Igfbp4 (NM_001004274) Rat Tagged ORF Clone

CAT#: RR214384

Igfbp4 (Myc-DDK-tagged ORF) - Rat insulin-like growth factor binding protein 4 (Igfbp4), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Igfbp4
• Synonyms: IBP4; IGF-BP4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214384 representing NM_001004274
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCCCTTCGGCCTCGTGGCCGCCCTGCTGCTGGCCGCAGGGCCTCGGCCGAGCCTGGGCGACGAAG
  CCATCCACTGCCCTCCTTGCTCCGAGGAGAAGCTGGCGCGCTGCCGCCCCCCCGTGGGTTGTGAGGAGCT
  GGTGCGGGAGCCTGGCTGCGGTTGTTGTGCCACTTGCGCCCTGGGCTTGGGGATGCCTTGCGGGGTGTAC
  ACCCCACGCTGTGGCTCAGGCATGCGCTGCTACCCGCCCCGGGGGGTGGAGAAGCCCCTGCGCACATTGA
  TGCACGGGCAAGGTGTATGCACGGAGCTGTCGGAAATCGAAGCCATCCAGGAAAGCCTGCAGACCTCTGA
  CAAGGATGAGAGTGAGCATCCCAACAACAGCTTCAACCCCTGCAGCGCCCACGATCACAGGTGCCTGCAG
  AAGCATATGGCCAAAGTGAGAGATCGGAGCAAGATGAAGGTTGTGGGGACACCTCGGGAGGAACCCCGTC
  CTGTGCCCCAGGGTTCTTGCCAGAGCGAGCTGCACCGGGCCCTGGAACGACTGGCCGCCTCGCAGAGCCG
  TACCCACGAAGACCTCTTCATCATCCCCATCCCCAACTGTGACCGCAACGGCAACTTCCACCCCAAACAG
  TGTCATCCTGCTCTGGATGGACAGCGTGGCAAATGCTGGTGTGTGGATCGGAAGACAGGGGTGAAGCTTC
  CAGGGGGCTTGGAACCCAAGGGGGAGCTGGACTGCCACCAACTGGCTGACAGCCTCCAAGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214384 representing NM_001004274
  Red=Cloning site Green=Tags(s)
  
  MLPFGLVAALLLAAGPRPSLGDEAIHCPPCSEEKLARCRPPVGCEELVREPGCGCCATCALGLGMPCGVY
  TPRCGSGMRCYPPRGVEKPLRTLMHGQGVCTELSEIEAIQESLQTSDKDESEHPNNSFNPCSAHDHRCLQ
  KHMAKVRDRSKMKVVGTPREEPRPVPQGSCQSELHRALERLAASQSRTHEDLFIIPIPNCDRNGNFHPKQ
  CHPALDGQRGKCWCVDRKTGVKLPGGLEPKGELDCHQLADSLQE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001004274
• ORF Size: 762 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004274.1, NM_001004274.2, NP_001004274.1
• RefSeq Size: 2194 bp
• RefSeq ORF: 765 bp
• Locus ID: 360622
• Cytogenetics: 10q31
• MW: 27.7 kDa
• Gene Summary: may be involved in prolonging the half-life of the insulin growth factor [RGD, Feb 2006]