Cad (NM_001105710) Rat Tagged ORF Clone

CAT#: RR214754

Cad (Myc-DDK-tagged ORF) - Rat carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cad
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214754 representing NM_001105710
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCCTGGTGTTGGAGGACGGGTCGGTGCTGCAGGGCCGGCCCTTCGGGGCCGCTGTGTCGACTG
  CTGGGGAAGTGGTGTTTCAGACCGGCATGGTCGGCTACCCGGAGGCCCTCACTGACCCTTCCTACAAAGC
  TCAGATCCTAGTGCTAACGTACCCTCTCATCGGCAACTACGGTATTCCCTCAGATGAAGAGGACGAGTTC
  GGCCTGAGCAAGTGGTTTGAATCCTCGGAGATCCATGTGGCAGGTCTGGTGGTTGGGGAATGCTGTCCCA
  CGCCCAGCCACTGGAGTGCCACCTGCACCCTGCATGAGTGGCTGCAACAGCATGGCATCCCCGGCCTGCA
  AGGAGTGGACACTCGGGAGCTGACTAAGAAGTTGCGGGAACAAGGGTCTCTTTTGGGGAAGCTGGTCCAG
  AAAGGGACAGAGCCTTCAGCCCTGCCATTTGTGGACCCCAATGCCAGGCCCCTGGCACCAGAGGTCTCTA
  TTAAGACCCCACGGGTATTCAATGCGGGGGGTGCCCCTCGGATCTTTGCTGTAGACTGTGGCCTCAAGTA
  CAATCAGATCCGATGTCTCTGCCAGCTTGGGGCTGAGGTTACCGTGGTACCCTGGAACCATGAATTAGAC
  AGTCGGAAATATGACGGTCTCTTCCTAAGTAATGGACCTGGTGATCCTGCCTCCTATCCTGGTGTGGTGT
  CCACACTGAATCGAGTCTTATCTGAGCCTAATCCCCGACCTGTGTTTGGAATCTGCCTTGGACACCAGCT
  ATTGGCTTTAGCCATTGGGGCCAAAACTTACAAGATGAGATACGGAAACCGAGGCCACAACCAGCCCTGT
  TTACTGGTGGGTACTGGGCGCTGCTTTCTGACCTCCCAGAATCACGGGTTTGCCGTGGACGCGGATTCGC
  TGCCAGCAGGCTGGGCTCCTCTATTTACCAACGCCAATGACTGTTCCAACGAAGGCATTGTACACGACAA
  TCTGCCCTTCTTCAGCGTCCAGTTTCACCCCGAGCACCGGGCTGGCCCTTCAGATATGGAGCTACTCTTC
  GATGTGTTTCTAGAAACCGTGAGAGATACTGTAGCTGGGAACGCCGAGGGCCGGACAGTAAGAGAACGTT
  TGGCTCAGCGCCTCTGTCCCCCTGAGCTTCCCATTCCTGGTTCTGGGCTCCCACCACCACGAAAAGTTCT
  GATCCTGGGCTCGGGGGGCCTCTCCATTGGCCAGGCTGGAGAATTTGACTACTCGGGCTCTCAGGCAATT
  AAAGCCCTAAAGGAGGAGAACATCCAAACATTACTGATCAACCCCAACATTGCCACAGTGCAGACTTCTC
  AGGGGCTGGCAGACAAGGTCTATTTCCTTCCCATAACACCTCACTACGTAACCCAGGTGATTCGTAATGA
  ACGCCCAGATGGTATATTACTGACTTTCGGGGGTCAGACAGCCCTGAACTGCGGTGTGGAGCTGACCAAA
  GCTGGGGTACTAGCTCGGTATGGGGTCCGGGTCTTGGGTACACCTGTGGAGACCATTGAACTGACTGAGG
  ACCGGCGAGCCTTTGCTGCCAGAATGGCTGAGATTGGAGAGCACGTTGCCCCCAGTGAAGCTGCAAATTC
  TCTTGAACAGGCTCAGGCAGCTGCCGAGCGGCTAGGCTACCCTGTGCTGGTGCGAGCAGCCTTTGCCCTG
