Slc12a2 (NM_031798) Rat Tagged ORF Clone

CAT#: RR214390

Slc12a2 (Myc-DDK-tagged ORF) - Rat solute carrier family 12 (sodium/potassium/chloride transporters), member 2 (Slc12a2), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Slc12a2
• Synonyms: Bsc2; Nkcc1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214390 representing NM_031798
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCCGGGCCCGCGGCGCCCTCGTCTGGCGCCCCGCGGCCCGCCCGGGATGGGGACGCGCCGCTGA
  CTGCCGCCGGGGTGGACCTGCCCGGCACGGCCGTACTCTCCGGTCGAGAGGATGCTACCGCTGGGAGCCA
  GGCCGGCGGCGGGGTGCGCGGAGAGGGAACCCCGGCCGCTGGCGATGGGCTGGGCAAACCTCTGGGGCCC
  ACCCCCAGCCAGAGCCGCTTCCAGGTGGACCCGGTTTCCGAGAACGCCGGGCGGGCCGCGGCTGCTGCAG
  CTGCAGCGGCGGCTGCGGCGGCTGCCGGGGCAGCGGGCAAGGAGACCCCCGCGGCTGGGAAAGCCGGCAC
  TGAGAGCGGCGTGGCCAAGGGCAGCGAGGAAGCCAAGGGCCGCTTCCGCGTGAACTTCGTGGACCCGGCT
  GCCTCCTCGTCGGCGGACGACAGCCTGTCGGATGCGGCGGGAGTCGGTGGCGACGGGCCCAACGTGAGCT
  CCCAGAACGGCGGGGACACGGTGCTGAGCGAGGGCAGCAGCCTGCACTCGGGTGGTGGCAGTGGACACCA
  CCAGCAGTACTACTATGACACCCACACCAACACTTACTACCTGCGCACCTTCGGCCACAACACCATGGAC
  GCGGTGCCCAGGATCGACCACTACCGGCACACGGCCGCGCAGCTGGGCGAGAAGCTGCTCCGGCCCAGCC
  TGGGAGAGTTCCACGATGAGCTGGAAAAGGAACCTTTTGAGGATGGTTTTGCGAATGGAGAAGAAAGTAC
  TCCAACCAGAGATGCTGTGGTCACATACACTGCCGAAAGTAAAGGAGTTGTAAAGTTTGGCTGGATCAAG
  GGTGTTTTAGTACGATGTATGTTGAATATTTGGGGTGTGATGCTCTTTATTCGATTGTCATGGATTGTGG
  GTCAGGCTGGAATAGGTCTCTCCGTTCTTGTAATAGCAATGGCCACTGTTGTGACAACCATCACAGGATT
  GTCTACTTCAGCAATAGCTACCAATGGATTCGTGAGAGGAGGAGGAGCATACTATTTAATCTCTAGAAGT
  CTAGGGCCAGAATTTGGCGGTGCGATTGGCTTGATCTTCGCTTTTCCCAATGCAGTGGCAGTTGCTATGT
  ATGTTGTTGGATTCGCAGAGACTGTAGTGGAGCTGCTTAAGGAACATTCCATACTTATGATAGATGAAAT
  CAATGACATCCGAATTATTGGAGCCATTACAGTGGTGGTTCTTCTGGGCATCTCAGTGGCCGGAATGGAG
  TGGGAAGCAAAGGCTCAGATCGTCCTTCTGGTGATTCTTCTTCTGGCCATCGCTGACTTCGTCATAGGAA
  CATTTATCTCACTGGACAGCAAGAAGCCCAAGGGTTTCTTTGGCTATAAATCTGAAATATTTAGTGAGAA
  CTTTGGACCGGATTTTCGAGAAGAAGAGACTTTCTTTTCTGTATTCGCCATCTTTTTTCCTGCTGCAACT
  GGTATTCTAGCTGGGGCGAACATCTCGGGTGACCTTGCAGATCCTCAGTCAGCCATACCCAAAGGAACGC
