Mapk3 (NM_017347) Rat Tagged ORF Clone

CAT#: RR214231

Mapk3 (Myc-DDK-tagged ORF) - Rat mitogen activated protein kinase 3 (Mapk3), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mapk3
• Synonyms: Erk-1; ERK1; ERT2; Esrk1; MAPK1; MNK1; p44; p44erk1; p44mapk; Prkm3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214231 representing NM_017347
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGCGGCGGCGGCTCCGGGGGGCGGGGGCGGGGAGCCCAGGGGAACTGCTGGGGTCGTCCCGG
  TGGTCCCCGGGGAGGTGGAGGTGGTGAAGGGGCAGCCATTCGACGTGGGCCCACGCTACACGCAGCTGCA
  GTACATCGGCGAGGGCGCGTACGGCATGGTCAGCTCAGCATATGACCACGTGCGCAAGACCAGAGTGGCT
  ATCAAGAAGATCAGCCCCTTCGAGCATCAAACCTACTGTCAGCGCACGCTGAGAGAAATCCAGATCTTGC
  TCGGATTCCGCCATGAGAATGTCATAGGCATCCGAGACATCCTCAGAGCACCCACCCTGGAAGCCATGAG
  AGATGTTTACATTGTTCAGGACCTCATGGAGACGGACCTGTACAAGCTGCTAAAGAGCCAGCAGCTGAGC
  AATGACCACATCTGCTACTTCCTCTACCAGATCCTCCGGGGCCTCAAGTACATACACTCGGCCAATGTGC
  TGCACCGGGACCTGAAGCCCTCCAATCTGCTTATCAACACCACCTGCGACCTTAAGATCTGTGATTTTGG
  CCTTGCCCGGATTGCTGACCCTGAGCACGACCACACTGGCTTTCTGACCGAGTATGTGGCCACACGCTGG
  TACCGAGCCCCAGAGATCATGCTTAACTCCAAGGGCTACACCAAATCCATTGACATCTGGTCTGTGGGCT
  GCATTCTGGCTGAGATGCTCTCCAACCGGCCTATCTTCCCCGGCAAGCACTACCTGGACCAGCTCAACCA
  CATTCTAGGTATACTGGGTTCCCCATCCCAAGAGGACCTAAATTGTATCATTAACATGAAGGCCCGAAAC
  TACCTACAGTCTCTGCCCTCTAAAACCAAGGTGGCTTGGGCCAAGCTTTTTCCCAAATCTGACTCCAAAG
  CTCTTGACCTGCTGGACCGGATGTTAACCTTTAACCCAAACAAGCGCATCACAGTAGAGGAAGCACTGGC
  TCACCCTTACCTGGAACAGTACTATGATCCGACAGATGAACCAGTGGCTGAGGAGCCATTCACCTTTGAC
  ATGGAGCTGGATGATCTCCCCAAGGAGCGGCTGAAGGAGCTGATCTTCCAAGAGACAGCCCGCTTCCAGC
  CAGGGGCACCAGAGGCCCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214231 representing NM_017347
  Red=Cloning site Green=Tags(s)
  
  MAAAAAAPGGGGGEPRGTAGVVPVVPGEVEVVKGQPFDVGPRYTQLQYIGEGAYGMVSSAYDHVRKTRVA
  IKKISPFEHQTYCQRTLREIQILLGFRHENVIGIRDILRAPTLEAMRDVYIVQDLMETDLYKLLKSQQLS
  NDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLINTTCDLKICDFGLARIADPEHDHTGFLTEYVATRW
  YRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINMKARN
  YLQSLPSKTKVAWAKLFPKSDSKALDLLDRMLTFNPNKRITVEEALAHPYLEQYYDPTDEPVAEEPFTFD
  MELDDLPKERLKELIFQETARFQPGAPEAP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017347
• ORF Size: 1140 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_017347.1, NM_017347.2, NP_059043.1
• RefSeq Size: 1875
• RefSeq ORF: 1143
• Locus ID: 50689
• MW: 43 kDa
• Gene Summary: kinase involved in intracellular signalling; component of Mapk signalling pathway [RGD, Feb 2006]