  GGTGGTCTTGGTTCTGGCTTTGCTTCCACCAAAGAGGAACTCTCAGCTCTTGTGGCTCCAGCCTTTGCCC
  ATACCAGCCAGGTGCTGATAGACAAGTCTCTGAAGGGCTGGAAGGAGATTGAATATGAGGTGGTGAGAGA
  TGCCTATGGCAACTGCGTAACGGTATGTAACATGGAGAACTTAGACCCACTGGGCATCCATACGGGGGAG
  TCCATAGTGGTGGCTCCAAGCCAGACACTGAATGACAGAGAGTACCAGCTTCTGCGGCGGACAGCTATCA
  AGGTCACCCAGCACCTGGGGATTGTTGGGGAGTGCAATGTGCAGTATGCCTTGAACCCCGAGTCTGAGCA
  GTATTACATCATTGAAGTAAATGCCAGGCTCTCCCGAAGCTCTGCCCTGGCCAGTAAGGCCACAGGCTAT
  CCACTAGCCTATGTGGCAGCCAAGCTAGCATTGGGCATTCCCCTGCCAGAGCTCAGAAACTCTGTTACGG
  GGGGAACAGCAGCCTTTGAGCCCAGCCTGGACTACTGCGTGGTAAAGATTCCTCGCTGGGACCTCAGCAA
  GTTCCTGCGTGTCAGTACCAAGATTGGGAGCTGCATGAAGAGTGTTGGTGAAGTCATGGGCATCGGGCGT
  TCCTTTGAGGAGGCCTTCCAAAAGGCCCTGCGCATGGTAGACGAGAACTGTGTAGGCTTCGACCATACAG
  TGAAGCCAGTCAGCGACGTGGAGCTGGAGACGCCGACGGATAAGCGGATCTTTGTGGTGGCTGCTGCTCT
  GTGGGCTGGCTACTCTGTGGAGCGCCTCTACGAGCTCACCCGCATCGACTGCTGGTTCCTGCACCGGATG
  AAGCGCATTGTGACCCACGCCAAGTTGCTGGAACAACACCGTGGACGGCCCTTACCCCAAGACCTGCTGC
  ACCAGGCCAAGTGCCTCGGCTTCTCAGACAAGCAAATTGCTCTTGCAGTCCTGAGCACAGAGCTGGTTGT
  TCGGAAGCTGCGTCAGGAACTGGGGATCTGCCCAGCAGTGAAGCAGATCGACACAGTTGCAGCTGAGTGG
  CCAGCCCAGACAAATTACCTGTACCTGACATACTGGGGCAACACCCATGACCTCGACTTTCGGACACCTC
  ACGTCCTGGTCCTTGGCTCTGGCGTCTACCGGATCGGCTCCAGCGTTGAGTTTGATTGGTGTGCTGTGGG
  CTGCATCCAGCAGCTCCGGAAGATGGGTTATAAGACCATCATGGTGAACTACAACCCAGAGACAGTCAGC
  ACCGACTATGATATGTGTGACCGACTCTACTTTGATGAGATCTCTTTTGAGGTGGTGATGGACATCTATG
  AGTTAGAAAACCCCGAAGGTGTGATTCTGTCCATGGGCGGGCAGCTGCCCAACAACATGGCTATGGCTTT
  GCATCGGCAGCAGTGCCGAGTGCTGGGCACCTCCCCCGAAGCCATCGACTCAGCCGAGAACCGGTTCAAG
  TTCTCCCGGCTCCTAGATACCATTGGTATCAGCCAGCCTCAGTGGCGAGAGCTCAGTGACCTCGAGTCCG
  CTCGCCAGTTCTGCCAGACCGTTGGGTACCCCTGCGTAGTGCGTCCCTCCTATGTGCTGAGTGGTGCTGC
  TATGAACGTGGCCTACACTGATGGGGACCTGGAGCGCTTCCTGAGCAGTGCAGCTGCTGTCTCTAAGGAG
  CACCCTGTGGTCATCTCCAAATTCATTCAGGAGGCAAAGGAGATTGATGTAGATGCTGTGGCCTGTGACG
  GCATCGTGTCGGCTATTGCCATCTCTGAGCACGTGGAGAATGCAGGTGTGCATTCAGGGGATGCCACGCT
  GGTGACCCCCCCACAAGACATCACCCCCAAAACTCTGGAGCGGATCAAAGCCATTGTGCATGCTGTGGGC
