Tuba1c (NM_001011995) Rat Tagged ORF Clone

CAT#: RR214069

Tuba1c (Myc-DDK-tagged ORF) - Rat tubulin, alpha 1C (Tuba1c), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tuba1c
• Synonyms: Tuba6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214069 representing NM_001011995
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGTGAGTGCATCTCCATCCACGTTGGCCAGGCTGGTGTCCAGATCGGCAATGCCTGCTGGGAGCTCT
  ACTGCCTGGAACATGGCATCCAGCCTGATGGCCAGATGCCAAGTGACAAGACCATTGGGGGAGGAGATGA
  CTCCTTCAACACCTTCTTCAGTGAGACAGGAGCTGGCAAGCACGTGCCCCGGGCGGTGTTCGTAGACCTG
  GAACCCACAGTTATTGATGAAGTTCGAACTGGCACCTACCGCCAGCTCTTCCACCCAGAGCAGCTCATCA
  CAGGCAAGGAAGATGCTGCCAATAACTATGCCCGTGGCCACTACACCATTGGCAAGGAGATCATTGACCT
  TGTCTTGGACAGAATTCGCAAGCTGGCTGACCAGTGCACGGGTCTCCAGGGCTTCTTGGTTTTCCACAGC
  TTTGGTGGGGGAACTGGCTCTGGGTTCACCTCCCTGCTGATGGAGAGGCTCTCTGTCGACTACGGAAAGA
  AGTCCAAGCTGGAGTTCTCCATTTACCCAGCCCCCCAGGTTTCCACTGCTGTGGTTGAGCCCTACAATTC
  CATCCTCACCACCCACACCACCCTGGAGCACTCTGATTGTGCCTTCATGGTAGACAATGAGGCCATCTAT
  GACATCTGTCGTAGAAACCTCGACATTGAGCGCCCAACCTACACTAACCTTAACCGCCTTATTAGCCAGA
  TTGTGTCTTCCATCACTGCTTCCCTCAGATTTGATGGGGCCCTGAATGTTGATCTGACAGAATTCCAGAC
  CAACCTGGTGCCCTACCCTCGAATCCACTTCCCTCTGGCCACTTATGCCCCTGTCATCTCTGCTGAGAAA
  GCCTACCATGAGCAACTTACAGTAGCAGAGATCACCAATGCCTGCTTTGAGCCAGCCAACCAGATGGTGA
  AATGTGACCCTCGCCATGGTAAATACATGGCTTGCTGCCTGCTGTACCGTGGTGATGTGGTCCCCAAGGA
  TGTCAATGCTGCCATTGCCACCATCAAGACCAAGCGTACCATCCAGTTTGTGGACTGGTGCCCCACTGGC
  TTCAAGGTTGGCATTAATTACCAGCCTCCCACTGTGGTACCTGGTGGCGACCTGGCCAGGGTGCAGAGAG
  CTGTGTGTATGCTGAGCAACACCACAGCCATTGCTGAGGCCTGGGCTCGCCTGGATCACAAGTTTGATCT
  GATGTATGCCAAGCGTGCCTTTGTGCACTGGTATGTGGGTGAGGGCATGGAGGAGGGGGAGTTCTCTGAG
  GCCCGTGAGGATATGGCTGCCCTAGAGAAGGATTATGAGGAGGTTGGGGCAGATAGTGCTGAAGGAGACG
  ATGAGGGTGAAGAATAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214069 representing NM_001011995
  Red=Cloning site Green=Tags(s)
  
  MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDL
  EPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHS
  FGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIY
  DICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEK
  AYHEQLTVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTG
  FKVGINYQPPTVVPGGDLARVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
  AREDMAALEKDYEEVGADSAEGDDEGEEY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001011995
• ORF Size: 1347 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001011995.1, NP_001011995.1
• RefSeq Size: 1559 bp
• RefSeq ORF: 1350 bp
• Locus ID: 300218
• Cytogenetics: 7q36
• MW: 49.9 kDa
• Gene Summary: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [UniProtKB/Swiss-Prot Function]