Prmt7 (NM_001014153) Rat Tagged ORF Clone

CAT#: RR214544

Prmt7 (Myc-DDK-tagged ORF) - Rat protein arginine methyltransferase 7 (Prmt7), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Prmt7
• Synonyms: RGD1304869
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214544 representing NM_001014153
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGTCTTCTGTGGCCGTGCCAATCCAACCACGGGATCCCTGGAATGGCTGGAGGAGGATGAACACT
  ATGATTACCACCAAGAAATTGCCAGGTCATCCTATGCCGATATGCTACACGACAAAGACAGAAATATAAA
  ATATTACCAGGGTATCCGGGCAGCTGTGAGCAGAGTGAAAGACAAAGGACAGAAGGCCTTGGTTCTTGAC
  ATTGGCACTGGCACAGGCCTCTTATCAATGATGGCAGTTACCGCAGGGGCTGACTTCTGCTATGCTGTTG
  AGGTTTTTAAGCCAATGGCTGAGGCTGCTGTGAAGATTGTGGAGAAAAATGGCTTCAGTGATAAGATTAA
  AGTAATTAACAAGCATTCCACTGAGGTGACGGTTGGACCAGATGGTGACTTGCCATGCCGTGCTAACATT
  CTGGTTACGGAGCTGTTTGACACAGAGCTGATTGGGGAGGGAGCACTGCCCTCTTATGAGCACGCACACA
  AGCATCTTGTACAGGAAGACTGTGAAGCAGTGCCTCACAGGGCGACTGTCTATGCCCAGTTGGTGGAGTC
  CAAAAGGATGTGGTCATGGAACAAGCTGTTTCCTGTCCGGGTCCAGACTGGTCTTGGAGAGCAGCTTATC
  ATTCCTCCCTCAGAATTGGAGAGGTGTCCTGGTGCACCTTCCGTCTATGACATTCAGCTGAACCAGGTGT
  CACCTGCTGACTTCACTGTCCTCAGTGATGTGCTGCCAATGTTCAGCGTGGACTTCAGCAAGCAAGTCAG
  CAGCTCGGCAGCGTGCCATAGCAAGCAGTTTGTCCCTCTGGCATCTGGCCAAGCACAGGTGGTTCTCTCC
  TGGTGGGACATAGAAATGGACCCTGAGGGCAAGATCAAGTGCACCATGGCACCCTTTTGGGCACAGACAG
  ATCCACAGGAACTTCAGTGGCGGGACCACTGGATGCAGTGTGTGTACTTCCTGCCCCAGGAGGAGCCCAT
  TATGCAGGGCTCACCCCGATGCCTGGCTGCTCACCATGATGACTACTGTGTGTGGTACAGCCTTCAGAGA
  ACCAGCCCTGATGAGAATAACAGTGCCTACCAAGTTCGACCTGTATGTGACTGTCAGGCTCACCTGCTCT
  GGAACCGGCCACGGTTTGGAGAGATCAATGATCAGGACAGAACTGATCACTATGCCCGAGCCCTGAGGAC
  TATGCTGATGCCAGGTAGCATCTGCCTTTGTGTGAGTGACGGCAGTCTACTCTCTGTGTTGGCCCATCAC
  CTTGGAGCAGAGCAGGTGTTTACAGTCGAGAGTTCAGTAGCTTCCTATAGACTGATGAAAAGGATCTTCA
  AGGTTAACCACTTGGAAGATAAAATTACCGTCATCAATAAACGGCCTGAGTTGTTGACATCTGCAGACCT
  GGAGGGTAAGAAGGTCTCCCTCCTCCTGGGTGAACCTTTTTTCACCACCAGCCTGCTACCATGGCACAAC
  CTGTACTTCTGGTATGTGCGCACCTCTGTGGACCAGCACCTAGCGCCTGGGGCTGTGGTGATGCCCCAGG
  CAGCCTCACTGCATGCCGTGATCGTGGAGTTCAGGGACCTGTGGCGAATCCGGAGTCCTTGTGGTGACTG
  TGAAGGGTTTGACGTCCACATCATGGATGATATGATCAAGCACTCCCTGGATTTCCGAGAGAGCAGGGAG
  GCAGAGCCACAGCCACTGTGGGAATACCCCTGCAGAAGCCTCTCAGAGCCTCGACAGATCCTGACTTTTG
  ATTTCCAGCAGCCCATCCCCCAGCAGCCTATGCAATCCAGGGGCGTGATGGAACTGAGGAGGCCCGGGAA
  GAGCCATGGAGCTGTCCTGTGGATGGAATACCAGCTGACTCCAGATAGCACTGTCAGCACCGGCCTCATG
  AACCCTGCAGAGGACAAGGGGGACTGCTGCTGGAACCCCCACTGCAAGCAAGCTGTGTACTTCCTCAGTG
  CCACACTGGATCCTAGCGCGCCTCTGGATGGCCCTCAATCAGTCAGCTATGCTGTGGAGTTTCACCCCCT
  CACTGGAGACATCACCATGGAGTTTAGGCTTGCAGACGACACCTTGAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214544 representing NM_001014153
  Red=Cloning site Green=Tags(s)
  
  MKVFCGRANPTTGSLEWLEEDEHYDYHQEIARSSYADMLHDKDRNIKYYQGIRAAVSRVKDKGQKALVLD
  IGTGTGLLSMMAVTAGADFCYAVEVFKPMAEAAVKIVEKNGFSDKIKVINKHSTEVTVGPDGDLPCRANI
  LVTELFDTELIGEGALPSYEHAHKHLVQEDCEAVPHRATVYAQLVESKRMWSWNKLFPVRVQTGLGEQLI
  IPPSELERCPGAPSVYDIQLNQVSPADFTVLSDVLPMFSVDFSKQVSSSAACHSKQFVPLASGQAQVVLS
  WWDIEMDPEGKIKCTMAPFWAQTDPQELQWRDHWMQCVYFLPQEEPIMQGSPRCLAAHHDDYCVWYSLQR
  TSPDENNSAYQVRPVCDCQAHLLWNRPRFGEINDQDRTDHYARALRTMLMPGSICLCVSDGSLLSVLAHH
  LGAEQVFTVESSVASYRLMKRIFKVNHLEDKITVINKRPELLTSADLEGKKVSLLLGEPFFTTSLLPWHN
  LYFWYVRTSVDQHLAPGAVVMPQAASLHAVIVEFRDLWRIRSPCGDCEGFDVHIMDDMIKHSLDFRESRE
  AEPQPLWEYPCRSLSEPRQILTFDFQQPIPQQPMQSRGVMELRRPGKSHGAVLWMEYQLTPDSTVSTGLM
  NPAEDKGDCCWNPHCKQAVYFLSATLDPSAPLDGPQSVSYAVEFHPLTGDITMEFRLADDTLN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001014153
• ORF Size: 2079 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001014153.1, NP_001014175.1
• RefSeq Size: 2325 bp
• RefSeq ORF: 2082 bp
• Locus ID: 361402
• Cytogenetics: 19q12
• MW: 78.3 kDa