Dhcr7 (NM_022389) Rat Tagged ORF Clone

CAT#: RR215142

Dhcr7 (Myc-DDK-tagged ORF) - Rat 7-dehydrocholesterol reductase (Dhcr7), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dhcr7
• Synonyms: MGC93039
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR215142 representing NM_022389
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCAAAGTCCCAGCACAACGCTTCCAAAGCCAAGAATCACAACGTCAAGGCTGAATCTCAAGGAC
  AATGGGGCCGTGCCTGGGAAGTGGACTGGTTCTCCCTGGTCAGCGTCATTTTCCTGCTGCTCTTCGCACC
  GTTCATTGTGTACTACTTCATCATGGCATGTGACCAGTACAGCTGCTCGCTGACTGCCCCCATATTGGAC
  GTAGCTACAGGCCGTGCGAGTCTGGCAGACATCTGGGCCAAGACACCGCCTGTGACAGCTAAGGCTGCCC
  AACTGTATGCCTTGTGGGTCAGCTTCCAGGTGCTGCTTTACTCCTGGCTTCCTGACTTCTGCCACAGATT
  TCTGCCAGGTTATGTGGGAGGCGTCCAAGAAGGTGCCATAACTCCAGCAGGGATTGTAAATAAGTATGAG
  GTGAATGGGCTGCAAGCCTGGCTCATTACCCACTTCCTCTGGTTTGTGAACGCTTACCTCCTATCCTGGT
  TCTCCCCCACCATCATCTTTGACAACTGGATCCCGTTGCTATGGTGTGCCAACATCCTGGGCTACGCTGT
  GTCCACCTTTGCAATGATCAAGGGGTACCTGTTCCCCACCAGCGCCGAAGACTGCAAATTCACAGGCAAT
  TTCTTCTACAACTACATGATGGGGATTGAGTTCAACCCCCGCATCGGAAAGTGGTTTGACTTCAAGCTGT
  TCTTCAATGGGCGCCCGGGGATTGTGGCCTGGACTCTTATCAACCTGTCCTTTGCTGCCAAGCAGCAGGA
  GCTTTATGGCCATGTGACCAACTCCATGATTTTGGTCAATGTCTTACAGGCCATCTATGTGTTAGACTTC
  TTCTGGAACGAAACCTGGTACCTGAAGACCATTGACATCTGCCATGACCACTTTGGATGGTACCTGGGCT
  GGGGAGATTGCGTGTGGCTGCCCTACCTCTACACACTGCAGGGCCTGTACTTGGTGTACCACCCTGTACA
  GCTCTCCACCCCCAACGCCTTGGGCGTCCTGCTGCTCGGCCTGGTGGGTTACTATATCTTCCGAATGACC
  AACCATCAGAAGGACCTGTTCCGCCGGACAGATGGACACTGCCTCATCTGGGGCAAGAAACCCAAGGCCA
  TCGAGTGCTCCTACACGTCAGCCGACGGGCTGAAGCACCGCAGCAAGCTGTTGGTTTCAGGCTTCTGGGG
  TGTGGCCCGCCACTTCAACTATACCGGAGACCTGATGGGCAGCCTCGCCTACTGCCTGGCTTGTGGCGGC
  GGCCACCTCCTCCCCTACTTCTACATCATCTATATGACTATCCTGCTTACCCACCGCTGCCTCCGTGATG
  AGCACCGCTGTGCCAACAAGTATGGCCGTGACTGGGAGCGCTACGTAGCCGCTGTGCCCTACCGCCTGCT
  GCCTGGGATCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR215142 representing NM_022389
  Red=Cloning site Green=Tags(s)
  
  MASKSQHNASKAKNHNVKAESQGQWGRAWEVDWFSLVSVIFLLLFAPFIVYYFIMACDQYSCSLTAPILD
  VATGRASLADIWAKTPPVTAKAAQLYALWVSFQVLLYSWLPDFCHRFLPGYVGGVQEGAITPAGIVNKYE
  VNGLQAWLITHFLWFVNAYLLSWFSPTIIFDNWIPLLWCANILGYAVSTFAMIKGYLFPTSAEDCKFTGN
  FFYNYMMGIEFNPRIGKWFDFKLFFNGRPGIVAWTLINLSFAAKQQELYGHVTNSMILVNVLQAIYVLDF
  FWNETWYLKTIDICHDHFGWYLGWGDCVWLPYLYTLQGLYLVYHPVQLSTPNALGVLLLGLVGYYIFRMT
  NHQKDLFRRTDGHCLIWGKKPKAIECSYTSADGLKHRSKLLVSGFWGVARHFNYTGDLMGSLAYCLACGG
  GHLLPYFYIIYMTILLTHRCLRDEHRCANKYGRDWERYVAAVPYRLLPGIF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022389
• ORF Size: 1413 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_022389.1, NM_022389.2, NP_071784.1
• RefSeq Size: 2737
• RefSeq ORF: 1416
• Locus ID: 64191
• MW: 54.2 kDa
• Gene Summary: catalyzes the reduction of 7-dehydrocholesterol and involved in cholesterol biosynthesis [RGD, Feb 2006]