Notch4 (NM_001002827) Rat Tagged ORF Clone

CAT#: RR215069

Notch4 (Myc-DDK-tagged ORF) - Rat Notch homolog 4 (Drosophila) (Notch4), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Notch4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR215069 representing NM_001002827
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCGCCAGTGGCTGCTGCTTCTGCTCTTGCCGCTCAGCTTCCCAGTCATCCTGACCAGAGAGCTTC
  TGTGTGGAGGATCCCCAGAGCCCTGTGCCAACGGAGGCACCTGCCTGAGGCTATCTCAGGGACAAGGGAC
  CTGCCAGTGTGCCCCTGGATTTCTGGGTGAGACTTGCCAGTTCCCTGACCCCTGCTGGGACACCCAACTC
  TGCGAGAACGGTGGCAGCTGTCAAGCCTTGCTCCCCACAGCCCCAAGCTCCCACAGCCCCACTTCTCCAC
  TGACCCCCCACTTCTCCTGCACCTGCCCCTCTGGCTTCACTGGTGATCGATGCCAAAGCCCTCTGGAAGA
  GCTCTGTCCGCCTTCTTTCTGTTCCAACGGGGGTCACTGCTCTGTCCAGGTCTCGGGCCGCCCACAGTGT
  TCCTGCGAGCCCGGGTGGACAGGTGAGCAGTGCCAGCTCCGAGACTTCTGCTCAGCCAACCCCTGTGCCA
  ACGGAGGTGTGTGCTTGGCCACATACCCCCAGATCCAGTGTCGTTGTCCAACCGGCTTCGAGGGTCACAT
  CTGTGAACGCGACGTCAATGAATGCTTCCTGGAGCCCGGACCCTGCCCTCGGGGCACCTCCTGTCACAAC
  ACCTTGGGCTCCTTCCAGTGCCTCTGCCCTGTGGGGCAGGAAGGTCCCCAGTGCAAGCTCAGGAAGGGAG
  CCTGCCTTCCTGGAACCTGTCTCAATGGAGGCACCTGCCAGCTGGTGCCAGAGGGAGACACTACCTTTCA
  TCTCTGCCTCTGTCCCCCAGGTTTCACAGGGCTGAACTGTGAGATGAATCCAGATGACTGTGTCAGGAAC
  CAGTGTCAGAACGGGGCCACCTGCCAGGATGGGCTGGGCACCTACACCTGCCTCTGCCCCAAGACGTGGA
  AGGGCTGGGACTGCTCCGAAGACATAGATGAATGTGAAGCCCAGGGTCCGCCTCGATGCAGAAACGGTGG
  CACCTGCCAGAACTCGGCTGGTGGCTTTCACTGTGTGTGTGTGAGTGGCTGGGGAGGCGAGGGCTGTGAC
  GAGAACCTGGATGACTGTGCGGCCGCCACCTGTGCCCTGGGATCCACCTGCATCGACCGTGTGGGCTCCT
  TCTCCTGCCTCTGCCCACCTGGACGCACAGGCCTCCTGTGCCACCTGGAAGACATGTGTTTGAGGCAGCC
  GTGCCATGTGAATGCCCAGTGCAGCACCAACCCCCTGACGGGGTCTACCCTCTGCATATGCCAGCCGGGC
  TACTCGGGACCCACCTGCCACCAAGATCTGGATGAGTGTCAAATGGCCCAGCAAGGACCCAGTCCCTGTG
  AACACGGCGGCTCCTGCATCAACACCCCTGGCTCCTTCAACTGCCTCTGCCTGCCTGGCTACACGGGCTC