  CAGGAGCTACAGGTCACGGGGCCCTTCAATCTGCAGCTCATTGCCAAGGACGACCAGCTGAAGGTCATTG
  AATGTAACGTGCGGGTGTCGCGCTCCTTCCCGTTCGTGTCCAAGACACTTGGTGTCGACCTCGTAGCCTT
  GGCCACAAGGATCATCATGGGGGAGAAAGTAGAGTCCGTGGGGCTCATGACTGGCTCTGGAGTCGTGGGA
  GTAAAGGTACCTCAGTTCTCCTTCTCACGCTTGGCCGGTGCTGACGTGGTCCTGGGTGTGGAGATGACCA
  GTACTGGAGAGGTGGCTGGCTTCGGAGAGAGCCGTTGTGAAGCCTACCTCAAAGCCATGCTCAGCACTGG
  CTTTAAGATCCCAAAGAAGAACATCCTGCTGACCATTGGCAGCTACAAGAATAAAAGTGAGCTGCTCCCA
  ACTGTGCGGTTGCTGGAGAGCCTGGGCTACAGTCTGTATGCCAGCTTGGGAACTGCCGACTTCTACACCG
  AGCACGGCGTCAAGGTAACAGCTGTGGACTGGCACTTTGAAGAGGCTGTGGATGGTGAGTGCCCACCACA
  ACGGAGCATCTTGGATCAGCTGGCCGAGAATCACTTTGAGCTGGTGATTAACCTGTCGATGCGCGGGGCT
  GGGGGTCGGCGTCTTTCCTCCTTCGTTACCAAGGGCTACCGCACCCGGCGTCTGGCCGCGGATTTCTCCG
  TGCCTCTCATCATTGATATCAAGTGCACCAAACTCTTCGTGGAGGCCCTAGGCCAGATCGGGCCAGCCCC
  TCCTTTGAAAGTCCACGTTGACTGCATGACCTCCCAGAAGCTAGTGCGCCTGCCTGGATTGATTGATGTC
  CACGTGCACCTTCGGGAACCAGGTGGGACTCACAAAGAGGACTTTGCCTCAGGCACAGCTGCCGCCTTGG
  CTGGGGGCGTCACCATGGTTTGTGCTATGCCTAATACCCGGCCCCCCATCATTGATGCCCCAGCTCTGGC
  GCTTGCCCAGAAGCTGGCAGAGGCTGGCGCCCGCTGTGACTTTACCCTCTTCCTCGGAGCCTCATCTGAA
  AATGCAGGGACTCTGGGTGCTGTGGCTGGGTCTGCAGCAGGCCTGAAGCTCTACCTCAATGAGACCTTCT
  CTGAGCTACGGCTGGACAGTGTGGCCCAGTGGATGGAGCATTTTGAAACCTGGCCTTCCCACCTCCCCAT
  TGTGGCCCACGCAGAGCGGCAGAGTGTCGCTGCGGTCCTCATGGTGGCTCAGCTGACACGGCGTTCAGTG
  CACATATGTCATGTGGCTCGGAAGGAAGAGATCCTGCTGATTAAAACTGCAAAGGCACAGGGGCTGCCAG
  TGACGTGTGAGGTCGCACCTCACCACCTGTTCCTAAACCGGGAAGACTTGGAGCGCCTGGGACCTGGGAA
  GGGAGAGGTCCGCCCTGAGCTGGGTTCCCGAGAGGATATGGAAGCCCTGTGGGAGAATATGGCGGTCATC
  GACTGTTTTGCCTCAGACCACGCTCCCCATACCTTGGAAGAGAAGTGTGGGCCTAAGCCTCCACCAGGCT
  TTCCAGGACTAGAGACCATGTTGCCGCTGCTACTGACAGCTGTGAGTGAAGGCCGCCTCAGTCTGGATGA
  CCTGCTACAGCGATTGCACCACAACCCTCGGCGGATCTTCCACCTGCCTCTCCAGGAGGACACCTATGTA
  GAGGTGGATCTGGAGCATGAGTGGACGGTCCCCAGCCACATGCCCTTCTCTAAAGCCCACTGGACCCCAT
  TTGAAGGTCAGAAGGTGAAGGGCACCGTGCGCCGTGTGGTCTTGAGAGGGGAGGTCGCCTATATTGATGG
  ACAGGTGCTGGTACCCCCGGGCTATGGACAAGATGTACGGAAGTGGCCTCAGGGGGCTGTTCCTCAGCCT
  CCTCCTCCAACTCCTACTACCACTGAAATAACCACGACACCTGAGAGACCACGCCGAGTCATCCCAGGCC
