Chrna2 (NM_133420) Rat Tagged ORF Clone

CAT#: RR214926

Chrna2 (Myc-DDK-tagged ORF) - Rat cholinergic receptor, nicotinic, alpha 2 (neuronal) (Chrna2), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Chrna2
• Synonyms: ACHR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214926 representing NM_133420
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCCTTTCCCATTCTGCTCTCCAGTTCTGGACACATCTTTATCTCTGGTGTCTCCTTCTGGTGCCAG
  CAGTGTTGACCCAGCAAGGCTCACACACCCATGCTGAGGACCGCCTGTTCAAACACCTGTTTGGAGGCTA
  CAATCGCTGGGCACGGCCAGTGCCCAACACTTCTGATGTGGTCATCGTGCGCTTTGGATTATCCATTGCT
  CAGCTCATAGATGTGGATGAGAAGAATCAAATGATGACCACCAATGTCTGGCTAAAGCAGGAATGGAATG
  ACTACAAGCTGCGCTGGGACCCGGCTGAGTTTGGCAATGTCACCTCCCTGCGCGTCCCTTCAGAGATGAT
  CTGGATCCCAGACATTGTCCTCTACAACAATGCAGATGGGGAGTTTGCGGTGACCCACATGACCAAGGCT
  CACCTCTTCTTCACGGGCACTGTGCACTGGGTGCCCCCAGCCATCTACAAGAGCTCCTGCAGCATCGATG
  TGACCTTCTTCCCCTTCGACCAGCAGAACTGCAAGATGAAGTTTGGCTCCTGGACATATGACAAGGCCAA
  GATCGATCTGGAGCAGATGGAGAGGACAGTGGACCTGAAGGACTACTGGGAGAGTGGCGAGTGGGCCATT
  ATCAATGCCACCGGAACCTATAACAGTAAGAAGTACGACTGCTGCGCGGAGATCTACCCCGATGTCACCT
  ACTACTTTGTGATCCGCCGGCTGCCGCTGTTCTATACCATCAACCTCATCATCCCATGCCTGCTCATCTC
  CTGCCTCACTGTGCTCGTGTTCTACCTGCCTTCCGAGTGTGGAGAGAAGATCACGCTGTGCATCTCGGTG
  CTGCTATCTCTCACTGTCTTCCTGCTGCTCATCACGGAGATCATCCCGTCCACCTCGCTGGTCATCCCAC
  TCATCGGCGAGTACCTGCTCTTCACCATGATCTTTGTCACCCTCTCTATCGTTATCACAGTCTTCGTGCT
  CAATGTACACCACCGCTCCCCCAGCACCCACAACATGCCCAACTGGGTAAGGGTAGCCCTGCTAGGCCGG
  GTGCCCAGGTGGCTGATGATGAACCGGCCCCTGCCACCTATGGAGCTCCATGGCTCCCCGGATCTGAAGC
  TCAGCCCCTCATACCATTGGCTAGAGACTAACATGGATGCTGGAGAAAGGGAGGAGACAGAGGAAGAGGA
  GGAAGAAGAAGATGAAAACATATGTGTGTGTGCAGGCCTTCCAGACTCTTCGATGGGTGTCCTCTATGGC
  CATGGCGGCCTGCATCTGAGAGCCATGGAGCCTGAGACCAAGACTCCATCCCAGGCTAGCGAGATTCTGC
  TGTCACCTCAAATACAGAAAGCACTAGAAGGTGTACACTACATTGCTGACCGTCTGAGGTCTGAGGATGC
  TGACTCTTCGGTGAAGGAAGACTGGAAGTATGTGGCCATGGTGGTAGACCGGATATTCCTCTGGCTGTTC
  ATTATCGTCTGCTTCCTGGGGACCATCGGACTCTTCCTTCCTCCATTCCTGGCTGGAATGATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214926 representing NM_133420
  Red=Cloning site Green=Tags(s)
  
  MTLSHSALQFWTHLYLWCLLLVPAVLTQQGSHTHAEDRLFKHLFGGYNRWARPVPNTSDVVIVRFGLSIA
  QLIDVDEKNQMMTTNVWLKQEWNDYKLRWDPAEFGNVTSLRVPSEMIWIPDIVLYNNADGEFAVTHMTKA
  HLFFTGTVHWVPPAIYKSSCSIDVTFFPFDQQNCKMKFGSWTYDKAKIDLEQMERTVDLKDYWESGEWAI
  INATGTYNSKKYDCCAEIYPDVTYYFVIRRLPLFYTINLIIPCLLISCLTVLVFYLPSECGEKITLCISV
  LLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHHRSPSTHNMPNWVRVALLGR
  VPRWLMMNRPLPPMELHGSPDLKLSPSYHWLETNMDAGEREETEEEEEEEDENICVCAGLPDSSMGVLYG
  HGGLHLRAMEPETKTPSQASEILLSPQIQKALEGVHYIADRLRSEDADSSVKEDWKYVAMVVDRIFLWLF
  IIVCFLGTIGLFLPPFLAGMI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133420
• ORF Size: 1533 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_133420.1, NP_596911.1
• RefSeq Size: 1931
• RefSeq ORF: 1536
• Locus ID: 170945
• MW: 58.6 kDa
• Gene Summary: agonist-binding subunit of neuronal nicotinic acetylcholine receptor [RGD, Feb 2006]