  CCGCTGCGAGGCTGACCACAATGAGTGCCTGTCGCAGCCCTGTCACCCGGGCAGCACCTGCCTGGACCTG
  CTTGCCACCTTCCAGTGCCTCTGCCCACCAGGCCTGGAAGGGAGGCTCTGTGAAGTGGAGATCAATGAGT
  GTGCCTCTAACCCCTGCCTCAACCAAGCTGCCTGTCACGACCAGCTCAACGGCTTCCTGTGCCTCTGCCT
  TCCTGGATTCACTGGTGCCAGATGCGAGAAAGACATGGACGAGTGCAGCAGCGCCCCCTGTGCCAATGGG
  GGGCACTGCCAAGACCAGCCTGGAGCCTTCCACTGCGAGTGTCTCCCAGGCTTTGAAGGGCCACGCTGTG
  AGACTGAAGCGGATGAATGTCGGAGTGACCCCTGCCCCGTGGGAGCCAGCTGCCTTGATCTTCCCGGGGC
  GTTCCTCTGCCTCTGCCGGCCTGGTTTCACAGGTCAACTTTGTGAGGTTCCCTTGTGTAGCCCCATCCTG
  TGCCAACCTGGACAGCAATGCCAAGACCAGGAACACAGAGCCCCCTGCCTCTGCCCTGACGGAAGTCCTG
  GCTGTGTTCCTGCTGAGGACGACTGCCCCTGCCACCATGGCCATTGCCAGAGATCCTTGTGTGTGTGTAA
  CGAGGGCTGGACTGGACCAGAGTGTGAGACAGAGCTGGGCGGCTGCCTCTCCACACCCTGTGCCCATGGG
  GGGACCTGCCACCCACAGCCCTCTGGCTACAACTGTAGCTGCCTTGCAGGCTACACGGGGCTGACCTGTA
  GTGAGGAGATAACAGCTTGTCACTCAGGGCCCTGTCTCAATGGTGGCTCCTGCAGCATCCATCCTGAGGG
  CTACTCTTGCACCTGCCCTCCAAGTCACACAGGTCCCCACTGCCAGACTGCCGTGGACCACTGTGCGTCT
  GCCTCGTGTCTCAACGGGGGTACCTGCATGAGCAAACCTGGCACTTTCTTCTGCCACTGTGCCACTGGCT
  TCCAGGGGCTGCACTGTGAGAAGAAGATTCACCCCAGCTGTGCAGACAACCCCTGCAGGAACAAGGCAAC
  CTGCCAAGACACACCTCGAGGGGCCCGCTGCCTCTGCAGCCCTGGCTACACAGGAAGCAGCTGCCAGACT
  CTGATAGACTTGTGTGCCCGGAAGCCCTGTCCACACACTGCCCGATGCCTCCAGAGTGGGCCCTCCTTCC
  ACTGCCTGTGCCACCAGGGATGGACAGGGTCTCTCTGTGACCTCCCACTGTCCTGCCAGGCGGCCGCGAT
  GAGCCAAGGTGTAGAGATCTCCAACCTGTGCCAGAATGGGGGCCTCTGTATTGACACGGGCTCCTCCTAT
  TTCTGTCGCTGCCCTCCTGGATTCGAGGGCAAGTTATGCCAGGATACTGTGAACCCCTGCACGTCCAAGC
  CTTGCCTTCACGGGGCTACCTGTGTGCCCCAGCCCAACGGCTATGTCTGCCAGTGTGCCCCAGGCTATGA
  AGGACAGAACTGCTCAAAAGTACACGACGCTTGCCAGTCCGGGCCGTGCCACAACCATGGAACCTGTACC
  CCCAGGCCTGGAGGCTTCCACTGTGCCTGCCCTCCAGGTTTTGTGGGGCTGCGCTGTGAGGGGGATGTGG
  ACGAGTGTCTCGACCGGCCCTGCCACCCCTCGGGCACTGCGTCCTGCCATTCTTTAGCCAACGCCTTCTA
