Gls2 (NM_138904) Rat Tagged ORF Clone

CAT#: RR214441

Gls2 (Myc-DDK-tagged ORF) - Rat glutaminase 2 (liver, mitochondrial) (Gls2), nuclear gene encoding mitochondrial protein, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gls2
• Synonyms: Ga
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214441 representing NM_138904
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGCCTCGACTTGGTGACCTGCTTTTCTACACCATTGCAGAGGGGCAGGAACGCATCCCCATCCATA
  AGTTCACCACGGCCCTGAAGGCCACTGGACTGCAGACGTCAGACCCACGGCTCCAGGACTGCATGAGCAA
  GATGCAGCGCATGGTCCAAGAGTCCAGCAGTGGTGGCCTCTTGGACCGAGAACTCTTCCAAAAGTGTGTG
  AGCAGCAACATTGTGCTCCTGACTCAGGCATTCCGGAAGAAGTTTGTCATTCCTGACTTTGAGGAGTTCA
  CAGGCCATGTGGATCGTATATTTGAGGATGCCAAAGAGCTCACTGGAGGCAAAGTGGCAGCCTACATCCC
  TCACCTGGCCAAGTCAAACCCAGATCTTTGGGGCGTCTCCCTGTGCACTGTGGATGGGCAGCGACACTCT
  GTGGGCCACACGAAGATCCCTTTCTGCCTACAGTCCTGTGTCAAGCCACTCACTTACGCCATCTCCGTGA
  GCACCTTAGGCACCGACTACGTGCACAAGTTCGTGGGCAAGGAACCCAGTGGTCTGCGCTATAACAAACT
  CTCCCTCAACGAGGAAGGAATCCCTCATAACCCCATGGTCAATGCTGGTGCTATTGTGGTCAGCTCCCTG
  ATCAAGATGGACTGTAACAAAGCAGAGAAGTTTGACTTTGTGTTACAGTATCTGAACAAGATGGCTGGGA
  ATGAATTCATGGGGTTCAGCAATGCCACATTCCAGTCAGAGAAGGAGACTGGGGATCGGAATTACGCCAT
  TGGCTATTACCTCAAGGAGAAGAAGTGCTTCCCTAAGGGAGTGGACATGATGGCTGCCCTTGATCTCTAT
  TTCCAGCTGTGCTCTGTGGAGGTTACCTGTGAGTCAGGCAGTGTCATGGCGGCCACTCTTGCCAATGGCG
  GCATCTGCCCCATCACAGGGGAGAGCGTGCTGAGTGCGGAAGCCGTGCGCAACACCCTCAGCCTCATGCA
  CTCCTGTGGCATGTATGACTTCTCGGGCCAGTTTGCCTTCCACGTGGGCCTGCCAGCCAAGTCAGCTGTG
  TCGGGAGCCATCCTCCTGGTTGTACCCAATGTCATGGGGATGATGTGTCTGTCACCCCCGTTAGACAAGC
  TGGGGAACAGCCACAGGGGCATCAGCTTCTGCCAGAAGTTGGTGTCTCTGTTTAACTTCCACAACTACGA
  CAACCTGCGGCACTGTGCTCGGAAGTTAGACCCACGGAGGGAAGGGGGGGAAGTTCGGAACAAGACCGTG
  GTGAACCTGTTATTTGCTGCATATAGTGGAGATGTCTCAGCTCTTCGAAGGTTTGCCTTGTCTGCCGTGG
  ATATGGAGCAGAAGGACTATGATTCCCGCACAGCCCTACATGTGGCGGCAGCGGAAGGACACATTGACGT
  TGTCAAGTTTCTGATCGAGGCTTGCAAAGTGAATCCTTTTGTCAAGGACAGGTGGGGCAACATTCCCCTG
  GATGATGCCGTGCAGTTCAATCACCTGGAGGTGGTCAAACTGCTTCAGGATTACCATGACTCCTACATGC
  TGTCTGAGACTCAAGCTGAGGTAGCAGCTGAGACTCTGTCAAAAGAGAACTTAGAGAGCATGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214441 representing NM_138904
  Red=Cloning site Green=Tags(s)
  
  MLPRLGDLLFYTIAEGQERIPIHKFTTALKATGLQTSDPRLQDCMSKMQRMVQESSSGGLLDRELFQKCV
  SSNIVLLTQAFRKKFVIPDFEEFTGHVDRIFEDAKELTGGKVAAYIPHLAKSNPDLWGVSLCTVDGQRHS
  VGHTKIPFCLQSCVKPLTYAISVSTLGTDYVHKFVGKEPSGLRYNKLSLNEEGIPHNPMVNAGAIVVSSL
  IKMDCNKAEKFDFVLQYLNKMAGNEFMGFSNATFQSEKETGDRNYAIGYYLKEKKCFPKGVDMMAALDLY
  FQLCSVEVTCESGSVMAATLANGGICPITGESVLSAEAVRNTLSLMHSCGMYDFSGQFAFHVGLPAKSAV
  SGAILLVVPNVMGMMCLSPPLDKLGNSHRGISFCQKLVSLFNFHNYDNLRHCARKLDPRREGGEVRNKTV
  VNLLFAAYSGDVSALRRFALSAVDMEQKDYDSRTALHVAAAEGHIDVVKFLIEACKVNPFVKDRWGNIPL
  DDAVQFNHLEVVKLLQDYHDSYMLSETQAEVAAETLSKENLESMV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138904
• ORF Size: 1605 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_138904.1, NM_138904.2, NP_620259.2
• RefSeq Size: 2247
• RefSeq ORF: 1608
• Locus ID: 192268
• MW: 59.2 kDa
• Gene Summary: first enzyme of glutamine catabolism in liver [RGD, Feb 2006]