Alg8 (NM_001034127) Rat Tagged ORF Clone

CAT#: RR214697

Alg8 (Myc-DDK-tagged ORF) - Rat asparagine-linked glycosylation 8, alpha-1,3-glucosyltransferase homolog (S. cerevisiae) (Alg8), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Alg8
• Synonyms: MGC124750
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214697 representing NM_001034127
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGTTTGGGCTTGCAGCCGGTAGTGGCCATTGGTTCTCGGCTTTGGCTCTTGGGGTGACACTCC
  TTAAGTGCCTTCTCATCCCCACCTACCATTCCACAGATTTTGAAGTGCACCGAAATTGGCTTGCTATCAC
  TCACAACCTCCCAATATCTCAGTGGTATTATGAGGCAACTTCAGAGTGGACCTTGGATTATCCCCCCTTT
  TTTGCATGGTTCGAGTACGCCTTATCACATATTGCCAAATACTTTGATCAAGAGATGCTTGATATCCATA
  ACCTGAACTATTACAGCTCAAGGACACTGCTTTTCCAGAGGTTTTCTGTCATCTTGACAGATGCGCTCTT
  TGTGTATGCTGTTCACGAGTGCTGTAAATGCATCGACGGAAAAAGAACAGGGAAAGACCCCACAGAGAAA
  CCGAAGTTTGTTCTGTCCGTGCTATTGCTGTGGAACTTCGGGTTGTTAATTGTGGACCACATTCATTTCC
  AGTACAATGGCTTGCTCTCTGGCTTGCTGCTGCTGTCCATTGCCCGTCTGTTTCAGAAGAGGCATATTGA
  AGGGGCATTTCTCTTTGCTGTCCTCCTCCATTTCAAGCACATCTACCTCTATGTAGCCCCAGCCTATGGT
  ATCTATCTGCTGCGATCTTACTGTTTCACGGCAAGTAAACCAGATGGCTCTGTCCGATGGGACAGCTTCA
  GCATTGTCCGAGTTACTGCCCTGGCACTGATCGTTTTCCTAGTTTCCGCTCTTTCGCTAGGTCCTTTCCT
  AGCCTTGAACCAGCTGCCACAAGTCTTCTCCCGACTCTTTCCTTTCAAGAGGGGCCTTTGCCATGCCTAC
  TGGGCTCCAAACTTCTGGGCCTTGTACAGTGCTTTGGACAAAGCACTGTGTGTAACTGGTTTGGAACTGA
  AGCTTCTTGATCCCAGCCAGATCCCCAGGGCCTCAATGACAAGTGGTTTGGTCCAGCAGTCCCAGCACAC
  TGTCCTCCCCTCAGTCTCCCCCTCGGCAACCCTCATCTGCACACTGATAGCCATATTGCCCTCTGTTTTC
  TGTCTTTGGTTTAAACCCCAAGGGCCCAGAGGCTTTCTCCGATGCTTGGTTCTTTGTGCCTTAAGTTCCT
  TCATGTTTGGGTGGCATGTCCATGAAAAAGCCATACTCCTTGCGATTCTCCCAATGAGCCTACTGGCTGT
  GGAAAAAGCAGGAGATGCGACAATTTTTCTGATTCTGACGACAACCGGACACTATTCCCTCTTCCCTCTG
  CTCTTCACTGCACCAGAACTCCCCATTAAAATCTTGCTCATGTCCCTGTTCACTGTGTACAGTATCTCCT
  CGCTGAGGACGCTGTTCAGAAAAGAAAAACCTCTTTTTAATTGGATGGAAACTCTGTACCTCCTTGGCCT
  GGGGCCTCTGGAAGTCTGCTGTGAGTTTCTATTCCCTTTTACCTCGTGGAAGCTGAAGTACCCCTTCATC
  CCTTTGCTGCTGACCTCGGTGTACTGTGCAGTGGGCATCACGTACGCCTGGATCAGGCTGTATGCTTCAG
  TGTTGACTGGCTGTCTTGTCAGCAAGACAAAGAAGCAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214697 representing NM_001034127
  Red=Cloning site Green=Tags(s)
  
  MAAFGLAAGSGHWFSALALGVTLLKCLLIPTYHSTDFEVHRNWLAITHNLPISQWYYEATSEWTLDYPPF
  FAWFEYALSHIAKYFDQEMLDIHNLNYYSSRTLLFQRFSVILTDALFVYAVHECCKCIDGKRTGKDPTEK
  PKFVLSVLLLWNFGLLIVDHIHFQYNGLLSGLLLLSIARLFQKRHIEGAFLFAVLLHFKHIYLYVAPAYG
  IYLLRSYCFTASKPDGSVRWDSFSIVRVTALALIVFLVSALSLGPFLALNQLPQVFSRLFPFKRGLCHAY
  WAPNFWALYSALDKALCVTGLELKLLDPSQIPRASMTSGLVQQSQHTVLPSVSPSATLICTLIAILPSVF
  CLWFKPQGPRGFLRCLVLCALSSFMFGWHVHEKAILLAILPMSLLAVEKAGDATIFLILTTTGHYSLFPL
  LFTAPELPIKILLMSLFTVYSISSLRTLFRKEKPLFNWMETLYLLGLGPLEVCCEFLFPFTSWKLKYPFI
  PLLLTSVYCAVGITYAWIRLYASVLTGCLVSKTKKH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001034127
• ORF Size: 1578 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001034127.1, NP_001029299.1
• RefSeq Size: 2013 bp
• RefSeq ORF: 1581 bp
• Locus ID: 293129
• Cytogenetics: 1q32
• MW: 59.7 kDa