  TTGCCAGTGTCTGCCTGGGCACACAGGCCAGCGGTGTGAAGTGGAGATGGACCTCTGCCAGAGCCAGCCC
  TGCTCCAATGGAGGGTCCTGTGAGGTCACAACAGGACCGCCCCCTGGCTTCACCTGCCGCTGCCCCGAGG
  GTTTTGAAGGCCCCACCTGCAGCCGCAAAGCCCCTGCCTGCGGCAACCATCATTGCCACAACGGAGGCCT
  ATGTCTGCCCTCCCCTAAGCCAGGCTCCCCACCCCTCTGTGCCTGCCTCAGTGGCTTCGGGGGCCCTGAC
  TGTCTGACACCTCCAGCTCCACCAGGGTGCGGTCCTCCCTCCCCCTGCATGCACAATGGTAGCTGCACTG
  AGACCCCCGGGTTGGGCAATCCTGGCTTTCAATGCACCTGCCCTCCTGACTCTCCGGGGCCCCGGTGTCA
  AAGGCCAGGGGCGAATGGATGTGAGGGCCGAGGTGGCGATGGAGCCTGTGATGCTGGCTGCAGTGGCCCA
  GGAGGGGACTGGGATGGAGGGGACTGTTCCCTGGGGGTCCCAGACCCCTGGAAGGGCTGTCCCCCGCATT
  CCCAGTGCTGGCTTCTCTTCCGGGATGGGCGGTGCCACCCACAGTGCGACTCTGAGGAGTGTCTGTTTGA
  TGGCTATGACTGTGAGATCCCTCCAACTTGCACCCCAGCCTACGACCAGTACTGCCGAGATCACTTCCAC
  AACGGGCACTGTGAGAAAGGCTGCAACAACGCTCAGTGTGGCTGGGATGGGGGTGACTGCAGGCCAGAAG
  GGGATGACTCAGAGGGGGGCCCCTCCCTGGCCCTGCTGGTGGTGCTGAGTCCCCCAGCCTTGGATCAGCA
  GCTGCTTGCCCTGGCTCGAGTACTGTCCCTGACTCTGAGGGTCGGTCTCTGGGTGAGGAAGGACAGTGAA
  GGCAGGAACATGGTGTTCCCCTATCCTGGGACCCGGGCCAAAGAGGAGCTGAGTGGAACTAGAGATTCCT
  CTTCGTGGGAAAGACAAGCCCCTCACACTCAGACCCCGGGCAAAGAGACAGAGTCCCTTGGTGCAGGGTT
  TGTGGTAGTGATGGGAGTGGATTTGTCCCGCTGTGGTCCAGAGCATCCTGCGTCCCGCTGTCCCCGGGAC
  TCTGGGCTCCTGCTGCGTTTCCTTGCAGCAATGGCCGCAGTGGGAGCTCTGGAGCCCCTGCTGCCTGGAC
  CCTTGCTGGCTGCTCACCCTCAAGCAGGGACCGGGTCCCCCGCCACCCGGCTTCCCTGGCCCATTCTGTG
  TTCGCCAGTGGTCGGGGTGCTCCTCCTGGCCCTTGGGGCTCTTCTCGTCCTCCAGCTCATCCGGCGCCGG
  CGGCGGGAACACGGGGCCCTGTGGCTGCCCCCTGGTTTCATTCGAAGGCCACAGACACAGCAGGCACCCC
  ACCGGCGGAGGCCCCCACTGGGCGAGGACAACATTGGTCTCAAGGCGCTGAAGCCAGAGGCAGAAGTGGA
  TGAGGACGGAGTGGTCATGTGCTCGGGCCCTGAAGAGGGCGAGGCAGAAAAAACAGCCTCAGCCTCCAGG
  TGCCAGCTCTGGCCACTGCGCAGCGGCTGTGAAGGGCTCCCACAGGCAGTCATGCTGACCCCTCCTCAGG
  AGTGTGAATCAGAACTTCCAGATTCGGACACCTGTGGACCTGATGGGGTGACACCCCTGATGTCTGCCGT
  CTTCTGTGGGGGAGTGCAGTCCACCACTGTACAGAGACTGGGACTAGGAAATCCCGAACCCTGGGAACCA
