Arih1 (NM_001013108) Rat Tagged ORF Clone

CAT#: RR214713

Arih1 (Myc-DDK-tagged ORF) - Rat ariadne ubiquitin-conjugating enzyme E2 binding protein homolog 1 (Drosophila) (Arih1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Arih1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214713 representing NM_001013108
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTCGGACGAGGGCTACAACTACGAGTTCGACGAGGACGAGGAGTGCAGCGAGGAGGACAGCGGCG
  CCGAGGAGGAGGAGGACGACGACGAGGATGAGCCGGACGATGATAACCTGGATCTCGGCGAGGTGGAGCT
  GGTGGAGCCCGGGCTGGGCGTCGGCGGGGAGCGGGACGGACTGCTGTGCGGGGAGACTGGCGGCGGCGGC
  GGCAGCGCTCTGGGTCCCGGAGGCGGTGGCGGCGGTGGCGGTGGCGGCGGCGGCCCGGGGCACGAGCAGG
  AGGAAGATTACCGCTACGAGGTGCTCACGGCCGAACAGATCCTGCAGCACATGGTGGAATGTATCCGGGA
  GGTCAACGAGGTCATCCAGAATCCAGCAACTATCACGAGAATACTCCTTAGCCACTTCAATTGGGATAAA
  GAGAAGCTAATGGAAAGGTACTTTGATGGAAACCTGGAGAAGCTCTTTGCTGAGTGTCATGTAATTAATC
  CAAGTAAAAAGTCTCGAACTCGCCAGATGAATACAAGGTCATCAGCACAGGATATGCCTTGTCAGATCTG
  CTACTTGAATTACCCTAACTCGTATTTTACTGGCCTTGAATGTGGACATAAGTTTTGTATGCAGTGCTGG
  AGTGAATATTTAACTACCAAAATAATGGAAGAAGGCATGGGGCAGACTATCTCGTGTCCTGCGCATGGCT
  GTGATATCTTAGTGGATGACAACACAGTTATGCGCCTGATCACAGATTCAAAAGTTAAATTAAAATATCA
  GCATTTAATAACAAATAGCTTTGTAGAGTGCAACCGACTGTTAAAATGGTGTCCTGCCCCCGATTGCCAC
  CATGTTGTTAAAGTCCAGTATCCTGATGCAAAACCAGTTCGCTGCAAGTGTGGCCGCCAGTTTTGCTTTA
  ACTGTGGCGAAAATTGGCATGATCCAGTTAAATGTAAGTGGTTGAAGAAGTGGATTAAAAAGTGTGATGA
  TGACAGTGAAACTTCTAATTGGATTGCAGCTAACACAAAGGAATGTCCCAAATGCCATGTCACAATTGAG
  AAGGATGGTGGTTGTAATCACATGGTCTGTCGTAACCAGAACTGTAAAGCAGAGTTTTGCTGGGTGTGTC
  TTGGCCCTTGGGAACCACATGGATCTGCCTGGTACAACTGTAACCGCTATAATGAGGATGATGCAAAGGC
  AGCAAGAGATGCCCAAGAGCGATCTAGGGCAGCCCTGCAGAGGTACCTGTTCTACTGTAATCGCTATATG
  AACCACATGCAGAGTCTACGCTTTGAGCATAAGCTGTATGCTCAGGTGAAACAGAAGATGGAGGAAATGC
  AGCAGCACAACATGTCCTGGATTGAGGTGCAGTTCCTGAAGAAAGCAGTTGATGTCCTCTGCCAGTGTCG
  TGCCACACTCATGTACACTTATGTCTTCGCTTTCTACCTCAAAAAGAATAACCAGTCCATTATCTTTGAG
  AATAATCAAGCAGATCTAGAAAATGCCACAGAGGTGCTTTCGGGCTACCTTGAACGAGATATTTCCCAAG
  ATTCTCTGCAAGATATAAAGCAGAAAGTCCAAGATAAGTACAGATACTGTGAGAGTCGACGAAGGGTTTT
  GTTACAGCATGTGCATGAAGGCTATGAAAAAGATCTGTGGGAGTACATTGAGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214713 representing NM_001013108
  Red=Cloning site Green=Tags(s)
  
  MDSDEGYNYEFDEDEECSEEDSGAEEEEDDDEDEPDDDNLDLGEVELVEPGLGVGGERDGLLCGETGGGG
  GSALGPGGGGGGGGGGGGPGHEQEEDYRYEVLTAEQILQHMVECIREVNEVIQNPATITRILLSHFNWDK
  EKLMERYFDGNLEKLFAECHVINPSKKSRTRQMNTRSSAQDMPCQICYLNYPNSYFTGLECGHKFCMQCW
  SEYLTTKIMEEGMGQTISCPAHGCDILVDDNTVMRLITDSKVKLKYQHLITNSFVECNRLLKWCPAPDCH
  HVVKVQYPDAKPVRCKCGRQFCFNCGENWHDPVKCKWLKKWIKKCDDDSETSNWIAANTKECPKCHVTIE
  KDGGCNHMVCRNQNCKAEFCWVCLGPWEPHGSAWYNCNRYNEDDAKAARDAQERSRAALQRYLFYCNRYM
  NHMQSLRFEHKLYAQVKQKMEEMQQHNMSWIEVQFLKKAVDVLCQCRATLMYTYVFAFYLKKNNQSIIFE
  NNQADLENATEVLSGYLERDISQDSLQDIKQKVQDKYRYCESRRRVLLQHVHEGYEKDLWEYIED
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001013108
• ORF Size: 1665 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001013108.2, NM_001013108.3, NP_001013126.2
• RefSeq Size: 5130 bp
• RefSeq ORF: 1668 bp
• Locus ID: 300756
• Cytogenetics: 8q24
• MW: 64 kDa