Nek1 (NM_001106082) Rat Tagged ORF Clone

CAT#: RR214766

Nek1 (Myc-DDK-tagged ORF) - Rat NIMA (never in mitosis gene a)-related expressed kinase 1 (Nek1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nek1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214766 representing NM_001106082
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAAGTATGTTAGACTACAGAAGATTGGAGAAGGTTCATTTGGAAAAGCTGTTCTTGTTAAATCTA
  CAGAGGATGGCAGACATTATGTCATCAAGGAAATTAACATCTCAAGAATGTCCGATAAAGAAAGAGAAGA
  ATCAAGGAGAGAGGTCGCAGTGTTGGCAAACATGAAGCATCCAAATATTGTCCAATACAAAGAATCATTC
  GAAGAAAATGGCTCTCTCTACATAGTAATGGATTACTGTGAAGGAGGTGATCTGTTTAAACGAATAAATG
  CTCAGAAAGGCACTTTGTTTCAAGAAGATCAGATTTTGGACTGGTTTGTTCAGATATGTTTGGCTTTGAA
  GCATGTACACGATAGAAAAATTCTTCACCGAGACATAAAGTCACAGAACATATTTCTAACCAAAGATGGG
  ACAGTGCAGCTTGGAGATTTTGGAATTGCTCGAGTTCTCAATAGTACTGTAGAGCTGGCTCGAACTTGCA
  TAGGGACTCCATACTACTTGTCACCTGAAATCTGTGAAAACAAGCCTTACAACAATAAAAGTGACATTTG
  GGCTTTGGGCTGTGTCCTTTATGAGTTGTGTACACTTAAACATGCATTTGAAGCTGGAAACATGAAAAAC
  CTGGTACTGAAGATAATATCTGGATCCTTTCCTCCAGTGTCTCTACATTATTCCTATGATCTCCGCAGCT
  TGCTCTCTCAGCTGTTTAAAAGAAATCCTAGGGATAGACCATCAGTCAACTCCATATTGGAGAAAGGCTT
  TATAGCTAAACGAATCGAAAAGTTTCTCTCACCTCAGCTTATTGCAGAAGAATTTTGTCTGAAAACAGTT
  TCAAAGTTTGGACCACAGCCTATCCCAGGTAAAAGACCAGCATCAGGACAAGGTGTCAGTTCTTTTGTCC
  CTGCTCAGAAAATCACAAAGCCTTCTGCTAAATATGGAGTGCCTATAACATATAAGAAGTACGGAGATAA
  AAAGTTACTTGAAAAAAAACCACCCCCAAAGCATAAGCAGGCCCATCAGATTCCAGTGAAGAAAATGAAT
  TCTGGAGAAGAAAGGAAGAAAATGTCTGAGGAAGCAGCAAAAAAACGAAGGTTGGAATTTATTGAGAAAG
  AAAAGAAGCAAAAGGATCAGATCAGGTTCCTGAAGGCTGAGCAGCTGAAGAGGCAAGAGAAGCAGCGGTT
  GGAGAGGATAAATAGGGCCAGGGAACAAGGATGGAGGAATGTTTTAAGGGCTGGTGGAAGCGGTGAAGTA
  AAGGCTTCCTTTTTTGGCAGTGGAGGAGCTGTGTCTCCATCACCTTGTTCTCCTCGGGGCCAGTATGAAC
  ATTACCATGCCATCTTTGACCAAATGCAGCAGCTAAGAGCAGAAGATAATGAAGCGAGATGGAAAGGGAG
  AATATATGGTCGATGGCTCCCTGAAAGGCAAAAAGGACACCTAGGTGTAGAGAGAGCCAAACAAGTGGAA
  GAATTCCTACAGCGTAAACAAGAAGCTATGCAGAATAAAGCCCGAGCAGAAGGACATGTGGTTTATCTGG
  CAAGACTGAGGCAAATAAGACTACAAAATTTTAATGAGCGCCAACAGATTAAAGCCAAACTTCGTGGTGA
  GAATAAAGAAGCTGGTGGTACCAAAGGACAAGACGCAACTGAAGAGGCTGACATGAGGCTCAAAAAGATG
  GAGTCACTTAAGGCCCAAGCAAATGCACGTGCTGCTGTACTAAAAGAACAGCTAGAGCGAAAAAGAAAGG
  AAGCTTATGAAAGAGAAAAGAAAGTGTGGGAAGAACATTTGGTGGTGAGGGTAAAAAGCTCAGATGTCCC
  TCCATCTATGGAGCATCATGAAGCAGGTGGTTCTCCATCACAGCAGCAGATGAAACCTGTTATTTCTGTG
  ACTTCAGCTTTGAAAGAAGTGGGCCTGGATGGAAGTTTAACTGAGTCCCAAGAAACTTCAGAAGAAATGG
  AAAAGAGTAACAATGCTATTTCAAGTAAGCGAGAAATACTGCGTAGGCTGAATGAAAATCTTAAAGCTCA
  AGAGGATGAAAAGGAAAAGCATCATCACGCTGATTCTTGTGAGACCGTTGGTCACAGAGATGAGAGAGAG
  TATGAGAAGGAAAATGCCATTTCCTCTGATCGCAAGAAATGGGAGATGGGAGGTCAGCTTGTGATTCCTC
  TGGATGAAGTGACACTGGATGCATCCTTCTCTGCTTCTGAAAAACATACTGTGGGAGAGGTTATTAAGTT
  AGATTCTAGTGGCTCTCCAAGAAAAGTCTGGGGGAAAAGCCCTACAGATTCTGTGCTGAAGATACTTGGA
  GAAGCTGAATTACAGCTACAGACAGAACTACTAGAAAACACGCCTTTTAAAAGTGAGGTTTGTGTTGAAG
  AGGAGAACTACAAACCCCTACTCACTGGAGAAGAGAATCTGCAGTGTATTTCAAAAGAAGTAAACCCATC
  AGCCACTGTTGATACTGAAACAAACAGTCCAAAGTTTGCTAAGGTGTCTCCAGCAATGTCCGAAGGAAAT
  GTGGAAGAACCTGATGATTTGGAAACAGAAGTTCTACAAGAGCCAAGTGCCATACCCTCAGATGGGAGTT
  TGCCGCCTGTTGTTAATGAGGTGTGGACCAGAGCTAAGGCAACTGGGTTGGAGGATAGCATTGCTGTGCA
  GCAGAATGACGTTTGTGAAGAGCGAATTCCAGGGACTGTGGACCAGTCCTGTAAGGATCATATAGAACCT
  GCAGGAAACGATTCTCCGCAGTCTGGCTGTGATGAAGAGAAGTCAGTACAGCCAGAATTGTTTTTCCAGA
  AAGTGGTTCACTCCAAGGACTTGAACTTAGTTCAGTCAGCTCATCGTTCCCCAGAAGAAGCAATTCCAAT
  TCGATCTCACTCTGATTCTCCACCAAAAGCTAAAAGCAAGAATTCCCTACTGATTGGACTTTCCACTGGT
  CTGTTTGATGCAAACAACCCAAAGATGCTGAGGACCTGTTCACTTCCAGACCTTTCCAAGCTCTTCAGAA
  CCCTAATGGATGTTCCCACTGTGGGAGACGTTCATCAAGACAGTCTTGAAATAGAGGAGCTTGAGGATGA
  ACATATTAAAGAAGGGCCCTCGGATTGCGAGGACATTGTATTTGAAGAAACTGACACAGATTTACAGGAG
  CTTCAGGCCTCAATGGAGCAGCTGCTTAGGGAGCAACCCGGTGACGAATACAGTGAGGAGGAAGAGTCTG
  TCTTAAAAAGCAGCAATGTGGAGCGGGCAGCAAGAGGGACAGATGTCCCAGATGAGGAGGACAACCCCAG
  CAGTGAAAGCGCCCTAAACGAGGAATGGCACTCAGATAACAGTGATGCTGAGACTACTAGTGAGTGTGAA
  TATGACAGTGTCTTTAACCATTTAGAGGAACTAAGACTTCACTTGGAACAAGAAATGGGCTTTGAAAAGT
  TCTTTGAGGTCTATGAGAAAGTAAAGGCTATTCATGAGGATGAAGATGAAAATATTGAAATTTCTTCAAC
  CATAGTCGAGAACATCTTGGGCACTGAGCATCAGCACCTTTATGCCAAGATTCTGCATCTAGTCATGGCA
  GACGGAGCCTATCAGGAAGATAATGATGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214766 representing NM_001106082
  Red=Cloning site Green=Tags(s)
  