  CTGCTGGATAGAGGGGCCTGCCCCCAGGCTCACACTGTGGGCACTGGAGAGACGCCCCTGCACCTAGCTG
  CCCGATTCTCTCGGCCAACTGCTGCCCGACGCCTCCTGGAGGCTGGAGCCAACCCCAACCAGCCAGACCG
  GGCTGGGCGTACCCCACTTCACACTGCAGTAGCTGCTGATGCTCGGGAGGTTTGCCAGCTCCTATTGGCC
  AGCAGACAGACCGCTGTAGACGCGCGCACAGACGACGGGACTACACCTTTGATGCTGGCTGCCAGGTTGG
  CCGTGGAGGACCTGGTTGAAGAATTGATCGCAGCCCGAGCAGATGTAGGAGCCAGAGATAAAAGGGGAAA
  AACTGCACTGCACTGGGCCGCTGCAGTGAACAACGCCCGAGCTGCCCGCTGTCTCCTCCAGACTGGAGCG
  GATAAAGATGCCCAGGACAGTAGGGAGCAGACGCCGCTGTTCCTGGCTGCGCGCGAAGGCGCCGTGGAGG
  TGGCACAGCTGCTGCTGGAGATCGGGGCAGCGCGGGGGCTGCGGGACCAGGCCGGGCTGGCCCCAGCAGA
  TGTGGCCCGCCAGCGTAGTCATTGGGACCTGCTGACGCTGCTGGAGGGGGCAGGACCGATCACGCAGGAG
  GCCCGTTCGCACGCACGCAACACGCCGGGGGGCGGGGCCGCGCCGCGCTGCCGGACGCTGTCTGCCGGAG
  CGCGCCCGCGCGGGGGCGGAGCTTGTCTGCAAGCTCGCACTTGGTCGGTGGACTTGGGAGCGCGCCGAGG
  GGCGGTATACGCGCGCTGCCGGAGCCGATCAGGAGGCTCTGGAGGCCCCTCCATGCGCGGCCGCAGGCTT
  TCCGCGGACTCCCGTGGACGACGCGGGGCTAGAGTATCACAGGATGACTGGCCTCGGGACTGGGTGGCCC
  TGGAAGCCTGCGGCTCTGCCTGCAGTGCGCCGATCCCGCCTCCCAGCCTTACCCCGTCCCCAGAACGCGG
  ATCCCCTCAAGTTTCCTGGGGTCTTCCAGTCCACCAAGAGGTCCCCTTAAACTCGGGTGGAAGGAATCAG
  AAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR215069 representing NM_001002827
  Red=Cloning site Green=Tags(s)
  
  MQRQWLLLLLLPLSFPVILTRELLCGGSPEPCANGGTCLRLSQGQGTCQCAPGFLGETCQFPDPCWDTQL
  CENGGSCQALLPTAPSSHSPTSPLTPHFSCTCPSGFTGDRCQSPLEELCPPSFCSNGGHCSVQVSGRPQC
  SCEPGWTGEQCQLRDFCSANPCANGGVCLATYPQIQCRCPTGFEGHICERDVNECFLEPGPCPRGTSCHN
  TLGSFQCLCPVGQEGPQCKLRKGACLPGTCLNGGTCQLVPEGDTTFHLCLCPPGFTGLNCEMNPDDCVRN
  QCQNGATCQDGLGTYTCLCPKTWKGWDCSEDIDECEAQGPPRCRNGGTCQNSAGGFHCVCVSGWGGEGCD
  ENLDDCAAATCALGSTCIDRVGSFSCLCPPGRTGLLCHLEDMCLRQPCHVNAQCSTNPLTGSTLCICQPG
  YSGPTCHQDLDECQMAQQGPSPCEHGGSCINTPGSFNCLCLPGYTGSRCEADHNECLSQPCHPGSTCLDL
