Eme1 (NM_001105830) Rat Tagged ORF Clone

CAT#: RR215007

Eme1 (Myc-DDK-tagged ORF) - Rat essential meiotic endonuclease 1 homolog 1 (S. pombe) (Eme1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Eme1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR215007 representing NM_001105830
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCTAAGAAGGTTATCCCTCTCTCTGCTGTCCACGGAGAGTGACTCTGAGGAACTGCCCACATTCG
  CCTTTCTGAAGAAGGAACCAGCTTCAACGGACAGCAATCCACCTCAGAGAGAGAAGAACATTGTTGTTGT
  CACCTCAGATATTGAGGCCTCCTGTCCTCCATCACCAGGCTTAAAAGACCCACCATGTGTCCCCAGTGCA
  GCGGAAGCTCCCACACAAGCAGGGCCAGTCAGAGTGCTGAGCAGTAGCAGTGAGGATGAAGATGGATTGG
  TCTCTCTGGCTGAAAGGATCACATGGAAGCTTTTGAGGAACAAACAGCTGAGCCCTGAGCGCTCTCGCTC
  CCCACTTAAAACGGGTTCAGATCACCAAAAAAATGCAAGTACACCATGTGACTGGAAAAGGCAACCGTGT
  CCAAAGATTCCTGATGTTCCTCTCCACAGTGCCCTAGAGAAGAGTCCAGCAAATGACAAAGACTCCCTCT
  TAGACAATCAGTGCCATCAACTTCCTGCCTACCAGGCTACAGGCAGGGAGTTGGCAGTCACCAAAACAAA
  TCCTGACCTCCCTCTACCCAAGAAGAGAACCAAGCACATTCAGACAGCCCAGAGCAGAGGCCCTCAGGGA
  TGCTGGCAGCCAAGACAGGCAAGCAGGAAGGAAAACACCCCAAGGCAGCAGGAAAGGAAAAAGAAGGCAG
  AGATGGTCAACAGGCTCAGAGCCCAGAGGCCAGAGGAATGCCTGAAACACATCGTGGTGGTGCTGGATCC
  AGTCCTTTTACAGATGGAAGGTGGGGGCCAGCTCCTGGGAGCGCTGCAGGCCATGGAGTGCAGCTGTGTG
  ATTGAAGCTCAGGTCGTACCTCAAAGTATCACTTGGAGGAGGAAGACAGAGCTGGAAGAGGATGGAGATG
  ACTGGGTGGAAGAGCCAACAATCCTGGTGTTGGTCCTCAAAGAGGTCTTCATGTCCATGGTCTACAACTC
  AAAGCAGGGAAATCCTGGCAGCACTGAGAAAGGGAAGGAGACACTTCGGGGCTTTGTAACTGATGTCACA
  GCAAGGACAGGGAAAGCATTGTCACTGGTGATTGTGGACCAGGAGAAATGCCTCAGGGCTCAGAATCCCC
  CGAAAAGAAGGAAATCAGGAATGGCAAATAAAAAGGCCAAAGAGAAGCATCAGCAGAGACGAGAGTCCAG
  CACAAGGCTCATGGTGTCCAGGGTAGACATGGAAGAGGCATTGGTGGATCTGCAGCTATATACAGAAGCC
  CAGGCTCGGATCGTGCAGAGCTGGAGAGAGCTGGCCGACTTCGCCTGTGCATTTACAAAGGCTGTGGCTG
  AAGCGCCCTTCAAGAAACTCCGGGATCAAGGCAGCTTCTCCTTCTTCCTGGAGAAAGACTGGGCTGGAGG
  GGTGAAAGCGGACCATGCTGGTAGAGGACTTGCACTGATCTGGAGGCGGCAGATTCAGCAGCTGAACCGA
  GTCAGCTCAGAAATGGCCGGCGCTATTGTGGATGCCTATCCTTCTCCACAGCTCCTGGTGCAGGCTTATC
  AGCAGTGTTTTTCTGAACAAGAACGCCAGAATTTGCTGGCTAACATACAGGTGCGACGTGGGGAAGGTGT
  GACAGCCACCTCCCGCCGTGTTGGGCCAGAATTATCCAGGCGGATCTACCTTCAAATGACCACAGTGCAA
  CCAGATCTCATCTTAGACAGTGTTGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR215007 representing NM_001105830
  Red=Cloning site Green=Tags(s)
  
  MALRRLSLSLLSTESDSEELPTFAFLKKEPASTDSNPPQREKNIVVVTSDIEASCPPSPGLKDPPCVPSA
  AEAPTQAGPVRVLSSSSEDEDGLVSLAERITWKLLRNKQLSPERSRSPLKTGSDHQKNASTPCDWKRQPC
  PKIPDVPLHSALEKSPANDKDSLLDNQCHQLPAYQATGRELAVTKTNPDLPLPKKRTKHIQTAQSRGPQG
  CWQPRQASRKENTPRQQERKKKAEMVNRLRAQRPEECLKHIVVVLDPVLLQMEGGGQLLGALQAMECSCV
  IEAQVVPQSITWRRKTELEEDGDDWVEEPTILVLVLKEVFMSMVYNSKQGNPGSTEKGKETLRGFVTDVT
  ARTGKALSLVIVDQEKCLRAQNPPKRRKSGMANKKAKEKHQQRRESSTRLMVSRVDMEEALVDLQLYTEA
  QARIVQSWRELADFACAFTKAVAEAPFKKLRDQGSFSFFLEKDWAGGVKADHAGRGLALIWRRQIQQLNR
  VSSEMAGAIVDAYPSPQLLVQAYQQCFSEQERQNLLANIQVRRGEGVTATSRRVGPELSRRIYLQMTTVQ
  PDLILDSVD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001105830
• ORF Size: 1707 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001105830.2, NP_001099300.2
• RefSeq Size: 1710
• RefSeq ORF: 1710
• Locus ID: 287634
• MW: 63.6 kDa