  TCTTAGCCATTTTGATTACGACAGTGGTTTACATAGGAATCGCAGTGTCTGTAGGTTCTTGTGTTGTCCG
  GGATGCCACTGGGAACGTTAATGACACCATTACAACAGAGCTAACTAACTGTACTTCTGCAGCCTGCAAA
  TTAAACTTTGACTTTTCCTACTGTGAAAGCAACACCTGTTCTTATGGGCTAATGAACAACTTTCAGGTGA
  TGAGCATGGTGTCAGGATTTGCACCCCTGATCTCTGCGGGTATCTTTTCAGCCACACTCTCATCAGCACT
  GGCATCTCTTGTGAGTGCTCCCAAAATATTTCAGGCTCTATGTAAGGACAATATCTACCCAGCTTTCCAG
  ATGTTTGCGAAAGGTTATGGAAAAAATAATGAACCTCTTCGTGGTTACATCTTAACATTCTTAATTGCAC
  TTGGATTCATCTTAATTGCTGAATTGAATGTCATTGCTCCAATTATCTCTAATTTCTTTCTCGCATCATA
  CGCCTTAATCAACTTTTCTGTATTCCATGCGTCACTTGCAAAGTCTCCAGGGTGGCGTCCTGCGTTCAAG
  TACTACAACATGTGGATCTCCCTTACTGGAGCGATTCTCTGCTGCATAGTGATGTTCGTCATTAACTGGT
  GGGCTGCACTGCTCACATATGTGATAGTTCTTGGACTGTACATTTATGTCACTTACAAAAAGCCAGATGT
  GAACTGGGGATCCTCCACACAAGCGCTGACTTACCTCAGCGCACTGCAGCATTCAATCCGTCTTTCCGGG
  GTGGAGGACCATGTGAAAAACTTCAGGCCACAGTGTCTTGTAATGACAGGTTCTCCAAACTCACGGCCTG
  CTTTACTGCATCTTGTTCATGACTTCACGAAAAATGTTGGTTTAATGATCTGTGGCCATGTGCATATGGG
  TCCCCGAAGACAAGCTATGAAAGAGATGTCCATTGATCAAGCCAAATATCAGCGATGGCTCATTAAAAAC
  AAAATGAAGGCTTTCTACGCTCCGGTTCACGCAGATGACTTGCGGGAAGGTGCACAGTACCTGATGCAGG
  CTGCTGGACTTGGTCGTATGAAACCAAACACACTTGTCCTCGGATTTAAGAAAGATTGGTTACAAGCAGA
  TATGAGGGATGTGGATATGTATATAAACTTATTTCATGATGCTTTTGACATACAGTATGGAGTTGTGGTC
  ATTCGCCTAAAGGAAGGACTGGATATATCACACCTCCAAGGACAAGAAGAATTATTGTCATCACAAGAAA
  AGTCACCTGGTACCAAGGATGTGGTAGTAAATGTGGACTACAGTAAGAAGTCAGATCAAGATGCTTTCAA
  AGCATCTGGTGAAAAACCTATTACACAGAAAGATGAGGAAGAGGATGGCAAGACTTCAACTCAGCCACTG
  TTGAAAAAAGAATCCAAGGGCCCCGTGGCACCTTTAAATGTAGCTGACCAAAAGCTTCTTGAAGCTAGCA
  CACAGTTTCAGAAAAAACAAGGGAAGAATACTATAGATGTCTGGTGGCTTTTTGATGATGGAGGTTTGAC
  CTTGTTGATACCCTATCTTCTGACAACCAAGAAAAAGTGGAAAGATTGTAAGATCCGAGTATTCATTGGT
  GGAAAGATAAACAGAATAGACCATGACCGGAGAGCGATGGCTACTTTACTCAGTAAATTCCGAATAGACT
  TCTCCGATATCATGGTCCTAGGAGACATCAACACCAAACCAAAAAAGGAAAACATCGTCGCTTTTGATGA
  CATGATTGAGCCATACAGACTTCACGAGGATGATAAAGAGCAGGATATTGCAGACAAAATGAAAGAGGAT