  LATFQCLCPPGLEGRLCEVEINECASNPCLNQAACHDQLNGFLCLCLPGFTGARCEKDMDECSSAPCANG
  GHCQDQPGAFHCECLPGFEGPRCETEADECRSDPCPVGASCLDLPGAFLCLCRPGFTGQLCEVPLCSPIL
  CQPGQQCQDQEHRAPCLCPDGSPGCVPAEDDCPCHHGHCQRSLCVCNEGWTGPECETELGGCLSTPCAHG
  GTCHPQPSGYNCSCLAGYTGLTCSEEITACHSGPCLNGGSCSIHPEGYSCTCPPSHTGPHCQTAVDHCAS
  ASCLNGGTCMSKPGTFFCHCATGFQGLHCEKKIHPSCADNPCRNKATCQDTPRGARCLCSPGYTGSSCQT
  LIDLCARKPCPHTARCLQSGPSFHCLCHQGWTGSLCDLPLSCQAAAMSQGVEISNLCQNGGLCIDTGSSY
  FCRCPPGFEGKLCQDTVNPCTSKPCLHGATCVPQPNGYVCQCAPGYEGQNCSKVHDACQSGPCHNHGTCT
  PRPGGFHCACPPGFVGLRCEGDVDECLDRPCHPSGTASCHSLANAFYCQCLPGHTGQRCEVEMDLCQSQP
  CSNGGSCEVTTGPPPGFTCRCPEGFEGPTCSRKAPACGNHHCHNGGLCLPSPKPGSPPLCACLSGFGGPD
  CLTPPAPPGCGPPSPCMHNGSCTETPGLGNPGFQCTCPPDSPGPRCQRPGANGCEGRGGDGACDAGCSGP
  GGDWDGGDCSLGVPDPWKGCPPHSQCWLLFRDGRCHPQCDSEECLFDGYDCEIPPTCTPAYDQYCRDHFH
  NGHCEKGCNNAQCGWDGGDCRPEGDDSEGGPSLALLVVLSPPALDQQLLALARVLSLTLRVGLWVRKDSE
  GRNMVFPYPGTRAKEELSGTRDSSSWERQAPHTQTPGKETESLGAGFVVVMGVDLSRCGPEHPASRCPRD
  SGLLLRFLAAMAAVGALEPLLPGPLLAAHPQAGTGSPATRLPWPILCSPVVGVLLLALGALLVLQLIRRR
  RREHGALWLPPGFIRRPQTQQAPHRRRPPLGEDNIGLKALKPEAEVDEDGVVMCSGPEEGEAEKTASASR
  CQLWPLRSGCEGLPQAVMLTPPQECESELPDSDTCGPDGVTPLMSAVFCGGVQSTTVQRLGLGNPEPWEP
  LLDRGACPQAHTVGTGETPLHLAARFSRPTAARRLLEAGANPNQPDRAGRTPLHTAVAADAREVCQLLLA
  SRQTAVDARTDDGTTPLMLAARLAVEDLVEELIAARADVGARDKRGKTALHWAAAVNNARAARCLLQTGA
  DKDAQDSREQTPLFLAAREGAVEVAQLLLEIGAARGLRDQAGLAPADVARQRSHWDLLTLLEGAGPITQE
  ARSHARNTPGGGAAPRCRTLSAGARPRGGGACLQARTWSVDLGARRGAVYARCRSRSGGSGGPSMRGRRL
  SADSRGRRGARVSQDDWPRDWVALEACGSACSAPIPPPSLTPSPERGSPQVSWGLPVHQEVPLNSGGRNQ
  N
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001002827
• ORF Size: 5883 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001002827.1, NP_001002827.1
• RefSeq Size: 6483 bp
• RefSeq ORF: 5886 bp
• Locus ID: 406162
• Cytogenetics: 20p12
• MW: 206.1 kDa