Gne (NM_053765) Rat Tagged ORF Clone

CAT#: RR214376

Gne (Myc-DDK-tagged ORF) - Rat glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (Gne), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gne
• Synonyms: Uae1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214376 representing NM_053765
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAAGAACGGGAATAACCGGAAGCTTCGGGTTTGCGTTGCCACCTGCAACCGAGCCGATTACTCCA
  AACTGGCCCCCATCATGTTCGGCATTAAGACGGAGCCTGCGTTCTTCGAGCTCGACGTGGTGGTGCTGGG
  CTCTCACCTGATCGACGACTACGGAAACACATACCGCATGATTGAGCAGGACGACTTTGACATCAACACC
  AGGCTACACACGATTGTTAGAGGGGAAGACGAAGCAGCCATGGTAGAGTCAGTGGGCCTAGCGCTAGTGA
  AGCTACCGGATGTCCTCAACCGCCTGAAGCCTGACATCATGATTGTTCACGGAGACCGATTTGACGCCCT
  CGCTCTGGCTACATCTGCTGCCCTGATGAACATCCGCATCCTTCACATTGAAGGAGGAGAGGTCAGCGGG
  ACTATTGATGACTCTATCAGACACGCCATAACAAAACTGGCTCACTACCACGTGTGCTGCACCAGGAGTG
  CAGAGCAACACCTGATCTCCATGTGTGAGGACCACGACCGCATCCTTTTGGCTGGCTGCCCTTCCTATGA
  CAAACTGCTCTCAGCCAAGAATAAAGACTATATGAGCATCATTCGGATGTGGCTAGGTGATGATGTAAAA
  TGTAAAGATTACATTGTTGCCCTGCAACACCCGGTGACCACCGACATTAAGCATTCCATAAAGATGTTCG
  AACTGACACTGGATGCTCTTATCTCATTTAACAAGAGGACCCTAGTTCTGTTTCCAAATATCGATGCAGG
  CAGCAAGGAGATGGTTCGAGTGATGCGGAAGAAGGGCATCGAGCATCACCCCAATTTCCGCGCAGTCAAG
  CACGTCCCGTTTGACCAGTTCATTCAGCTGGTCGCCCACGCTGGCTGCATGATTGGGAATAGCAGCTGTG
  GAGTGCGTGAGGTTGGCGCCTTTGGAACCCCTGTGATCAACCTGGGCACGCGGCAGATAGGAAGAGAAAC
  GGGGGAGAATGTTCTTCATGTCCGGGATGCTGACACCCAAGACAAAATATTACAAGCACTACACCTCCAG
  TTCGGTAAACAGTACCCTTGCTCAAAGATATATGGGGATGGAAATGCTGTTCCAAGGATTTTAAAGTTTC
  TCAAATCCATCGACCTTCAAGAGCCACTACAGAAGAAATTCTGCTTCCCTCCCGTGAAGGAGAACATCTC
  TCAGGATATTGACCATATCCTCGAAACTCTGAGTGCCTTGGCTGTTGATCTCGGGGGGACGAATCTGAGA
  GTGGCGATAGTTAGCATGAAGGGTGAAATAGTTAAGAAGTACACCCAGTTCAATCCTAAAACCTATGAGG
  AAAGGATTAGTCTAATCCTGCAGATGTGTGTGGAAGCGGCAGCAGAAGCCGTGAAGCTCAATTGCAGAAT
  TCTGGGAGTAGGCATCTCCACAGGTGGCCGTGTGAATCCCCAGGAAGGAGTTGTGCTGCACTCGACCAAG
  CTGATACAGGAGTGGAACTCTGTGGACCTCAGGACACCACTCTCCGACACCCTGCATCTCCCCGTGTGGG
  TGGACAACGACGGCAACTGCGCTGCCATGGCGGAGAGGAAGTTTGGCCAAGGAAAAGGACAGGAGAACTT
  TGTGACGCTCATCACAGGGACAGGGATCGGTGGGGGAATCATCCACCAGCACGAGCTGATCCACGGCAGC
  TCCTTCTGTGCGGCAGAGCTTGGCCACCTCGTGGTGTCTCTGGATGGTCCTGACTGCTCCTGTGGAAGCC
  ATGGGTGCATTGAAGCCTACGCCTCTGGAATGGCCTTGCAGAGGGAAGCAAAGAAGCTCCACGACGAGGA
  CCTGCTCTTGGTGGAAGGGATGTCAGTGCCAAAAGACGAAGCTGTGGGCGCCCTCCATCTCATCCAAGCC
  GCCAAGCTGGGCAACGTGAAGGCCCAGAGCATCTTACGGACAGCTGGAACTGCTTTGGGACTCGGAGTTG
  TGAATATCCTCCACACTATGAATCCTTCCCTGGTGATCCTGTCTGGAGTCCTGGCTAGTCACTACATCCA
  CATTGTGAGGGACGTCATCCGCCAGCAAGCCCTGTCCTCCGTGCAGGATGTGGATGTAGTGGTTTCAGAC
  TTGGTTGACCCGGCCCTGCTTGGTGCGGCCAGCATGGTTCTGGACTACACGACCCGCAGGATCCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214376 representing NM_053765
  Red=Cloning site Green=Tags(s)
  
  MEKNGNNRKLRVCVATCNRADYSKLAPIMFGIKTEPAFFELDVVVLGSHLIDDYGNTYRMIEQDDFDINT
  RLHTIVRGEDEAAMVESVGLALVKLPDVLNRLKPDIMIVHGDRFDALALATSAALMNIRILHIEGGEVSG
  TIDDSIRHAITKLAHYHVCCTRSAEQHLISMCEDHDRILLAGCPSYDKLLSAKNKDYMSIIRMWLGDDVK
  CKDYIVALQHPVTTDIKHSIKMFELTLDALISFNKRTLVLFPNIDAGSKEMVRVMRKKGIEHHPNFRAVK
  HVPFDQFIQLVAHAGCMIGNSSCGVREVGAFGTPVINLGTRQIGRETGENVLHVRDADTQDKILQALHLQ
  FGKQYPCSKIYGDGNAVPRILKFLKSIDLQEPLQKKFCFPPVKENISQDIDHILETLSALAVDLGGTNLR
  VAIVSMKGEIVKKYTQFNPKTYEERISLILQMCVEAAAEAVKLNCRILGVGISTGGRVNPQEGVVLHSTK
  LIQEWNSVDLRTPLSDTLHLPVWVDNDGNCAAMAERKFGQGKGQENFVTLITGTGIGGGIIHQHELIHGS
  SFCAAELGHLVVSLDGPDCSCGSHGCIEAYASGMALQREAKKLHDEDLLLVEGMSVPKDEAVGALHLIQA
  AKLGNVKAQSILRTAGTALGLGVVNILHTMNPSLVILSGVLASHYIHIVRDVIRQQALSSVQDVDVVVSD
  LVDPALLGAASMVLDYTTRRIH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053765
• ORF Size: 2166 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_053765.1, NM_053765.2, NP_446217.1
• RefSeq Size: 2843
• RefSeq ORF: 2169
• Locus ID: 114711
• MW: 79.2 kDa
• Gene Summary: bifunctional enzyme, catalyzes the first two steps of biosynthesis of Neu5Ac (UDP-GlcNAc 2-epimerase and ManNAc kinase), precursor of sialic acids [RGD, Feb 2006]