Rrm1 (NM_001013236) Rat Tagged ORF Clone

CAT#: RR214738

Rrm1 (Myc-DDK-tagged ORF) - Rat ribonucleotide reductase M1 (Rrm1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rrm1
• Synonyms: MGC94742
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214738 representing NM_001013236
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATGTGATCAAGCGAGATGGCCGCCAAGAGCGAGTTATGTTTGACAAAATTACATCCCGAATCCAGA
  AACTCTGTTATGGACTCAATATGGACTTTGTGGATCCTGCTCAGATCACCATGAAAGTAATCCAAGGCCT
  ATACAGTGGGGTCACCACAGTGGAACTGGACACCCTGGCTGCTGAGACAGCTGCCACCTTGACTACGAAG
  CACCCTGACTATGCCATCCTGGCAGCAAGGATCGCTGTCTCTAACTTGCACAAGGAAACAAAGAAAGTGT
  TCAGTGACGTGATGGAGGATCTCTACAACTACATAAATCCACACAACGGCAGACATTCTCCCATGGTGGC
  CAGCTCAACACTCGAGATTGTTATGGCCCATAAGGATCGCCTGAATTCTGCCATTATCTATGACCGGGAT
  TTCTCTTACAACTACTTTGGTTTTAAGACACTGGAACGGTCATATTTGTTGAAGATCAATGGAAAAGTGG
  CTGAAAGACCACAGCACATGTTGATGAGGGTATCTGTGGGGATTCACAAAGAAGATATTGATGCTGCAAT
  TGAAACATACAATCTACTTTCTGAGAAGTGGTTTACTCACGCCTCTCCGACTCTCTTCAATGCTGGGACC
  AACCGCCCACAGTTGTCCAGCTGTTTCCTCTTGAGTATGAAAGATGACAGCATTGAGGGGATTTATGATA
  CTCTGAAGCAGTGTGCCTTGATTTCTAAGTCTGCTGGAGGAATTGGTGTTGCCGTGAGTTGTATTCGGGC
  CACTGGCAGCTACATTGCTGGGACTAATGGCAATTCTAATGGCCTTGTGCCAATGCTGAGAGTCTATAAC
  AACACAGCTCGTTATGTGGATCAAGGTGGAAACAAGCGCCCAGGGGCATTTGCTATTTACCTGGAGCCTT
  GGCACCTGGACATCTTTGAGTTTCTTGACTTGAAGAAGAACACAGGCAAGGAAGAACAGCGCGCGCGGGA
  TCTCTTCTTTGCACTGTGGATCCCAGATCTCTTCATGAAGCGAGTGGAGACCAACCAGGACTGGTCACTG
  ATGTGTCCCAATGAGTGTCCTGGTCTGGACGAGGTCTGGGGAGAGGAGTTTGAGAAGTTATATGAAAGTT
  ACGAGAAGCAGGGCCGTGTCCGAAAAGTTGTGAAGGCTCAGCAGCTTTGGTACGCCATCATTGAGTCTCA
  GACGGAGACGGGCACCCCATACATGCTCTACAAAGACTCCTGTAACCGGAAGAGCAACCAGCAGAACCTG
  GGAACCATCAAGTGCAGCAACCTGTGCACAGAGATAGTAGAGTACACCAGTAAAGATGAGGTTGCGGTTT
  GTAACTTGGCTTCTCTGGCTCTGAACATGTATGTCACACCAGAACACACGTATGACTTTGAGAAACTGGC
  AGAAGTCACTAAAGTCATTGTCCGAAATCTGAATAAAATAATTGATATAAACTACTATCCTATTCCAGAG
  GCACACTTATCAAATAAACGCCATCGGCCCATTGGAATTGGGGTACAAGGTCTAGCAGATGCTTTCATCC
  TGATGAGGTATCCCTTTGAGAGCCCAGAAGCCCAGCTACTAAATAAGCAAATCTTTGAAACCATCTATTA
  TGGAGCCCTGGAAGCCAGCTGTGACCTAGCCAAGGAGTATGGCCCCTACGAAACGTATGAGGGATCTCCA
  GTCAGCAAGGGTATTCTTCAGTATGATATGTGGAATGTTACTCCTACAGACCTGTGGGACTGGAAGCTTC
  TCAAGGAGAAGATTGCAAAGTACGGTATAAGAAACAGTTTACTTATTGCCCCAATGCCTACTGCTTCAAC
  TGCTCAGATTCTGGGGAATAATGAGTCCATTGAGCCTTACACCAGTAACATCTACACTCGCAGAGTCTTG
  TCAGGAGAATTTCAGATTGTGAATCCTCACTTACTGAAAGATCTTACTGAGCGGGGCTTGTGGAATGAAG
  AGATGAAAAATCAGATTATTGCCTGCAATGGCTCCATTCAGAGCATACCAGAAATTCCTGAGGACCTGAA
  GCAGCTCTATAAGACCGTGTGGGAAATCTCTCAGAAGACTGTTCTCAAGATGGCAGCCGAGAGAGGTGCT
  TTCATCGATCAAAGCCAGTCTTTAAACATCCATATCGCTGAGCCCAACTATGGCAAACTCACTAGTATGC
  ACTTCTACGGTTGGAAGCAGGGTTTAAAGACTGGGATGTATTATTTAAGGACAAGACCTGCCGCTAATCC
  AATCCAGTTCACTCTGAACAAGGAAAAGCTGAAAGATAAGGAAAAGGCACTGAAGGAGGAAGAAGAGAAG
  GAGAGGAACACAGCAGCCATGGTGTGCTCTTTGGAGAACAGAGAGGAGTGTCTGATGTGTGGATCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214738 representing NM_001013236
  Red=Cloning site Green=Tags(s)
  
