Pxn (NM_001012147) Rat Tagged ORF Clone

CAT#: RR214314

Pxn (Myc-DDK-tagged ORF) - Rat paxillin (Pxn), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pxn
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214314 representing NM_001012147
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGACCTCGACGCCCTGCTGGCAGACTTGGAGTCCACCACCTCCCACATCTCCAAACGGCCAGTGT
  TCTTGTCAGAGGAGCCCCCCTACTCCTACCCAACTGGAAACCACACTTACCAGGAGATTGCAGTGCCACC
  TCCGGTCCCACCGCCCCCATCCAGCGAGGCCCTCAATGGCACAGTCCTGGACCCCTTAGACCAATGGCAG
  CCTAGTGGTTCCAGATACGCTCACCAGCAGCCTCCATCCCCATCGCCCATATACAGCTCCAGCACCAAAA
  ATTCCAGTGCCTCCAACCCTCAGGACAGTGTCGGCTCCCTTTGTTCCCGAGCAGGCGAGGAGGAACACGT
  CTACAGCTTCCCCAACAAGCAGAAGTCAGCAGAGCCGTCACCCACCGTGATGAGCTCCTCCCTGGGCAGC
  AACCTCTCTGAGCTGGACCGGCTGTTACTGGAGCTGAACGCAGTGCAGCGTAGTCCTTCCGGCTTCTCTG
  CAGGTATGGTGTCAGTCCAGGCCTCTCGTGAGCCTTTGGGTTCCTGGGGGACGGAGGGACGAGCAATCAT
  TTTGTCCCCTTTTTTCCAAGATGAAGCAGAATCGAGCCCCCCATTGCCCGGTGCCCTGAGTCCCCTTTAT
  GGCGTCCCGGAGAGTAACAATCTCCTGGGGGGCAAAGCAGGGCCGCTCATGAAGGAGAAGCCAAAGCGAA
  ACGGAGGCCGGGGGCTGGAGGATGTGCGGCCCAGTGTGGAGAGCCTCTTGGATGAGCTGGAGAACTCTGT
  GCCCAGCCCCGTCCCGGCCATCACTGTGAACCAGGGAGAGATGAGCAGTCCACAGCGAGTCACCTCCAGC
  CAGCAGCAGACCAGAATCTCAGCCTCCTCTGCCACCAGGGAGCTGGACGAGCTCATGGCTTCATTGTCAG
  ATTTCAAGTTCATGGCCCAGGGGAAAACCGGGAGCAGCTCGCCTCCTGGGGGCCTCTCAAAACCTGGGAG
  CCAGCTTGACAGCATGTTGGGGAGTCTGCAGTCCGACCTGAACAAGCTAGGGGTCGCCACCGTTGCCAAA
  GGGGTTTGTGGAGCCTGCAAGAAGCCCATTGCTGGGCAGGTGGTGACTGCCATGGGGAAGACATGGCACC
  CCGAGCACTTTGTGTGCACCCACTGCCAGGAAGAGATTGGATCCCGGAACTTCTTCGAGCGGGACGGACA
  ACCCTACTGTGAAAAGGACTACCACAGCCTCTTCTCCCCACGCTGCTACTACTGCAACGGGCCCATCTTG
  GATAAAGTGGTGACAGCGCTGGACCGCACGTGGCACCCGGAACACTTCTTCTGTGCCCAGTGTGGAGCCT
  TCTTTGGTCCAGAAGGGTTCCACGAGAAAGATGGCAAAGCCTACTGTCGGAAGGATTACTTCGACATGTT
  CGCGCCCAAGTGTGGCGGCTGTGCCCGTGCCATCCTGGAGAACTACATCTCGGCCCTCAACACCCTCTGG
  CACCCTGAATGCTTTGTGTGCAGGGAGTGCTTCACACCCTTCGTCAATGGCAGCTTCTTCGAGCACGACG
  GGCAGCCATATTGTGAGGTGCACTACCACGAGCGTCGCGGCTCACTGTGCTCCGGCTGCCAGAAGCCCAT
  CACCGGCCGCTGCATCACCGCCATGGCCAAGAAGTTCCACCCCGAGCATTTCGTGTGCGCCTTTTGCCTC
  AAGCAGCTCAACAAGGGCACTTTCAAGGAGCAGAACGACAAGCCCTACTGTCAGAGCTGCTTCCTGAAGC
  TCTTCTGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214314 representing NM_001012147
  Red=Cloning site Green=Tags(s)
  
  MDDLDALLADLESTTSHISKRPVFLSEEPPYSYPTGNHTYQEIAVPPPVPPPPSSEALNGTVLDPLDQWQ
  PSGSRYAHQQPPSPSPIYSSSTKNSSASNPQDSVGSLCSRAGEEEHVYSFPNKQKSAEPSPTVMSSSLGS
  NLSELDRLLLELNAVQRSPSGFSAGMVSVQASREPLGSWGTEGRAIILSPFFQDEAESSPPLPGALSPLY
  GVPESNNLLGGKAGPLMKEKPKRNGGRGLEDVRPSVESLLDELENSVPSPVPAITVNQGEMSSPQRVTSS
  QQQTRISASSATRELDELMASLSDFKFMAQGKTGSSSPPGGLSKPGSQLDSMLGSLQSDLNKLGVATVAK
  GVCGACKKPIAGQVVTAMGKTWHPEHFVCTHCQEEIGSRNFFERDGQPYCEKDYHSLFSPRCYYCNGPIL
  DKVVTALDRTWHPEHFFCAQCGAFFGPEGFHEKDGKAYCRKDYFDMFAPKCGGCARAILENYISALNTLW
  HPECFVCRECFTPFVNGSFFEHDGQPYCEVHYHERRGSLCSGCQKPITGRCITAMAKKFHPEHFVCAFCL
  KQLNKGTFKEQNDKPYCQSCFLKLFC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001012147
• ORF Size: 1758 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012147.1, NP_001012147.1
• RefSeq Size: 3737 bp
• RefSeq ORF: 1761 bp
• Locus ID: 360820
• Cytogenetics: 12q16
• MW: 64.5 kDa