  TTCCTGATGGCCGATTCCACCTGCCACCTCGAATCCACCGGGCCTCTGACCCAGGGCTGCCAGCTGAGGA
  ACCCAAAGAGAAGCCACCCAGGAAGGTAGCAGAATCAGAGCTGATAGGAACCCCTGATGGCCCCTGCTAC
  CCTGTACCACCAGTACCTAGACAGGCATCACCACAGAATCTAGGATCTTCTGGCCTGCTGCACCCCCAGA
  TGTCACCTTTACTGCACTCCCTAGTGGGCCAACACATCCTGTCTGTCAAGCAGTTCACTAAGGATCAGAT
  GTCTCATCTGTTCAACGTGGCGCACACACTACGGATGATGGTGCAGAAAGAACGGAGCCTCGACATCCTA
  AAGGGGAAGGTCATGGCCTCTATGTTCTACGAGGTGAGCACCCGCACCAGTAGCTCCTTCGCAGCAGCCA
  TGGCCCGGCTCGGGGGTGCCGTTCTCAGCTTCTCTGAAGCCACGTCCTCCGTCCAGAAGGGCGAATCCCT
  CGCCGACTCGGTGCAGACCATGAGTTGCTATGCTGATGTTGTTGTACTCCGTCACCCTCAGCCTGGAGCT
  GTGGAGCTGGCGGCCAAGCACTGTCGAAGACCAGTGATCAATGCTGGAGATGGAGTTGGGGAGCACCCCA
  CTCAGGCCCTGCTGGACATCTTCACCATTCGAGAAGAGCTTGGGACTGTCAATGGCATGACGATCACTAT
  GGTAGGCGACCTGAAGCATGGACGCACAGTGCACTCCCTGGCCTGCCTGCTCACCCAGTACCGTGTGAGC
  CTACGCTACGTGGCACCTCCCAGCCTGCGCATGCCATCCAGCGTGTGGGACTTTGTGGCCTCCCGAGGCA
  CCAAGCAGGAGGAGTTCGAGAGCATCGAGGAGGCGCTGCCAGACACTGACGTGCTCTACATGACTCGGAT
  CCAGAAAGAACGATTTGGCTCTTCCCAGGAATATGAAGCTTGCTTTGGTCAGTTCATCCTCACTCCTCAC
  ATCATGACCCGGGCCAAGAAAAAGATGGTGGTGATGCACCCAATGCCTCGTGTCAATGAGATAAGCGTGG
  AGGTGGACTCGGACCCCAGAGCAGCCTATTTCCGCCAAGCTGAGAACGGCATGTACATCCGCATGGCTCT
  GCTTGCCACCGTGCTAGGCCGTTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214754 representing NM_001105710
  Red=Cloning site Green=Tags(s)
  
  MAALVLEDGSVLQGRPFGAAVSTAGEVVFQTGMVGYPEALTDPSYKAQILVLTYPLIGNYGIPSDEEDEF
  GLSKWFESSEIHVAGLVVGECCPTPSHWSATCTLHEWLQQHGIPGLQGVDTRELTKKLREQGSLLGKLVQ
  KGTEPSALPFVDPNARPLAPEVSIKTPRVFNAGGAPRIFAVDCGLKYNQIRCLCQLGAEVTVVPWNHELD
  SRKYDGLFLSNGPGDPASYPGVVSTLNRVLSEPNPRPVFGICLGHQLLALAIGAKTYKMRYGNRGHNQPC
  LLVGTGRCFLTSQNHGFAVDADSLPAGWAPLFTNANDCSNEGIVHDNLPFFSVQFHPEHRAGPSDMELLF
  DVFLETVRDTVAGNAEGRTVRERLAQRLCPPELPIPGSGLPPPRKVLILGSGGLSIGQAGEFDYSGSQAI
  KALKEENIQTLLINPNIATVQTSQGLADKVYFLPITPHYVTQVIRNERPDGILLTFGGQTALNCGVELTK
  AGVLARYGVRVLGTPVETIELTEDRRAFAARMAEIGEHVAPSEAANSLEQAQAAAERLGYPVLVRAAFAL
  GGLGSGFASTKEELSALVAPAFAHTSQVLIDKSLKGWKEIEYEVVRDAYGNCVTVCNMENLDPLGIHTGE
