CAT#: RR214037

  • TrueORF®

Polr3c (Myc-DDK-tagged ORF) - Rat polymerase (RNA) III (DNA directed) polypeptide C (62kD) (Polr3c), (10 ug)


  "NM_001012081" in other vectors (3)

Reconstitution Protocol

USD 720.00

5 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Symbol Polr3c
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR214037 representing NM_001012081
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACTCAAGCAGAAATTAAACTGTGCTCTTTGTTGCTACAAGAGCATTTTGGTGAGATTGTAGAAAAAA
TTGGAGTCCACCTAATCAGAACTGGGAGCCAGCCACTTAGAGTAATTGCCCATGACACAAAAGCATCACT
GGACCAGGTGAAAAAAGCCCTTTGTGTCCTCATTCACCATAACCTGGTCATCTATCATGTGCATAAACGC
GGTGTGGTGGAGTATGAAGCCCAATGCAGCCGGGTACTGCGGATGCTTCGGTACCCCCGGTACATCTATA
CTACCAAAACACTGTATGGTGACACTGGAGAGCTGATTGTTGAGGAGCTTCTGCTGAATGGCAAAATGAC
AATGTCGGCTGTTGTGAAGAAAGTAGCAGACCGACTCACAGAGACCATGGAGGATGGCAAGACCATGGAC
TACGCTGAGGTGTCAAATGCATTTGTTCGACTGGCAGATACTCACTTTGTACAGCGCTGTCCCCTGGTTC
CTGACACTGACAGTTCTGACCCCGGGCCGCCGCCACCTGCCCCAAACCTTGTCATTAATGAAAAGGACAT
GTACCTAGTTCCCAAACTGAGCTTGATAGGAAAAGGTAAGAGGAGGAGATCATCTGACGAAGATGCTGCT
GGAGAGCCCAAGGCCAAGAAACCAAGATGCACAGATAATGAGGAGCCCACACCAGACGATGGGATTTATT
GGCAAGTCAACCTGGACAGATTCCACCAGCACTTCCGTGACCAAGCAATTGTGAGCGCAGTGGCAAACCG
AATGGACCAGACAAGCAGCGAGATTGTGCGGACAATGCTCCGAATGAGTGAGATTACCACTCCCTCTGGT
GCGCCGTTTACTCAGCCACTGTCCTCCAATGAGATCTTCAGATCCCTGCCCGTCGGATATAATATCTCTA
AACAGGTTCTGGATCAGTACCTCACGCTGCTGGCAGATGACCCACTAGAATTTATTGGAAAGGCTGGCGA
CAGTGGCGGAGGAATGTATGTCATCAACCTCCATAAGGCATTAGCATCCCTATCCACTGCAACTCTGGAG
TCTGTCATCCAAGAGAGATTCGGGTCTCGCTGTGCCCGGATATTCCGTCTGGTGTTGCAGAAGAAGCACC
TGGAGCAGAAGCAGGTGGAGGACTTTGCAATGATCCCTGCAAAGGAGGCAAAGGACATGCTGTATAAGAT
GCTCTCGGAGAACTTCATATCACTGCAGGAAATTCCTAAAACGCCAGACCACGCCCCGTCCAGGACCTTC
TATCTGTACACCGTGAATGTGCTGTCAGCCGCCAGGATGCTGTTGCACAGGTGCTACAAGAGCATAGCCA
ACTTGATAGAAAGGCGGCAATTTGAAACAAAAGAGAACAAGCGGCTACTAGAAAAGTCTCAGCGGGTGGA
AGCCATCATGGCATCAATGCAGGCCACGGGTGCAGAGGAGGTACAGCTGCAGGAGATCGAGGAGATGATC
ACAGCCCCCGAGCGGCAGCAGCTGGAGACACTGAAACGCAACGTTAACAAGTTGGATGCCAGTGAGATCC
AGGTAGATGAAACCATCTTCTTACTGGAATCATACATCGAGAGCACCATGAAGAGACAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR214037 representing NM_001012081
Red=Cloning site Green=Tags(s)

MTQAEIKLCSLLLQEHFGEIVEKIGVHLIRTGSQPLRVIAHDTKASLDQVKKALCVLIHHNLVIYHVHKR
GVVEYEAQCSRVLRMLRYPRYIYTTKTLYGDTGELIVEELLLNGKMTMSAVVKKVADRLTETMEDGKTMD
YAEVSNAFVRLADTHFVQRCPLVPDTDSSDPGPPPPAPNLVINEKDMYLVPKLSLIGKGKRRRSSDEDAA
GEPKAKKPRCTDNEEPTPDDGIYWQVNLDRFHQHFRDQAIVSAVANRMDQTSSEIVRTMLRMSEITTPSG
APFTQPLSSNEIFRSLPVGYNISKQVLDQYLTLLADDPLEFIGKAGDSGGGMYVINLHKALASLSTATLE
SVIQERFGSRCARIFRLVLQKKHLEQKQVEDFAMIPAKEAKDMLYKMLSENFISLQEIPKTPDHAPSRTF
YLYTVNVLSAARMLLHRCYKSIANLIERRQFETKENKRLLEKSQRVEAIMASMQATGAEEVQLQEIEEMI
TAPERQQLETLKRNVNKLDASEIQVDETIFLLESYIESTMKRQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001012081
ORF Size 1599 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001012081.1, NP_001012081.1
RefSeq Size 2394 bp
RefSeq ORF 1602 bp
Locus ID 310685
Cytogenetics 2q34
MW 60.5 kDa

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.