Gna15 (NM_053542) Rat Tagged ORF Clone

CAT#: RR214828

Gna15 (Myc-DDK-tagged ORF) - Rat guanine nucleotide binding protein, alpha 15 (Gna15), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gna15
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214828 representing NM_053542
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCGGTCCCTGACTTGGGGCTGCTGTCCCTGGTGCCTGACGGAAGAGGAGAAGACTGCCGCCAGAA
  TCGACCAGGAGATCAACAAGATTTTGTTGGAACAGAAGAAACAAGAGCGCGGGGAATTGAAACTCCTGCT
  GTTGGGGCCCGGTGAGAGCGGGAAAAGCACGTTCATCAAGCAAATGCGCATCATTCACGGCGCCGGCTAC
  TCTGAGGAGGACCGCAGAGCCTTCCGGCTGCTCGTCTACCAGAACATCTTCGTCTCCATGCAGGCCATGA
  TTGAAGCAATGGACAGGCTGCAGATCCCCTTCAGCAGGCCGGACAGCAAACAGCACGCCAGCCTGGTGAT
  GACCCAGGACCCCTATAAAGTGAGCTCGTTCGAGAAGCCATATGCAGTGGCCATGCAGTACCTGTGGCGG
  GACGCGGGCATCCGCGCATGCTACGAGCGGAGGCGTGAATTCCACCTGCTGGACTCCGCGGTGTACTACC
  TGTCACACCTGGAGCGCATCGCCGAGGACGACTACATCCCCACTGCGCAGGACGTGCTGCGCAGTCGCAT
  GCCCACCACTGGCATCAATGAGTACTGCTTTTCCGTGCAGAAAACCAAACTGCGCATCGTGGATGCTGGC
  GGCCAGAAGTCAGAACGTAAGAAATGGATCCACTGTTTCGAGAACGTGATTGCCCTCATCTACCTGGCGT
  CTCTGAGCGAGTATGACCAGTGTCTGGAGGAGAACAGTCAGGAGAACCGTATGAAGGAGAGTCTCGCTCT
  GTTTAGCACGATCCTAGAGCTGCCCTGGTTCAAGAGCACCTCGGTCATCCTCTTCCTCAACAAGACAGAC
  ATCCTGGAGGATAAAATCCACACCTCCCACCTAGCCTCATACTTCCCCAGCTTCCAGGGACCCCGGAGGG
  ACGCAGAGGCCGCCAAGCGCTTCATCTTGGACATGTACGCGCGCGTGTACGCGAGCTGTGCAGAGCCCCA
  CGACGGTGGCAGGAAGGGATCCCGCGCGCGCCGCCTCTTCGCACACTTCACCTGTGCCACGGACACGCAC
  AGCGTCCGCAGCGTGTTCAAGGACGTGCGGGACTCAGTGCTGGCCCGGTACCTGGACGAGATCAACCTGT
  TG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214828 representing NM_053542
  Red=Cloning site Green=Tags(s)
  
  MARSLTWGCCPWCLTEEEKTAARIDQEINKILLEQKKQERGELKLLLLGPGESGKSTFIKQMRIIHGAGY
  SEEDRRAFRLLVYQNIFVSMQAMIEAMDRLQIPFSRPDSKQHASLVMTQDPYKVSSFEKPYAVAMQYLWR
  DAGIRACYERRREFHLLDSAVYYLSHLERIAEDDYIPTAQDVLRSRMPTTGINEYCFSVQKTKLRIVDAG
  GQKSERKKWIHCFENVIALIYLASLSEYDQCLEENSQENRMKESLALFSTILELPWFKSTSVILFLNKTD
  ILEDKIHTSHLASYFPSFQGPRRDAEAAKRFILDMYARVYASCAEPHDGGRKGSRARRLFAHFTCATDTH
  SVRSVFKDVRDSVLARYLDEINLL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053542
• ORF Size: 1122 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_053542.1, NP_445994.1
• RefSeq Size: 1691
• RefSeq ORF: 1125
• Locus ID: 89788
• MW: 43.3 kDa
• Gene Summary: alpha subunit of an heterotrimeric G-protein; may function in taste signal transduction [RGD, Feb 2006]