  SIVVAPSQTLNDREYQLLRRTAIKVTQHLGIVGECNVQYALNPESEQYYIIEVNARLSRSSALASKATGY
  PLAYVAAKLALGIPLPELRNSVTGGTAAFEPSLDYCVVKIPRWDLSKFLRVSTKIGSCMKSVGEVMGIGR
  SFEEAFQKALRMVDENCVGFDHTVKPVSDVELETPTDKRIFVVAAALWAGYSVERLYELTRIDCWFLHRM
  KRIVTHAKLLEQHRGRPLPQDLLHQAKCLGFSDKQIALAVLSTELVVRKLRQELGICPAVKQIDTVAAEW
  PAQTNYLYLTYWGNTHDLDFRTPHVLVLGSGVYRIGSSVEFDWCAVGCIQQLRKMGYKTIMVNYNPETVS
  TDYDMCDRLYFDEISFEVVMDIYELENPEGVILSMGGQLPNNMAMALHRQQCRVLGTSPEAIDSAENRFK
  FSRLLDTIGISQPQWRELSDLESARQFCQTVGYPCVVRPSYVLSGAAMNVAYTDGDLERFLSSAAAVSKE
  HPVVISKFIQEAKEIDVDAVACDGIVSAIAISEHVENAGVHSGDATLVTPPQDITPKTLERIKAIVHAVG
  QELQVTGPFNLQLIAKDDQLKVIECNVRVSRSFPFVSKTLGVDLVALATRIIMGEKVESVGLMTGSGVVG
  VKVPQFSFSRLAGADVVLGVEMTSTGEVAGFGESRCEAYLKAMLSTGFKIPKKNILLTIGSYKNKSELLP
  TVRLLESLGYSLYASLGTADFYTEHGVKVTAVDWHFEEAVDGECPPQRSILDQLAENHFELVINLSMRGA
  GGRRLSSFVTKGYRTRRLAADFSVPLIIDIKCTKLFVEALGQIGPAPPLKVHVDCMTSQKLVRLPGLIDV
  HVHLREPGGTHKEDFASGTAAALAGGVTMVCAMPNTRPPIIDAPALALAQKLAEAGARCDFTLFLGASSE
  NAGTLGAVAGSAAGLKLYLNETFSELRLDSVAQWMEHFETWPSHLPIVAHAERQSVAAVLMVAQLTRRSV
  HICHVARKEEILLIKTAKAQGLPVTCEVAPHHLFLNREDLERLGPGKGEVRPELGSREDMEALWENMAVI
  DCFASDHAPHTLEEKCGPKPPPGFPGLETMLPLLLTAVSEGRLSLDDLLQRLHHNPRRIFHLPLQEDTYV
  EVDLEHEWTVPSHMPFSKAHWTPFEGQKVKGTVRRVVLRGEVAYIDGQVLVPPGYGQDVRKWPQGAVPQP
  PPPTPTTTEITTTPERPRRVIPGLPDGRFHLPPRIHRASDPGLPAEEPKEKPPRKVAESELIGTPDGPCY
  PVPPVPRQASPQNLGSSGLLHPQMSPLLHSLVGQHILSVKQFTKDQMSHLFNVAHTLRMMVQKERSLDIL
  KGKVMASMFYEVSTRTSSSFAAAMARLGGAVLSFSEATSSVQKGESLADSVQTMSCYADVVVLRHPQPGA
  VELAAKHCRRPVINAGDGVGEHPTQALLDIFTIREELGTVNGMTITMVGDLKHGRTVHSLACLLTQYRVS
  LRYVAPPSLRMPSSVWDFVASRGTKQEEFESIEEALPDTDVLYMTRIQKERFGSSQEYEACFGQFILTPH
  IMTRAKKKMVVMHPMPRVNEISVEVDSDPRAAYFRQAENGMYIRMALLATVLGRF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001105710
• ORF Size: 6675 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001105710.2, NP_001099180.2
• RefSeq Size: 7126
• RefSeq ORF: 6678
• Locus ID: 24240
• MW: 243.5 kDa
• Gene Summary: important enzyme in the pathway of pyrimidine biosynthesis; may be regulated by the MAPK pathway [RGD, Feb 2006]