Plk1 (NM_017100) Rat Tagged ORF Clone

CAT#: RR214157

Plk1 (Myc-DDK-tagged ORF) - Rat polo-like kinase 1 (Drosophila) (Plk1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Plk1
• Synonyms: Plk
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214157 representing NM_017100
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGCAGCGGCCAAAGCTGGAAAGCTGGCTCGAGCACCAGCCGACCTCGGGAAAGGTGGGGTTCCGG
  GAGATGCAGTTCCCGGTGCCCCAGTGGCCGCCCCGCTGGCGAAAGAAATTCCGGAGGTCCTAGTGGACCC
  ACGCAGCCGGCAGCAGTATGTACGGGGCCGCTTCCTGGGTAAAGGAGGCTTCGCCAAATGCTTCGAAATC
  TCAGACTCAGACACAAAAGAGGTGTTCCCAGGCAAGATCGTGCCTAAGTCTTTGCTGCTCAAGCCCCATC
  AGAAGGAGAAAATGTCTATGGAGACCTCCATTCACCGCAGCCTCGAACACCAGCACGTCGTAGGCTTCCA
  TGGCTTTTTTGAGGACAGCGACTTTGTGTTCGTGGTTTTGGAGCTCTGTCGCAGGAGGTCTCTCCTGGAG
  CTGCACAAAAGGAGGAAGGCGCTGACCGAGCCCGAGGCCCGATACTACCTGCGGCAGATAGTCCTGGGTT
  GCCAGTACCTGCACCGCAATCAAGTCATTCACAGGGACCTCAAACTGGGCAACCTCTTTCTGAATGAGGA
  TCTGGAGGTGAAAATAGGGGATTTTGGCCTGGCAACAAAAGTGGAATATGAAGGGGAACGGAAGAAGACC
  CTGTGTGGCACTCCTAACTACATAGCTCCCGAGGTGCTGAGCAAGAAGGGACACAGCTTTGAGGTGGACG
  TGTGGTCCATTGGGTGCATCATGTATACCTTGCTGGTGGGCAAGCCACCTTTTGAGACCTCATGCCTAAA
  AGAGACCTACCTCCGGATCAAGAAAAATGAATACAGTATTCCCAAGCACATCAACCCAGTGGCTGCCTCC
  CTCATCCAGAAAATGCTTCAGACAGACCCCGCTGCGCGCCCCACCATTCACGAGTTGCTCAATGACGAGT
  TCTTCACTTCCGGATACATCCCCGCCCGTCTGCCTATTACCTGCCTCACCATCCCACCAAGGTTTTCAAT
  AGCTCCCAGCAGCCTAGACCCCAGCAACAGGAAGCCTCTCACAGTCCTTAATAAAGGTGTGGAGAACCCC
  CTGCCTGACCGTCCCCGAGAGAAAGAGGAACCAGTGGTTCGGGAGACAAATGAGGCCATCGAGTGCCACC
  TTAGTGACTTGCTACAGCAGCTGACCAGTGTCAACGCCTCCAAGCCCTCAGAGCGAGGGCTGGTGCGGCA
  AGAGGAGGCCGAGGATCCTGCTTGCATCCCTATCTTCTGGGTCAGCAAATGGGTAGACTATTCGGACAAG
  TATGGCCTTGGTTATCAGCTATGTGACAACAGTGTGGGAGTGCTCTTTAATGACTCAACACGCCTCATCC
  TCTACAATGACGGGGACAGCCTGCAGTACATAGAGCGTGATGGCACGGAGTCCTACCTCACTGTGAGCTC
  CCACCCCAACTCCTTGATGAAGAAGATCACTCTCCTCAACTATTTCCGCAATTACATGAGTGAACACCTG
  CTGAAGGCGGGGGCCAACATCACGCCCCGGGAAGGCGACGAGCTGGCCCGGCTGCCCTACCTACGAACAT
  GGTTCCGCACACGCAGCGCCATCATCCTGCACCTCAGCAATGGCACAGTACAGATTAATTTCTTCCAGGA
  CCACACCAAACTTATCCGTGGCCCCCTGATGGCAGCAGTGACCTACATCAACGAGAAGAGGGACTTCCGG
  ACATACCGCCTGAGCCTTCTGGAGGAATATGGCTGCTGCAAGGAACTGGCTAGCCGCCTCCGCTATGCCC
  GCACCATGGTAGACAAGCTGCTGAGCTCGCGTTCCGCCTGCAACCGCCTCAAGGCCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214157 representing NM_017100
  Red=Cloning site Green=Tags(s)
  
  MNAAAKAGKLARAPADLGKGGVPGDAVPGAPVAAPLAKEIPEVLVDPRSRQQYVRGRFLGKGGFAKCFEI
  SDSDTKEVFPGKIVPKSLLLKPHQKEKMSMETSIHRSLEHQHVVGFHGFFEDSDFVFVVLELCRRRSLLE
  LHKRRKALTEPEARYYLRQIVLGCQYLHRNQVIHRDLKLGNLFLNEDLEVKIGDFGLATKVEYEGERKKT
  LCGTPNYIAPEVLSKKGHSFEVDVWSIGCIMYTLLVGKPPFETSCLKETYLRIKKNEYSIPKHINPVAAS
  LIQKMLQTDPAARPTIHELLNDEFFTSGYIPARLPITCLTIPPRFSIAPSSLDPSNRKPLTVLNKGVENP
  LPDRPREKEEPVVRETNEAIECHLSDLLQQLTSVNASKPSERGLVRQEEAEDPACIPIFWVSKWVDYSDK
  YGLGYQLCDNSVGVLFNDSTRLILYNDGDSLQYIERDGTESYLTVSSHPNSLMKKITLLNYFRNYMSEHL
  LKAGANITPREGDELARLPYLRTWFRTRSAIILHLSNGTVQINFFQDHTKLIRGPLMAAVTYINEKRDFR
  TYRLSLLEEYGCCKELASRLRYARTMVDKLLSSRSACNRLKAS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017100
• ORF Size: 1809 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_017100.1, NP_058796.1
• RefSeq Size: 2198
• RefSeq ORF: 1812
• Locus ID: 25515
• MW: 68.3 kDa
• Gene Summary: human homolog catalyzes the phosphorylation of a Golgi reassembly stacking protein (GRASP65); may play a role in Golgi apparatus fragmentation and reorganization during mitosis [RGD, Feb 2006]