  GAGCCCTGGCGCATCACAGACAACGAGCTGGAGCTCTACAAGACCAAGACATATCGGCAGATCAGGTTAA
  ATGAATTATTAAAGGAGCATTCAAGCACAGCTAATATTATTGTCATGAGTCTTCCAGTTGCCCGGAAGGG
  TGCTGTGTCCAGCGCGCTGTACATGGCTTGGTTAGAAGCTCTGTCTAAGGACCTGCCACCAATCCTGCTG
  GTTCGTGGGAATCATCAGAGCGTCCTTACCTTCTACTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214390 representing NM_031798
  Red=Cloning site Green=Tags(s)
  
  MEPGPAAPSSGAPRPARDGDAPLTAAGVDLPGTAVLSGREDATAGSQAGGGVRGEGTPAAGDGLGKPLGP
  TPSQSRFQVDPVSENAGRAAAAAAAAAAAAAAGAAGKETPAAGKAGTESGVAKGSEEAKGRFRVNFVDPA
  ASSSADDSLSDAAGVGGDGPNVSSQNGGDTVLSEGSSLHSGGGSGHHQQYYYDTHTNTYYLRTFGHNTMD
  AVPRIDHYRHTAAQLGEKLLRPSLGEFHDELEKEPFEDGFANGEESTPTRDAVVTYTAESKGVVKFGWIK
  GVLVRCMLNIWGVMLFIRLSWIVGQAGIGLSVLVIAMATVVTTITGLSTSAIATNGFVRGGGAYYLISRS
  LGPEFGGAIGLIFAFPNAVAVAMYVVGFAETVVELLKEHSILMIDEINDIRIIGAITVVVLLGISVAGME
  WEAKAQIVLLVILLLAIADFVIGTFISLDSKKPKGFFGYKSEIFSENFGPDFREEETFFSVFAIFFPAAT
  GILAGANISGDLADPQSAIPKGTLLAILITTVVYIGIAVSVGSCVVRDATGNVNDTITTELTNCTSAACK
  LNFDFSYCESNTCSYGLMNNFQVMSMVSGFAPLISAGIFSATLSSALASLVSAPKIFQALCKDNIYPAFQ
  MFAKGYGKNNEPLRGYILTFLIALGFILIAELNVIAPIISNFFLASYALINFSVFHASLAKSPGWRPAFK
  YYNMWISLTGAILCCIVMFVINWWAALLTYVIVLGLYIYVTYKKPDVNWGSSTQALTYLSALQHSIRLSG
  VEDHVKNFRPQCLVMTGSPNSRPALLHLVHDFTKNVGLMICGHVHMGPRRQAMKEMSIDQAKYQRWLIKN
  KMKAFYAPVHADDLREGAQYLMQAAGLGRMKPNTLVLGFKKDWLQADMRDVDMYINLFHDAFDIQYGVVV
  IRLKEGLDISHLQGQEELLSSQEKSPGTKDVVVNVDYSKKSDQDAFKASGEKPITQKDEEEDGKTSTQPL
  LKKESKGPVAPLNVADQKLLEASTQFQKKQGKNTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRVFIG
  GKINRIDHDRRAMATLLSKFRIDFSDIMVLGDINTKPKKENIVAFDDMIEPYRLHEDDKEQDIADKMKED
  EPWRITDNELELYKTKTYRQIRLNELLKEHSSTANIIVMSLPVARKGAVSSALYMAWLEALSKDLPPILL
  VRGNHQSVLTFYS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031798
• ORF Size: 3609 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_031798.1, NP_113986.1
• RefSeq Size: 6402
• RefSeq ORF: 3612
• Locus ID: 83629
• MW: 130.4 kDa
• Gene Summary: Na/K/2Cl cotransporter involved in maintainence of intracellular Cl(-) levels [RGD, Feb 2006]