  MEKYVRLQKIGEGSFGKAVLVKSTEDGRHYVIKEINISRMSDKEREESRREVAVLANMKHPNIVQYKESF
  EENGSLYIVMDYCEGGDLFKRINAQKGTLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDG
  TVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGNMKN
  LVLKIISGSFPPVSLHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTV
  SKFGPQPIPGKRPASGQGVSSFVPAQKITKPSAKYGVPITYKKYGDKKLLEKKPPPKHKQAHQIPVKKMN
  SGEERKKMSEEAAKKRRLEFIEKEKKQKDQIRFLKAEQLKRQEKQRLERINRAREQGWRNVLRAGGSGEV
  KASFFGSGGAVSPSPCSPRGQYEHYHAIFDQMQQLRAEDNEARWKGRIYGRWLPERQKGHLGVERAKQVE
  EFLQRKQEAMQNKARAEGHVVYLARLRQIRLQNFNERQQIKAKLRGENKEAGGTKGQDATEEADMRLKKM
  ESLKAQANARAAVLKEQLERKRKEAYEREKKVWEEHLVVRVKSSDVPPSMEHHEAGGSPSQQQMKPVISV
  TSALKEVGLDGSLTESQETSEEMEKSNNAISSKREILRRLNENLKAQEDEKEKHHHADSCETVGHRDERE
  YEKENAISSDRKKWEMGGQLVIPLDEVTLDASFSASEKHTVGEVIKLDSSGSPRKVWGKSPTDSVLKILG
  EAELQLQTELLENTPFKSEVCVEEENYKPLLTGEENLQCISKEVNPSATVDTETNSPKFAKVSPAMSEGN
  VEEPDDLETEVLQEPSAIPSDGSLPPVVNEVWTRAKATGLEDSIAVQQNDVCEERIPGTVDQSCKDHIEP
  AGNDSPQSGCDEEKSVQPELFFQKVVHSKDLNLVQSAHRSPEEAIPIRSHSDSPPKAKSKNSLLIGLSTG
  LFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVHQDSLEIEELEDEHIKEGPSDCEDIVFEETDTDLQE
  LQASMEQLLREQPGDEYSEEEESVLKSSNVERAARGTDVPDEEDNPSSESALNEEWHSDNSDAETTSECE
  YDSVFNHLEELRLHLEQEMGFEKFFEVYEKVKAIHEDEDENIEISSTIVENILGTEHQHLYAKILHLVMA
  DGAYQEDNDE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001106082
• ORF Size: 3600 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001106082.2, NP_001099552.2
• RefSeq Size: 4895
• RefSeq ORF: 3603
• Locus ID: 290705
• MW: 136.1 kDa