Fads1 (NM_053445) Rat Tagged ORF Clone

CAT#: RR214038

Fads1 (Myc-DDK-tagged ORF) - Rat fatty acid desaturase 1 (Fads1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fads1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214038 representing NM_053445
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCCCGACCCGGTGCAGACCCCTGACCCGGCCTCCGCCCAGCTCCGCCAAATGCGCTACTTTACTT
  GGGAGGAGGTGGCGCAGCGCTCCGGGAGGGAGAAGGAGCGATGGCTCGTGATCGACCGGAAGGTGTACAA
  CATCAGCGACTTCAGTCGCCGCCACCCGGGAGGCTCCCGGGTCATCAGCCACTACGCTGGTCAGGATGCC
  ACGGATCCTTTTGTGGCATTCCACATTAACAAGGGCCTTGTGAGAAAGTATATGAACTCTCTTCTGATTG
  GAGAGCTAGCTCCGGAGCAGCCCAGCTTTGAACCCACCAAGAATAAGGCGCTCACTGATGAATTCCGGGA
  GCTGCGGGCCACAGTGGAGCGAATGGGCCTCATGAAAGCCAACCATCTCTTCTTCCTGTTCTATCTGCTG
  CACATCCTGCTGCTGGACGTGGCCGCCTGGCTCACTCTTTGGATCTTTGGAACTTCCTTGGTGCCCTTCA
  CCCTCTGTGCAGTGCTGCTCAGTACAGTTCAGGCCCAGGCAGGTTGGCTACAGCATGACTTTGGGCACCT
  GTCCGTCTTCAGCACCTCAACATGGAATCACCTGGTACATCATTTTGTCATTGGCCACCTGAAGGGGGCC
  CCAGCCAGCTGGTGGAACCACATGCATTTCCAGCACCACGCCAAGCCCAACTGCTTCCGCAAAGACCCCG
  ATATCAACATGCATCCCCTCTTCTTCGCCCTGGGGAAGGTCCTTTCTGTGGAGCTTGGGAAAGAAAAGAA
  GAAGCACATGCCATACAACCATCAGCACAAGTACTTCTTCCTGATTGGACCCCCAGCCTTGCTGCCTCTC
  TACTTCCAGTGGTACATTTTCTATTTTGTTGTTCAGCGGAAGAAATGGGTGGACTTGGCCTGGATGCTCA
  GCTTCTATGTTCGTGTCTTCTTCACTTACATGCCGCTGCTGGGGCTGAAAGGCCTCCTATGTCTTTTCTT
  CATTGTCAGGTTCCTGGAGAGCAACTGGTTTGTGTGGGTGACGCAGATGAACCATATCCCCATGCACATT
  GATCATGACCGGAATGTGGACTGGGTCTCCACCCAGCTACAGGCAACCTGCAACGTTCACCAGTCAGCCT
  TCAACAACTGGTTCAGTGGCCACCTCAATTTCCAGATTGAACACCACCTCTTCCCTACGATGCCACGACA
  CAACTACCACAAGGTGGCACCCCTGGTACAATCTCTGTGCGCCAAGTACGGCATCAAGTATGAGTCCAAG
  CCCCTGCTCACGGCCTTCGCGGACATTGTTTACTCCCTGAAGGAGTCAGGACAGCTCTGGCTGGATGCTT
  ACCTTCACCAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214038 representing NM_053445
  Red=Cloning site Green=Tags(s)
  
  MAPDPVQTPDPASAQLRQMRYFTWEEVAQRSGREKERWLVIDRKVYNISDFSRRHPGGSRVISHYAGQDA
  TDPFVAFHINKGLVRKYMNSLLIGELAPEQPSFEPTKNKALTDEFRELRATVERMGLMKANHLFFLFYLL
  HILLLDVAAWLTLWIFGTSLVPFTLCAVLLSTVQAQAGWLQHDFGHLSVFSTSTWNHLVHHFVIGHLKGA
  PASWWNHMHFQHHAKPNCFRKDPDINMHPLFFALGKVLSVELGKEKKKHMPYNHQHKYFFLIGPPALLPL
  YFQWYIFYFVVQRKKWVDLAWMLSFYVRVFFTYMPLLGLKGLLCLFFIVRFLESNWFVWVTQMNHIPMHI
  DHDRNVDWVSTQLQATCNVHQSAFNNWFSGHLNFQIEHHLFPTMPRHNYHKVAPLVQSLCAKYGIKYESK
  PLLTAFADIVYSLKESGQLWLDAYLHQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053445
• ORF Size: 1341 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_053445.1, NM_053445.2, NP_445897.2
• RefSeq Size: 3380
• RefSeq ORF: 1344
• Locus ID: 84575
• MW: 52.5 kDa
• Gene Summary: catalyzes the Delta(5,6) desaturation of fatty acids of the n-6 and n-3 series [RGD, Feb 2006]