Hspa1a (NM_031971) Rat Tagged ORF Clone

CAT#: RR215127

Hspa1a (Myc-DDK-tagged ORF) - Rat heat shock 70kD protein 1A (Hspa1a), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hspa1a
• Synonyms: Hsp70-1; Hsp70-2; HSP70.1; HSP70.2; HSP72; Hspa1; Hspa1b; Hspa2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR215127 representing NM_031971
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAAGAAAACAGCGATCGGCATCGACCTGGGCACCACCTACTCGTGCGTGGGCGTGTTCCAGCACG
  GCAAGGTGGAGATCATCGCCAACGACCAGGGCAACCGCACGACCCCCAGCTACGTGGCCTTCACCGACAC
  CGAGCGGCTCATCGGGGACGCCGCCAAGAACCAGGTGGCGCTGAACCCGCAGAACACCGTGTTCGACGCG
  AAGCGGCTGATCGGCCGCAAGTTCGGCGACCCGGTGGTGCAGTCGGACATGAAGCACTGGCCCTTCCAGG
  TGGTGAACGACGGCGACAAGCCCAAGGTGCAGGTGAACTACAAGGGCGAGAACCGGTCGTTCTACCCGGA
  GGAGATCTCGTCCATGGTGCTGACCAAGATGAAGGAGATCGCCGAGGCGTACCTGGGCCACCCGGTGACC
  AACGCGGTGATCACCGTGCCCGCCTACTTCAACGACTCGCAGCGGCAGGCCACCAAGGACGCGGGCGTGA
  TCGCGGGTCTGAACGTGCTGCGGATCATCAACGAGCCCACGGCGGCCGCCATCGCCTACGGGCTGGACCG
  GACCGGCAAGGGCGAGCGCAACGTGCTCATCTTCGACCTGGGGGGCGGCACGTTCGACGTGTCCATCCTG
  ACGATCGACGACGGCATCTTCGAGGTGAAGGCCACGGCGGGCGACACGCACCTGGGCGGGGAGGACTTCG
  ACAACCGGCTGGTGAGCCACTTCGTGGAGGAGTTCAAGAGGAAGCACAAGAAGGACATCAGCCAGAACAA
  GCGCGCGGTGCGGCGACTGCGCACGGCGTGCGAGAGGGCCAAGAGGACGCTGTCGTCCAGCACCCAGGCC
  AGCCTGGAGATCGACTCTCTGTTCGAGGGCATCGACTTCTACACGTCCATCACGCGGGCGCGGTTCGAGG
  AGCTGTGCTCGGACCTGTTCCGCGGCACGCTGGAGCCCGTGGAGAAGGCCCTGCGCGACGCCAAGCTGGA
  CAAGGCGCAGATCCACGACCTGGTGCTGGTGGGCGGCTCGACGCGCATCCCCAAGGTGCAGAAGCTGCTG
  CAGGACTTCTTCAACGGGCGCGACCTGAACAAGAGCATCAATCCGGACGAGGCGGTGGCCTACGGGGCGG
  CGGTGCAGGCGGCCATCCTGATGGGGGACAAGTCGGAGAACGTGCAGGACCTGCTGCTGCTGGACGTGGC
  GCCGCTGTCGCTGGGTCTGGAGACCGCGGGCGGCGTGATGACGGCGCTCATCAAGCGCAACTCCACCATC
  CCCACCAAGCAGACGCAGACCTTCACCACCTACTCGGACAACCAGCCCGGGGTGCTGATCCAGGTGTACG
  AGGGCGAGAGGGCCATGACGCGCGACAACAACCTGCTGGGGCGCTTCGAGTTGAGCGGCATCCCGCCGGC
  TCCCAGGGGCGTGCCCCAGATCGAGGTGACCTTCGACATCGACGCCAACGGCATCCTGAACGTCACGGCC
  ACTGACAAGAGCACCGGCAAGGCCAACAAGATCACCATCACCAACGACAAGGGCCGCCTGAGCAAGGAGG
  AGATCGAGCGCATGGTGCAGGAGGCCGAGCGCTACAAGGCGGAGGACGAGGTGCAGCGCGAGAGGGTGGC
  TGCCAAGAATGCGCTCGAGTCCTATGCCTTCAACATGAAGAGCGCCGTGGAGGACGAGGGTCTCAAGGGC
  AAGATCAGCGAGGCTGACAAGAAGAAGGTGCTGGACAAGTGCCAGGAGGTCATCTCCTGGCTGGACTCTA
  ACACGCTGGCTGAGAAAGAGGAGTTCGTGCACAAGCGGGAGGAGCTGGAGCGGGTGTGCAACCCGATCAT
  CAGCGGGCTGTATCAGGGTGCGGGTGCTCCCGGGGCTGGGGGCTTCGGGGCCCAGGCGCCCAAGGGAGGC
  TCTGGGTCGGGGCCCACCATCGAGGAGGTGGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR215127 representing NM_031971
  Red=Cloning site Green=Tags(s)
  
  MAKKTAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVALNPQNTVFDA
  KRLIGRKFGDPVVQSDMKHWPFQVVNDGDKPKVQVNYKGENRSFYPEEISSMVLTKMKEIAEAYLGHPVT
  NAVITVPAYFNDSQRQATKDAGVIAGLNVLRIINEPTAAAIAYGLDRTGKGERNVLIFDLGGGTFDVSIL
  TIDDGIFEVKATAGDTHLGGEDFDNRLVSHFVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSTQA
  SLEIDSLFEGIDFYTSITRARFEELCSDLFRGTLEPVEKALRDAKLDKAQIHDLVLVGGSTRIPKVQKLL
  QDFFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSENVQDLLLLDVAPLSLGLETAGGVMTALIKRNSTI
  PTKQTQTFTTYSDNQPGVLIQVYEGERAMTRDNNLLGRFELSGIPPAPRGVPQIEVTFDIDANGILNVTA
  TDKSTGKANKITITNDKGRLSKEEIERMVQEAERYKAEDEVQRERVAAKNALESYAFNMKSAVEDEGLKG
  KISEADKKKVLDKCQEVISWLDSNTLAEKEEFVHKREELERVCNPIISGLYQGAGAPGAGGFGAQAPKGG
  SGSGPTIEEVD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031971
• ORF Size: 1923 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_031971.1, NM_031971.2, NP_114177.2
• RefSeq Size: 4045
• RefSeq ORF: 1926
• Locus ID: 24472
• MW: 70.2 kDa
• Gene Summary: heat inducible gene; induced by global ischemia and kainic acid-induced seizures [RGD, Feb 2006]