  MHVIKRDGRQERVMFDKITSRIQKLCYGLNMDFVDPAQITMKVIQGLYSGVTTVELDTLAAETAATLTTK
  HPDYAILAARIAVSNLHKETKKVFSDVMEDLYNYINPHNGRHSPMVASSTLEIVMAHKDRLNSAIIYDRD
  FSYNYFGFKTLERSYLLKINGKVAERPQHMLMRVSVGIHKEDIDAAIETYNLLSEKWFTHASPTLFNAGT
  NRPQLSSCFLLSMKDDSIEGIYDTLKQCALISKSAGGIGVAVSCIRATGSYIAGTNGNSNGLVPMLRVYN
  NTARYVDQGGNKRPGAFAIYLEPWHLDIFEFLDLKKNTGKEEQRARDLFFALWIPDLFMKRVETNQDWSL
  MCPNECPGLDEVWGEEFEKLYESYEKQGRVRKVVKAQQLWYAIIESQTETGTPYMLYKDSCNRKSNQQNL
  GTIKCSNLCTEIVEYTSKDEVAVCNLASLALNMYVTPEHTYDFEKLAEVTKVIVRNLNKIIDINYYPIPE
  AHLSNKRHRPIGIGVQGLADAFILMRYPFESPEAQLLNKQIFETIYYGALEASCDLAKEYGPYETYEGSP
  VSKGILQYDMWNVTPTDLWDWKLLKEKIAKYGIRNSLLIAPMPTASTAQILGNNESIEPYTSNIYTRRVL
  SGEFQIVNPHLLKDLTERGLWNEEMKNQIIACNGSIQSIPEIPEDLKQLYKTVWEISQKTVLKMAAERGA
  FIDQSQSLNIHIAEPNYGKLTSMHFYGWKQGLKTGMYYLRTRPAANPIQFTLNKEKLKDKEKALKEEEEK
  ERNTAAMVCSLENREECLMCGS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001013236
• ORF Size: 2376 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001013236.1, NP_001013254.1
• RefSeq Size: 3038 bp
• RefSeq ORF: 2379 bp
• Locus ID: 685579
• Cytogenetics: 1q32
• MW: 90.3 kDa
• Gene Summary: putative ribonucleotide reductase enzyme; human homolog encodes an enzyme required for production of deoxyribonucleotides prior to DNA synthesis [RGD, Feb 2006]